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Fig. 1. Structures of Pro 3 -TL andPro 3 -dLeu 9 -TL.Table 2. Antimicrobial activities of Temporin L analogues (MIC mM)ConclusionThis preliminarily study has highlighted that the amino acids Lys7, Gly10, and Ile12 couldbe important for antimicrobial and hemolytic activity. In fact, the peptides TL-D1, TL-D6,and TL-D7 showed to have a reduced biological activity in respect to the native peptide.Further biological studies are under investigation to evaluate the ability of these peptides toinhibit the growth of further Gram-positive, Gram-negative and yeast species and toevaluate their hemolytic activity.AcknowledgmentsThe LC-MS and NMR spectral data were provided by Centro di Ricerca Interdipartimentale di AnalisiStrumentale, Università degli Studi di Napoli “Federico II”. The assistance of the staff is gratefullyappreciated.References1 Shai, Y. Biopolymers 66, 236-248 (2002).2 Mangoni, M.L., Rinaldi, A.C., Di Giulio, A., Mignogna, G., Bozzi, A., Barra, D., Simmaco, M. Eur.J. Biochem. 267, 1447-1454 (2000).3 Carotenuto, A., Malfi, S., Saviello, M.R., Campiglia, P., Gomez-Monterrey, M.I., et.al. J. Med.Chem. 51, 2354-2362 (2008).4 Saviello, M.R., Malfi, S., Campiglia, P., Cavalli, A., Grieco, P., Novellino, E., Carotenuto, A.Biochemistry 49, 1477-1485 (2010).361