User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent

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Regulatory and Safety InformationWARNING! Potential System Damage: Do not disconnect an electrical assembly whilepower is applied to the system. Once the power is turned off, wait approximately 30seconds before disconnecting an assembly.WARNING! Potential System Damage: Do not expose the system to excessive voltagefluctuations or power surges. The system contains a number of sensitive electroniccomponents that may be damaged.Qualified PersonnelAfter installing the system, the Field Service Employee (FSE) uses the Customer FamiliarizationChecklist to familiarize the customer on system operation, cleaning, and basic maintenance.Only trained personnel shall install, operate, and maintain the equipment. Equipment serviceshall only be conducted by FSEs or AB SCIEX authorized dealers. Contact AB SCIEX TechnicalSupport for more information or visit www.absciex.com.Equipment Use and ModificationUse the system indoors in a laboratory that complies with the environmental conditionsrecommended in the Site Planning Guide. If the system is used in an environment or in a mannernot prescribed by AB SCIEX, the protection provided by the equipment can be impaired.Unauthorized modification or operation of the system may cause personal injury and equipmentdamage.Caution: Potential System Damage: Contact the manufacturer prior to making changesor modifications to the system. Changes or modifications to this unit not expresslyapproved by the manufacturer could void the instrument warranty and render the systeminoperable.Contact an AB SCIEX representative for more information on servicing the instrument.Regulatory ComplianceThis system complies with the standards and regulations listed in this section. Applicable labelshave been affixed to the system. For more information, refer to the Declaration of Conformanceincluded with the system.CanadaEurope• Safety—CAN/CSA C22.2 No. 61010-1• Electromagnetic Compatibility—ICES-001• Safety—EN 61010-1• Electromagnetic Compatibility—EN 55011 Class A and EN 61326-1• WEEE—2002/96/EECekspert nanoLC 400 SystemsOperator Guide12 of 174 D5033460 C
Regulatory and Safety InformationUSA• Safety—UL 61010-1• Electromagnetic Compatibility—FCC PG 15 Class AInternational• Safety—IEC 61010-1• Electromagnetic Compatibility—CISPR 11 Class A, IEC 61326-1Symbols and LabelsTable 1-2Symbols and LabelsExternal LabelsDefinitionCaution: Risk of needle-stick puncture.Take care that air circulation is not impeded.WARNING: Possible eye injury due to hazardous opticalradiation.Do not look directly into the cabinet lamp.Do not dispose of equipment as unsorted municipal waste(WEEE).Direct CurrentAVV-AAmperes (current)Volts (voltage)Volts - Amperes (power)Operator Guideekspert nanoLC 400 SystemsD5033460 C 13 of 174
Page 1 and 2: ekspert nanoLC 400 SystemsOperator
Page 3: Revision LogRevision Reason for Cha
Page 6 and 7: ContentsCreate the LC Method—Grad
Page 8 and 9: ContentsThe Elution Method . . . .
Page 10 and 11: Regulatory and Safety InformationSa
Page 14 and 15: Regulatory and Safety InformationSy
Page 16 and 17: IntroductionAccessory OptionsDepend
Page 18 and 19: System OverviewFigure 3-2Back Panel
Page 20 and 21: System Overview2. Click Devices > A
Page 22 and 23: System OverviewCaution: Potential S
Page 24 and 25: System Overviewand forced out of th
Page 26 and 27: System Overviewb. Place a new label
Page 28 and 29: System Overview7. Specify an approp
Page 30 and 31: System OverviewThe column begins to
Page 32 and 33: System OverviewFigure 3-13 Hardware
Page 34 and 35: System OverviewFigure 3-15 Appearan
Page 36 and 37: System OverviewThe sample loop is i
Page 38 and 39: System OverviewTable 3-4FlowrateDif
Page 40 and 41: System OverviewCreate the Autosampl
Page 42 and 43: System OverviewNote: Refer to Creat
Page 44 and 45: System OverviewFigure 3-24 LC Metho
Page 46 and 47: System OverviewIf necessary, click
Page 54 and 55: System Overview8. In the next line
Page 56 and 57: System OverviewStop a Run in the Ru
Page 58 and 59: System OverviewView the Data FilesP
Page 60 and 61: Moving the SystemCommunication with
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Moving the Systemekspert nanoLC 400
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System MaintenanceDispose of System
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System MaintenanceFlush the Systemb
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System MaintenanceRequired Material
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System Maintenance• Low-flow chan
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System MaintenanceReplace the Syrin
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System Maintenanceb. Apply tension
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System MaintenanceReplace the Sampl
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System MaintenanceReplace the Punct
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System MaintenanceReplace the Rotor
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System Maintenanceekspert nanoLC 40
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Best Practices and Troubleshooting
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingT
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System Specificationsekspert nanoLC
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System Specificationsekspert nanoLC
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Theory of OperationHow the MFC Plus
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External Interface ConnectionsPin A
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External Interface ConnectionsPin A
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External Interface ConnectionsA/D C
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Standard Plumbing DiagramsFigure D-
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Standard Plumbing DiagramsFigure D-
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Autosampler Method Editor4. Click O
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Autosampler Method EditorCreate an
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Autosampler Method Editor• Click
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Autosampler Method EditorTable E-2D
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Autosampler Method EditorNeedle Was
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Basic MethodsFigure F-2Advanced Met
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Basic MethodsFigure F-4LC Method Se
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Basic MethodsTrap-and-elute MethodF
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Basic MethodsFigure F-8LC Method Se
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Basic MethodsFigure F-10 LC Method
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Basic MethodsFigure F-12 LC Method
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Basic MethodsFigure F-14 Run Manage
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Basic Methodsekspert nanoLC 400 Sys
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Working with Analyst® SoftwareVeri
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Working with Analyst® SoftwareFigu
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Working with Analyst® SoftwareFigu
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Working with Analyst® Softwareeksp
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2-D Reverse-phase/Reverse-phase Liq
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IndexFflow calibration assembly 36f
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Indextimer 36torque wrench 28traces
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User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent