User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewFigure 3-19 Autosampler Configuration Dialog—Configuration TabParameterTypical ValueLoop Volume10 µL (dependent on the installed hardware)Needle and Tubing Volume 3.6 µLSyringe Volume50 µL (standard installed syringe volume)Wash Speed2 µL/minNote: With the exception of Wash Speed, the values in Figure 3-19 are dependent onthe hardware installed.Create Autosampler and LC Methods—DirectInjectionComplete the following procedures:1. Create the Autosampler Method.2. Create the LC Method—Gradient.Operator Guideekspert nanoLC 400 SystemsD5033460 C 39 of 174