User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent

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Regulatory and Safety InformationSystem Disposal (Waste Electrical and ElectronicEquipment)Do not dispose of system components or subassemblies, including computer parts, as unsortedmunicipal waste. Follow local municipal waste ordinances for proper disposal provisions toreduce the environmental impact of WEEE (waste, electrical, and electronic equipment). Tosafely dispose of this equipment, contact an FSE for instructions.European Union customers: Contact a local AB SCIEX Customer Service office forcomplimentary equipment pick-up and recycling.Related Documentation• Site Planning Guide—available from www.eksigent.com• Eksigent Control Software User Guide—installed with the Eksigent control software• Analyst ® Software Getting Started Guide or System User Guide—installed with theAnalyst ® softwareTechnical SupportAB SCIEX and its representatives maintain a staff of fully-trained service and technicalspecialists located throughout the world. They can answer questions about the instrument or anytechnical issues that may arise. For more information, visit the web site at www.absciex.com.ekspert nanoLC 400 SystemsOperator Guide14 of 174 D5033460 C
Introduction2The Eksigent ekspert nanoLC 400 systems are designed for HPLC applications that employdirect pumping at flowrates of several hundred nL/min up to µL/min.The ekspert nanoLC 415 and 425 systems include:• Binary gradient pumping system with loading pump. The flowrates depend on theinstalled flow modules.• Eksigent control software• ekspert nanoLC 400 autosampler• Autosampler solvent trayFigure 2-1ekspert nanoLC 425 system123Item Description1 Solvent tray2 Autosampler3 PumpOperator Guideekspert nanoLC 400 SystemsD5033460 C 15 of 174
Page 1 and 2: ekspert nanoLC 400 SystemsOperator
Page 3: Revision LogRevision Reason for Cha
Page 6 and 7: ContentsCreate the LC Method—Grad
Page 8 and 9: ContentsThe Elution Method . . . .
Page 10 and 11: Regulatory and Safety InformationSa
Page 12 and 13: Regulatory and Safety InformationWA
Page 16 and 17: IntroductionAccessory OptionsDepend
Page 18 and 19: System OverviewFigure 3-2Back Panel
Page 20 and 21: System Overview2. Click Devices > A
Page 22 and 23: System OverviewCaution: Potential S
Page 24 and 25: System Overviewand forced out of th
Page 26 and 27: System Overviewb. Place a new label
Page 28 and 29: System Overview7. Specify an approp
Page 30 and 31: System OverviewThe column begins to
Page 32 and 33: System OverviewFigure 3-13 Hardware
Page 34 and 35: System OverviewFigure 3-15 Appearan
Page 36 and 37: System OverviewThe sample loop is i
Page 38 and 39: System OverviewTable 3-4FlowrateDif
Page 40 and 41: System OverviewCreate the Autosampl
Page 42 and 43: System OverviewNote: Refer to Creat
Page 44 and 45: System OverviewFigure 3-24 LC Metho
Page 46 and 47: System OverviewIf necessary, click
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Page 56 and 57: System OverviewStop a Run in the Ru
Page 58 and 59: System OverviewView the Data FilesP
Page 60 and 61: Moving the SystemCommunication with
Page 62 and 63: Moving the Systemekspert nanoLC 400
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System MaintenanceDispose of System
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System MaintenanceFlush the Systemb
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System MaintenanceRequired Material
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System Maintenance• Low-flow chan
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System MaintenanceReplace the Syrin
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System Maintenanceb. Apply tension
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System MaintenanceReplace the Sampl
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System MaintenanceReplace the Punct
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System MaintenanceReplace the Rotor
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System Maintenanceekspert nanoLC 40
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Best Practices and Troubleshooting
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingT
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System Specificationsekspert nanoLC
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System Specificationsekspert nanoLC
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Theory of OperationHow the MFC Plus
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External Interface ConnectionsPin A
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External Interface ConnectionsPin A
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External Interface ConnectionsA/D C
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Standard Plumbing DiagramsFigure D-
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Standard Plumbing DiagramsFigure D-
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Autosampler Method Editor4. Click O
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Autosampler Method EditorCreate an
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Autosampler Method Editor• Click
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Autosampler Method EditorTable E-2D
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Autosampler Method EditorNeedle Was
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Basic MethodsFigure F-2Advanced Met
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Basic MethodsFigure F-4LC Method Se
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Basic MethodsTrap-and-elute MethodF
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Basic MethodsFigure F-8LC Method Se
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Basic MethodsFigure F-10 LC Method
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Basic MethodsFigure F-12 LC Method
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Basic MethodsFigure F-14 Run Manage
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Basic Methodsekspert nanoLC 400 Sys
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Working with Analyst® SoftwareVeri
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Working with Analyst® SoftwareFigu
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Working with Analyst® SoftwareFigu
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Working with Analyst® Softwareeksp
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2-D Reverse-phase/Reverse-phase Liq
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IndexFflow calibration assembly 36f
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Indextimer 36torque wrench 28traces
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User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent