User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System Overview5. Pull down the slide on the flow module cartridge to protect the fluidic connectorsduring storage.6. Push up the slide on the new flow module cartridge.7. Insert the new flow module cartridge in the system. It can only go in one way.8. Using the calibrated torque wrench, secure the cartridge.Hold the wrench only by the break-over handle to obtain the calibrated results.9. Flush the module with a minimum of 50 µL of the current solvent.When you change the modules, the pump may seem to have zero pressure on Pc.Because Pc is used to calculate flow, the channel may initially not seem to pump. Itis actually pumping out the air introduced during the module change. Once the air isgone, Pc will come up, the liquid begins flowing through the channel, and thesoftware shows normal pressures.Set the Column Oven TemperatureThe column oven is an optional component. It is associated with a single Channel. On the backpanel, the column oven connects to Channel 1.1. Click System > Direct Control.2. In the Direct Control dialog, select Gradient 1.3. Type the column Setpoint temperature (for example, 40°C).Figure 3-10 Direct Control Dialog—Setpoint Temperature4. In the Column Oven/Heater group, click Start.Operator Guideekspert nanoLC 400 SystemsD5033460 C 29 of 174