User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System Overview7. In the Sample Injection group, click Metered.This option signals the injection valve to be placed in the inject position for theduration of the Metered Injection (that is, 10000 nL). Refer to Standard and MeteredInjection Parameters on page 44.8. Make sure that the check box in the Post-Run group is cleared.9. On the Gradient Table tab, specify the gradient parameters as shown in Figure 3-23.• Add new steps to the table by clicking the arrows (>>) to the left of the table.• Delete steps by clicking the X.• Set the Total flowrate on the right side of the dialog.• If events such as Lamp on/off, Valve state toggles, or Output switching arerequired, specify them in the Events column.Figure 3-23 LC Method Settings Dialog—Gradient Table Tab10. On the Gradient Profile tab, review the gradient to make sure it is correct.11. When the method is complete, click Save.12. Click OK to close the editor.Operator Guideekspert nanoLC 400 SystemsD5033460 C 43 of 174