User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System Overview7. Save the method.8. Click OK to close the Method Editor dialog.Note: Refer to Create an Advanced Method on page 122 for a detaileddescription of how to create an advanced method.Create the LC Method—Loading Pump1. In the LC Channel column, select Loading pump.2. In the Run Manager window, click LC Methods.Figure 3-27 LC Method Settings Dialog—Summary Tab3. In the Name field, type a method name and click Save.4. (Optional) Specify column information appropriate to the experiment.This information is stored with the LC method file.5. On the Run Conditions tab, in the Pre-Run group, select the Flush column checkbox and specify a time of 0.1 minutes using 100% initial flowrate conditions.Operator Guideekspert nanoLC 400 SystemsD5033460 C 47 of 174