User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System OverviewFigure 3-18 Direct Control Dialog—Flowrate Check4. In the top right corner of the dialog, select the appropriate channel.5. In the Direct Control dialog, for a Gradient pump, set channel A to 95% and anappropriate Total flowrate for that channel.• 5 µL/min for a high-flow channel• 500 nL/min for a low-flow channelFor the loading pump, set channel A to 100%.6. Click Start.7. When the liquid front reaches the black mark on the pipette, begin timing.• With the high flow calibration assembly, the time it takes for the liquidmeniscus to travel from the black stripe to the end of the capillary should be240 seconds.• With the low flow calibration assembly, the time it takes for the liquid meniscusto transit across two segments of the capillary (2 µL) should be 240 seconds.8. Click Stop in the Direct Control dialog when the liquid front reaches the end of thepipette or the appropriate black mark on the pipette.9. Inspect the flowrate to confirm that the system is working within an acceptablerange.Operator Guideekspert nanoLC 400 SystemsD5033460 C 37 of 174