User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewEquilibrate the SystemPrerequisite Procedure• Replace the Mobile PhasesUse the Direct Control dialog to equilibrate the system following system power-up, a change ofsolvent, or a change of column. Open the Autosampler Configuration window to toggle theinjection valve between load and inject positions. Toggling flushes the sample loop andinterconnecting ports.1. In the Acquisition window, select the appropriate channel in the top right corner ofthe window.2. Click System > Direct Control.Figure 3-16 Direct Control Dialog3. Make sure that Conserved Flow is selected and set A (%) and B (%) to 90 and 10,respectively.This is the mobile phase composition used for equilibration.Use Conserved Flow to choose a total flowrate and percent composition. UseIndependent Flow to select independent flowrates for A and B. Conserved Flow ismost commonly used with the ekspert nanoLC 400 systems.4. Set the Total flowrate to 400 (nL/min) or other appropriate flowrate.5. Click Start to start the pump flowing and begin equilibration.6. In the Acquisition window, click Devices > Autosampler Device Settings.7. Flush the autosampler switching valve:a. Click the Direct Control tab.b. In the Injection Valve group, flush the switching valve by alternately clickingLoad Position and Inject Position to release trapped air.Operator Guideekspert nanoLC 400 SystemsD5033460 C 35 of 174