User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System Overview5. On the Run Conditions tab, in the Pre-Run group, select the Flush column checkbox and specify a time appropriate to equilibrate the pump, connected tubing, andcolumn, using 100% initial flowrate conditions.Figure 3-31 LC Method Settings Dialog—Run Conditions Tab6. In the Sample Injection group, click Standard. Refer to Standard and MeteredInjection Parameters on page 44.7. Make sure that the check box in Post-Run group is cleared.8. On the Gradient Table tab, specify the gradient parameters as shown in Figure 3-32.• Add new steps by clicking the >> to the left of the table.• Delete steps by clicking the X.• Set the Total flowrate on the right side of the dialog.Operator Guideekspert nanoLC 400 SystemsD5033460 C 51 of 174