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Proceedings of the 31 st European Peptide SymposiumMichal Lebl, Morten Meldal, Knud J. Jensen, Thomas Hoeg-Jensen (Editors)European Peptide Society, 2010A Regulatory Role for the Muramyl Peptide (GMDP) in aMurine Model of Allergic AsthmaSvetlana V. Guryanova 1 , Marina A. Shevchenko 1 , Ivan G. Kozlov 2 ,and Tatyana M. Andronova 11 Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, 117997,Russian Federation; 2 Russian State Medical University, Moscow, 117997,Russian FederationIntroductionN-acetylglucosaminyl-N-acetylmuramyl dipeptide (GMDP) (Figure 1) – fragment ofbacterial cell wall peptidoglycan is known to possess immunomodulatory activity againstviral and bacterial infection [1,2].Positive effect of based on GMDP pharmaceutics Licopid application was shown atthe treatment of children with bronchial asthma and atopic dermatitis [3]. Previously wereported that in vitro studies on periphery blood mononuclear cells of allergic bronchialasthma patients demonstrated the ability of GMDP to shift Th1/Th2 balance towards Th1and IFN- production [4].In the present study we evaluated the ability of GMDP to modulate allergic airwayinflammation both on the stage of sensitization and during ongoing airway inflammation.These results suggest that antiasthmatic activity of GMDP in OVA-induced lunginflammation may occur in part via downregulation of IgE production and eosinophilairway infiltration.Results and DiscussionThe standard well-characterized OVA-induced mouse model of asthma [5] (OVA/OVA)was utilized to assess the immunomodulatoryeffect of GMDP. Mice wereimmunized with GMDP in the stage ofsensitization (GMDP/OVA/OVA) – twodays before each intraperitoneal (i.p.)OVA/Alum injection or during effectorstage of the airway inflammation - threeconsequent i.p. injections between thesecond and the last OVA challenge(OVA/OVA/GMDP).Twofold decrease of serum total IgEFig. 1. N-acetylglucosaminyl-N-acetylmuramyldipeptide (GMDP).and broncho-alveolar lavage fluid (BALF)IgA was detected in GMDP/OVA/OVAmice compared to the OVA/OVA group(Figure 2B). At the same time such route ofOD4502.52.01.51.00.5* †††**†††IgG1IgG2aBOD4501.51.00.5††******††††IgEIgA0.0GMDP/OVA/OVAOVA/OVA/GMDPOVA/OVAGMDPNM0.0GMDP/OVA/OVAOVA/OVA/GMDPOVA/OVAFig. 2. Immunoglobulin production. For IgG detection sera were diluted 1:1000, for IgE1:10. IgA was detected in BALF (1:1). *P < 0.05, **P < 0.01, ***P < 0.005 vs.OVA/OVA; † P < 0.005 vs NM.GMDPNM568