User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent

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System OverviewFigure 3-28 LC Method Settings Dialog—Run Conditions Tab6. In the Sample Injection group, click None.Refer to Create the LC Method—Gradient on page 50.7. In the Post-Run group, make sure that the check box is cleared.8. On the Gradient Table tab, specify the loading pump parameters.• Type the appropriate Qa (flowrate) and Time.In this method, Time is the amount of time that the Loading pump is flowingthrough the sample loop, pushing liquid over to the trap column. Make surethat this time is long enough to properly load the sample onto the trap andwash away any salts or detergents.ekspert nanoLC 400 SystemsOperator Guide48 of 174 D5033460 C
System OverviewFigure 3-29 LC Method Settings Dialog—Gradient Table Tab9. On the Gradient Profile tab, review the gradient to make sure it is correct.Operator Guideekspert nanoLC 400 SystemsD5033460 C 49 of 174
Page 1 and 2: ekspert nanoLC 400 SystemsOperator
Page 3: Revision LogRevision Reason for Cha
Page 6 and 7: ContentsCreate the LC Method—Grad
Page 8 and 9: ContentsThe Elution Method . . . .
Page 10 and 11: Regulatory and Safety InformationSa
Page 12 and 13: Regulatory and Safety InformationWA
Page 14 and 15: Regulatory and Safety InformationSy
Page 16 and 17: IntroductionAccessory OptionsDepend
Page 18 and 19: System OverviewFigure 3-2Back Panel
Page 20 and 21: System Overview2. Click Devices > A
Page 22 and 23: System OverviewCaution: Potential S
Page 24 and 25: System Overviewand forced out of th
Page 26 and 27: System Overviewb. Place a new label
Page 28 and 29: System Overview7. Specify an approp
Page 30 and 31: System OverviewThe column begins to
Page 32 and 33: System OverviewFigure 3-13 Hardware
Page 34 and 35: System OverviewFigure 3-15 Appearan
Page 36 and 37: System OverviewThe sample loop is i
Page 38 and 39: System OverviewTable 3-4FlowrateDif
Page 40 and 41: System OverviewCreate the Autosampl
Page 42 and 43: System OverviewNote: Refer to Creat
Page 44 and 45: System OverviewFigure 3-24 LC Metho
Page 46 and 47: System OverviewIf necessary, click
Page 54 and 55: System Overview8. In the next line
Page 56 and 57: System OverviewStop a Run in the Ru
Page 58 and 59: System OverviewView the Data FilesP
Page 60 and 61: Moving the SystemCommunication with
Page 62 and 63: Moving the Systemekspert nanoLC 400
Page 64 and 65: System MaintenanceDispose of System
Page 66 and 67: System MaintenanceFlush the Systemb
Page 68 and 69: System MaintenanceRequired Material
Page 70 and 71: System Maintenance• Low-flow chan
Page 72 and 73: System MaintenanceReplace the Syrin
Page 74 and 75: System Maintenanceb. Apply tension
Page 76 and 77: System MaintenanceReplace the Sampl
Page 78 and 79: System MaintenanceReplace the Punct
Page 80 and 81: System MaintenanceReplace the Rotor
Page 82 and 83: System Maintenanceekspert nanoLC 40
Page 84 and 85: Best Practices and Troubleshooting
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Page 88 and 89: Best Practices and TroubleshootingF
Page 90 and 91: Best Practices and Troubleshooting
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Best Practices and TroubleshootingT
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System Specificationsekspert nanoLC
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System Specificationsekspert nanoLC
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Theory of OperationHow the MFC Plus
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External Interface ConnectionsPin A
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External Interface ConnectionsPin A
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External Interface ConnectionsA/D C
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Standard Plumbing DiagramsFigure D-
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Standard Plumbing DiagramsFigure D-
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Autosampler Method Editor4. Click O
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Autosampler Method EditorCreate an
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Autosampler Method Editor• Click
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Autosampler Method EditorTable E-2D
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Autosampler Method EditorNeedle Was
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Basic MethodsFigure F-2Advanced Met
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Basic MethodsFigure F-4LC Method Se
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Basic MethodsTrap-and-elute MethodF
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Basic MethodsFigure F-8LC Method Se
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Basic MethodsFigure F-10 LC Method
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Basic MethodsFigure F-12 LC Method
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Basic MethodsFigure F-14 Run Manage
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Basic Methodsekspert nanoLC 400 Sys
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Working with Analyst® SoftwareVeri
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Working with Analyst® SoftwareFigu
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Working with Analyst® SoftwareFigu
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Working with Analyst® Softwareeksp
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2-D Reverse-phase/Reverse-phase Liq
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IndexFflow calibration assembly 36f
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Indextimer 36torque wrench 28traces
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User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent