1 1 Symposium Chemosensory Receptors Satellite DEVELOPMENT ...

493 Poster Developmental, Neurogenesis, and ConsumerResearchOXIDATIVE STRESS-MEDIATED DEGENERATIVECHANGES IN THE AGING MURINE OLFACTORY BULBVaishnav R.A. 1 , Barnett K.R. 2 , Poon H.F. 3 , Hunter S.A. 1 , Hahn Y.K. 1 ,Getchell M.L. 2 , Butterfield D.A. 3 , Getchell T.V. 1 1 Physiology,University of Kentucky, Lexington, KY; 2 Anatomy and Neurobiology,University of Kentucky, Lexington, KY; 3 Chemistry, University ofKentucky, Lexington, KYOur recent proteomic analysis of the aging olfactory systemdemonstrated changes in several oxidative stress proteins. Here, wehave investigated oxidative stress markers in olfactory bulbs (OB) ofold (20 months) vs. young (1.5 months) mice. Total proteincarbonylation was significantly higher in OBs of old mice. 2D gelelectrophoresis and Western blotting detected over 300 oxidizedproteins. Specific carbonylation levels of 4 proteins identified by massspectrometry-based redox proteomics significantly differed in old vs.young mice, demonstrating the selectivity of age-associated changes inprotein oxidation. Of these, aldolase 1, localized in astroglia in thegranule cell layer, showed a 13-fold increase in carbonyl levels withaging. The oxidative stress management protein ferritin heavy chain 1was also carbonylated; it was up-regulated and localized in mitral/tuftedcells. Endothelial cells and microglia in the glomerular layer wereimmunoreactive for 3-nitrotyrosine, an oxidative protein modification,in OBs of old mice. We initiated GeneChip analyses to study globalchanges in the oxidative stress response. Genes regulated in the agingOB include key antioxidant proteins, chaperones and apoptosis pathwaymembers. Our results provide fundamental insight into the role ofoxidative stress in the aging OB. Support: AG-16824 (TVG); AG-05119 & AG-10836 (DAB).494 Poster Developmental, Neurogenesis, and ConsumerResearchODOR INCREASES INFANTS´ ATTENTION TO DYNAMICEMOTION DISPLAYHaviland-Jones J. 1 , Coffield C. 1 , Mayhew E. 1 , Walker-Andrews A. 21 Psychology, Rutgers, The State University of New Jersey, Piscataway,NJ; 2 Psychology, Montana State University, Missoula, MTInfants show specific preferences when it comes to looking atemotional expressions. Infants look longer at happy than sadexpressions (Walker-Andrews, 1977). Infants look longer to dynamicdisplays of emotion (Caron et al, 1988) and longer when displays havesound (Walker 1982). How might another sensory input (odor) affectinfants´ looking time? Thirty infants were shown both happy and saddynamic expressions (counterbalanced) in one of three conditions: noodor, pine or baby powder. Infants looked longer when they were in theodor conditions, regardless of the emotion displayed (p < 0.049). Thissuggests that the addition of the odor sensory channel increasesalertness and enables an infant to maintain visual attention, even tostimuli usually avoided (e.g., sad emotion expression).495 Poster Developmental, Neurogenesis, and ConsumerResearchEFFECTS OF AROMA ON AMATEUR TEN-PIN BOWLINGPERFORMANCEHirsch A.R. 1 , Ye Y. 2 , Lu Y. 3 , Choe M. 3 1 The Smell & Taste Treatmentand Research Foundation, Chicago, IL; 2 University of Illinois atChicago, Chicago, IL; 3 Illinois Mathematics and Science Academy,Aurora, ILIntroduction: While odor has been noted to have an impact in somesports, effects on bowling have never been addressed. Methods:Twenty subjects in a single-blind fashion bowled two frames. Oneframe was while wearing a blank surgical mask and the other was whilewearing a mask impregnated with the aroma of jasmine. These werepresented in a counterbalanced order. On an analog scale, subjects ratedthe hedonics of the jasmine aroma.Results: With the blank mask, theaverage score was 6.00, whereas while bowling with the jasmineimpregnated mask the average score was 8.35 (p = 0.0053). No effecton bowling score was seen in relation to absolute hedonics (p = 0.29),or relative hedonics—like/dislike (p = 0.64), of the jasminearoma.Conclusions: Ten-pin bowling in the presence of the odor ofjasmine improved scores. Possible mechanisms of action include moodregulation, enhanced alertness or concentration, anxiolysis, increasedself-confidence, and improved hand-eye coordination. A similar effectof jasmine may be observed in other sports requiring precise hand-eyecoordination or precision in execution.496 Poster Developmental, Neurogenesis, and ConsumerResearchFLORAL ODOR PROMPTS POSITIVE EMOTIONALSEARCHESWilson P. 1 , Coffield C. 2 , Haviland-Jones J. 2 1 Psychology, La SalleUniversity, Philadelphia, PA; 2 Psychology, Rutgers, The StateUniversity of New Jersey, Piscataway, NJFlowers preferred by humans increase Duchenne smiling and socialbehavior and decrease negative mood reports. Would a floral odor alsoaffect emotional behavior? 123 participants (66 female) rated their ownemotional state, rated the emotional response to short fear/anger videosand reported a recent memory in either a gardenia, peppermint or Etohcondition. There is no effect of odor on ratings of pleasantness orintensity (both odors are different from Etoh only).Condition did noteffect self-rating of mood. There is an effect of emotional response tothe fear/anger videos for positive emotion (p < 0.002) and negativeemotion (p < 0.02); both gardenia and peppermint prompt higherpositive emotion than Etoh but peppermint also prompts more negativeemotion than Etoh. There is an effect of odor on social reference wordsin the memory narrative (p < 0.02); gardenia prompts more than EtohThese results support the hypothesis that floral odor specializes insearches for positive emotion and social reference.124