User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewCreate Autosampler and LC Methods—Trap-and-EluteComplete the following procedures:1. Create the Autosampler Method.2. Create the LC Method—Loading Pump.3. Create the LC Method—Gradient.Create the Autosampler MethodThe parameters used for loading the sample into the injection valve and for rinsing theautosampler syringe and sample needle are stored in the autosampler method. This sectioncreates an autosampler method appropriate for loading a trap column using the loading pump,and running a gradient with Gradient 1.1. Place the sample vial containing the standard test mixture in vial position C01 ofautosampler 108-vial tray.2. In the Acquisition window, click Run Manager.Figure 3-25 Run Manager WindowTip! If the current tray does not display a picture of the 108-vial tray in theRun Manager window, click Devices > Autosampler Device Settings andselect the tray type. Open the Run Manager window after changing the traytype.3. Make sure the columns in Figure 3-25 appear in the Run Manager window.Operator Guideekspert nanoLC 400 SystemsD5033460 C 45 of 174