User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewFigure 3-3Acquisition WindowChannel Assignments in the Eksigent Control SoftwareThe ekspert nanoLC 415 and 425 systems have two or three pump channels, respectively. Thechannels are identified in this guide and in the software as Gradient 1, Gradient 2, and Loadingpump. Refer to Table 3-1 for channel assignments.In the software, select the channel in an active dialog or window by clicking the arrow beside thechannel. The channel typically shows in the top, right corner of a dialog or window.Table 3-1Channel Assignments with Nanoflow Modules in the Gradient ChannelsSystem Gradient 1 Gradient 2 Loading Pump415 Nanoflow (100 nL/min to1000 nL/min)425 Nanoflow (100 nL/min to1000 nL/min)N/ANanoflow (100 nL/min to1000 nL/min)Microflow (1 µL/min to50 µL/min)Microflow (1 µL/min to50 µL/min)Note: The flow rates in Table 3-1 are for the default flow modules. If different flowmodules are installed on the system, the flow rates will differ.Autosampler ConfigurationThe AB SCIEX FSE configures the autosampler during installation.Verify the Configuration Settings1. Open the Run Manager.Operator Guideekspert nanoLC 400 SystemsD5033460 C 19 of 174