User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent

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System OverviewThe column begins to heat. The current temperature is visible in the upper, rightcorner of the Acquisition window for Gradient 1 when the oven is installed and runon.Figure 3-11 Acquisition Window—Column Oven TemperatureRefer to the Eksigent Control Software User Guide for information on using thecolumn oven to explore the impact of varying column temperature in the RunManager window. The Run Manager window allows a queue to be set up anddifferent column temperatures to be entered for each run.Caution: Potential System Damage: Do not leave the column oven coveropen for long periods of time while the column oven is operating. The heaterunit is designed to operate as a closed system. Leaving the cover open withthe heater on at high temperatures may damage the heater system.Configure the A/D ConverterAn optional A/D converter is required for connection to external detectors such as UV orfluorescence detectors. The FSE installs the optional A/D converter during installation of theekspert nanoLC 400 system.The section assumes the ekspert nanoLC 400 system is connected to a computer and that theEksigent control software has been installed. By default, all the software is installed for the A/Ddevice when the Eksigent software package is installed on the control computer.ekspert nanoLC 400 SystemsOperator Guide30 of 174 D5033460 C
System OverviewFigure 3-12 A/D ConverterItem Description1 Screw terminal pins (21-40)2 LED*3 Screw terminal pins (1-20)4 USB connector* If the LED is on with a steady green, then the device is connected to a computer or externalUSB hub. If the LED blinks once, then a USB command has been received by the device.Continuously blinking indicates an analog input scan is taking place.Set the Scale and A/D Input Voltage RangeSelection1. Open the Eksigent control software.2. Select System > Hardware Diagnostics.3. On the External A/D tab, select the A/D Input Voltage Range Selection from thelist.Operator Guideekspert nanoLC 400 SystemsD5033460 C 31 of 174
Page 1 and 2: ekspert nanoLC 400 SystemsOperator
Page 3: Revision LogRevision Reason for Cha
Page 6 and 7: ContentsCreate the LC Method—Grad
Page 8 and 9: ContentsThe Elution Method . . . .
Page 10 and 11: Regulatory and Safety InformationSa
Page 12 and 13: Regulatory and Safety InformationWA
Page 14 and 15: Regulatory and Safety InformationSy
Page 16 and 17: IntroductionAccessory OptionsDepend
Page 18 and 19: System OverviewFigure 3-2Back Panel
Page 20 and 21: System Overview2. Click Devices > A
Page 22 and 23: System OverviewCaution: Potential S
Page 24 and 25: System Overviewand forced out of th
Page 26 and 27: System Overviewb. Place a new label
Page 28 and 29: System Overview7. Specify an approp
Page 32 and 33: System OverviewFigure 3-13 Hardware
Page 34 and 35: System OverviewFigure 3-15 Appearan
Page 36 and 37: System OverviewThe sample loop is i
Page 38 and 39: System OverviewTable 3-4FlowrateDif
Page 40 and 41: System OverviewCreate the Autosampl
Page 42 and 43: System OverviewNote: Refer to Creat
Page 44 and 45: System OverviewFigure 3-24 LC Metho
Page 46 and 47: System OverviewIf necessary, click
Page 54 and 55: System Overview8. In the next line
Page 56 and 57: System OverviewStop a Run in the Ru
Page 58 and 59: System OverviewView the Data FilesP
Page 60 and 61: Moving the SystemCommunication with
Page 62 and 63: Moving the Systemekspert nanoLC 400
Page 64 and 65: System MaintenanceDispose of System
Page 66 and 67: System MaintenanceFlush the Systemb
Page 68 and 69: System MaintenanceRequired Material
Page 70 and 71: System Maintenance• Low-flow chan
Page 72 and 73: System MaintenanceReplace the Syrin
Page 74 and 75: System Maintenanceb. Apply tension
Page 76 and 77: System MaintenanceReplace the Sampl
Page 78 and 79: System MaintenanceReplace the Punct
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System MaintenanceReplace the Rotor
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System Maintenanceekspert nanoLC 40
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Best Practices and Troubleshooting
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingT
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System Specificationsekspert nanoLC
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System Specificationsekspert nanoLC
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Theory of OperationHow the MFC Plus
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External Interface ConnectionsPin A
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External Interface ConnectionsPin A
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External Interface ConnectionsA/D C
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Standard Plumbing DiagramsFigure D-
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Standard Plumbing DiagramsFigure D-
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Autosampler Method Editor4. Click O
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Autosampler Method EditorCreate an
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Autosampler Method Editor• Click
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Autosampler Method EditorTable E-2D
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Autosampler Method EditorNeedle Was
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Basic MethodsFigure F-2Advanced Met
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Basic MethodsFigure F-4LC Method Se
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Basic MethodsTrap-and-elute MethodF
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Basic MethodsFigure F-8LC Method Se
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Basic MethodsFigure F-10 LC Method
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Basic MethodsFigure F-12 LC Method
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Basic MethodsFigure F-14 Run Manage
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Basic Methodsekspert nanoLC 400 Sys
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Working with Analyst® SoftwareVeri
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Working with Analyst® SoftwareFigu
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Working with Analyst® SoftwareFigu
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Working with Analyst® Softwareeksp
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2-D Reverse-phase/Reverse-phase Liq
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IndexFflow calibration assembly 36f
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Indextimer 36torque wrench 28traces
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User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent