User Guide: ekspert™ nanoLC 400 Systems Operator ... - Eksigent

System Overview4. Follow the instructions in the window to prime the pump.a. Stop all flow.b. On the front panel, turn the manual prime valve to Fill for the appropriatechannel.c. Fill the syringe with solvent by pulling the syringe until the syringe is full.This step pulls solvent from the bottle, through the line into the back of theinstrument, through the internal tubing and, finally, into the syringe body. Ittakes about 5 mL with a dry line for liquid to reach the syringe from the solventreservoir.d. Turn the manual prime valve to Prime.e. Slowly but firmly push the liquid into the pump.When approximately 2.5 mL of liquid has been pushed into the pump, liquidshould begin entering the waste container through the waste/purge externallines. This is an indication of proper priming.f. When liquid is entering the waste container, turn the manual prime valve toRun.g. The syringe may have some residual pressure in it. Unload or depressurizethe syringe by pulling back on the plunger. This prevents spilling or sprayingsolvent from the syringe body when disconnecting it from the system.h. Once the internal pressure of the syringe has been relieved, remove thesyringe from the pump by unscrewing the adapter tubing piece from thebulkhead on the front of the pump.5. Repeat these steps for each pump line to be primed.Exchange the SolventTip! Hold the syringe vertically with the plunger above the syringe tip tomake sure that any air that is in the syringe is not forced back into the pumpwhen the plunger is depressed in subsequent steps.If the pump is wet, miscible solvents may be changed. To introduce a solvent, identify andintroduce mutually miscible intermediate solvents prior to introducing the final solvents.Caution: Potential System Damage: Do not follow one solvent with an immisciblesolvent. Doing so may lead to a variety of undesirable results and unpredictable behaviorand potential instrument damage.If the pump is wet, introduce an air gap in the lines holding the existing liquid. This gap can stopthe new solvent from being contaminated with existing solvent in the line.1. In the Eksigent control software, stop all flows.Tip! Attaching the syringe at the beginning of this procedure helpsprevent leaks from the priming port when the prime valve is switched.2. Introduce an air gap.a. Identify the bottle and line to be changed.Operator Guideekspert nanoLC 400 SystemsD5033460 C 25 of 174