User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewCaution: Potential System Damage: If the pump is dry, manually prime thepump. Refer to Prime the Pump.6. For a wet pump, proceed to purge and flush the system. Refer to Purge the Pumpand Flush the Pump.Specify the Maximum FlowrateThe Maximum Flowrate is automatically set when a flow module is inserted. For most nanoflowapplications, 1000 nL/min is appropriate, as shown in Figure 3-7.Figure 3-7Hardware Options Tab—Maximum Flowrate1. Click System > Instrument Configuration.2. On the Hardware Options tab, click the correct channel.3. Select the Allow Customization check box.4. Type a Maximum Flowrate in the field.5. Click OK.Prime the PumpPriming is required for dry pumps. It should not be required under normal operating conditions.This priming procedure may also be used to rapidly exchange solvents.Priming the pump requires manual intervention. This procedure involves manually drawingsolvent through the fluidic path with a 5 mL syringe. While the priming syringe minimizesleachables and extractables with commonly used HPLC solvent systems, the solvent drawn intoOperator Guideekspert nanoLC 400 SystemsD5033460 C 23 of 174