User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent

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System Overviewb. Place a new labelled bottle filled with solvent near the solvent bottle to bechanged out.c. In the Eksigent control software, click System > Mobile Phases > MORE andspecify the channel (that is, Gradient 1, Gradient 2, or Loading pump).d. In the Purge Settings group, select the solvent (A or B) to be changed.e. In the mobile phase bottle, pull the inlet frit out of the liquid so that the filter canbe cleared of old solvent.f. Specify 5 purge cycles, and click Purge Now.g. As the pump draws liquid up the line, an air gap forming in the line at one endof the reservoir.h. Remove the old bottle when the end of the line is clear of solvent.i. Replace the old bottle with a new bottle and place the mobile phase filters intothe new liquid.3. Proceed to step 2 in the section, Prime a Dry Pump.Purge the PumpPurging rapidly replaces the solvent in the pump. Repeat this procedure for all channels, ifnecessary.Prerequisite Procedure• Replace the Mobile Phases1. In the Acquisition window, click System > Mobile Phases.2. Verify that the software settings for solvent composition match the actual solvents inthe bottles.• Binary mixture A sets the composition for Solvent A (mobile phase).• Binary mixture B sets the composition for Solvent B (mobile phase).3. If necessary, repeat step 2 for Gradient 2 and Loading pump.4. Click More to open the Purge Settings and Flush Settings.ekspert nanoLC 400 SystemsOperator Guide26 of 174 D5033460 C
System OverviewFigure 3-9Mobile Phases Dialog—Purge and Flush Settingsa. In the Purge Settings group, select the Side A or Side B check box for thepump to purge.b. Set the number of purge cycles to 20.c. Click Purge Now.The pump begins to execute purge cycles. While the pump is purging, verifythat the mobile phases are pulled through the mobile phase tubing to thepump.d. Locate the waste tubing of the pump being purged. After about 8 purges, themobile phase should be purged through the waste tubing.Flush the PumpFlushing replaces the solvent in the capillaries connecting the pump to the pump outlet throughthe flow modules. Repeat this procedure for all channels, if necessary.Prerequisite Procedure• Purge the Pump1. Disconnect the tubing from the pump outlet.2. Connect a waste line to the pump outlet.3. In the Acquisition window, select the appropriate channel in the top right corner ofthe window.4. Click System > Mobile Phases.5. Click More.6. In the Flush Settings group, type 50 (µL) for the Total Volume.Operator Guideekspert nanoLC 400 SystemsD5033460 C 27 of 174
Page 1 and 2: ekspert nanoLC 400 SystemsOperator
Page 3: Revision LogRevision Reason for Cha
Page 6 and 7: ContentsCreate the LC Method—Grad
Page 8 and 9: ContentsThe Elution Method . . . .
Page 10 and 11: Regulatory and Safety InformationSa
Page 12 and 13: Regulatory and Safety InformationWA
Page 14 and 15: Regulatory and Safety InformationSy
Page 16 and 17: IntroductionAccessory OptionsDepend
Page 18 and 19: System OverviewFigure 3-2Back Panel
Page 20 and 21: System Overview2. Click Devices > A
Page 22 and 23: System OverviewCaution: Potential S
Page 24 and 25: System Overviewand forced out of th
Page 28 and 29: System Overview7. Specify an approp
Page 30 and 31: System OverviewThe column begins to
Page 32 and 33: System OverviewFigure 3-13 Hardware
Page 34 and 35: System OverviewFigure 3-15 Appearan
Page 36 and 37: System OverviewThe sample loop is i
Page 38 and 39: System OverviewTable 3-4FlowrateDif
Page 40 and 41: System OverviewCreate the Autosampl
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Page 44 and 45: System OverviewFigure 3-24 LC Metho
Page 46 and 47: System OverviewIf necessary, click
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Page 56 and 57: System OverviewStop a Run in the Ru
Page 58 and 59: System OverviewView the Data FilesP
Page 60 and 61: Moving the SystemCommunication with
Page 62 and 63: Moving the Systemekspert nanoLC 400
Page 64 and 65: System MaintenanceDispose of System
Page 66 and 67: System MaintenanceFlush the Systemb
Page 68 and 69: System MaintenanceRequired Material
Page 70 and 71: System Maintenance• Low-flow chan
Page 72 and 73: System MaintenanceReplace the Syrin
Page 74 and 75: System Maintenanceb. Apply tension
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System MaintenanceReplace the Sampl
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System MaintenanceReplace the Punct
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System MaintenanceReplace the Rotor
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System Maintenanceekspert nanoLC 40
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Best Practices and Troubleshooting
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting
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Best Practices and TroubleshootingF
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Best Practices and Troubleshooting5
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Best Practices and TroubleshootingT
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System Specificationsekspert nanoLC
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System Specificationsekspert nanoLC
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Theory of OperationHow the MFC Plus
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External Interface ConnectionsPin A
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External Interface ConnectionsPin A
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External Interface ConnectionsA/D C
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Standard Plumbing DiagramsFigure D-
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Standard Plumbing DiagramsFigure D-
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Autosampler Method Editor4. Click O
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Autosampler Method EditorCreate an
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Autosampler Method Editor• Click
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Autosampler Method EditorTable E-2D
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Autosampler Method EditorNeedle Was
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Basic MethodsFigure F-2Advanced Met
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Basic MethodsFigure F-4LC Method Se
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Basic MethodsTrap-and-elute MethodF
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Basic MethodsFigure F-8LC Method Se
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Basic MethodsFigure F-10 LC Method
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Basic MethodsFigure F-12 LC Method
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Basic MethodsFigure F-14 Run Manage
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Basic Methodsekspert nanoLC 400 Sys
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Working with Analyst® SoftwareVeri
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Working with Analyst® SoftwareFigu
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Working with Analyst® SoftwareFigu
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Working with Analyst® Softwareeksp
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2-D Reverse-phase/Reverse-phase Liq
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IndexFflow calibration assembly 36f
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Indextimer 36torque wrench 28traces
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User Guide: ekspertâ¢ nanoLC 400 Systems Operator ... - Eksigent