RAINFOR GEM Intensive Plots Manual (pdf) - University of Oxford

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Two rhizotron transparencies following protocol #2 (example above from the SAFE plots, Malaysia; method C. Girardin). Roots aretraced onto a transparency held against the rhizotron screen (a different one each time). Different diameter classes are indicated bycolour (black-blue-red-green convention), but a new transparency is used each session and all roots are redrawn, which allows tickmarks and session numbers to be omitted (exclude roots/segments which branch after contacting the rhizotron screen). Note thetransparency ID top now includes the date (plot code/date of session/rhizotron no.). The right-hand transparency was drawn 2.5months after the left-hand one, showing that (unusually) many fine roots have died between these two time points.Both protocols described here work well in the field so it is up to you which you follow. The advantage ofprotocol #1 is that much less time is spent drawing roots each session. The advantage of protocol #2 is that (a)transparencies can be simply scanned and therefore the image analysis step should be much quicker e.g. usingWINRHIZO software http://www.regent.qc.ca/ 53 ) and (b) roots moving within the soil between sessions are nolonger a problem as they are when following protocol #1.53 See examples at http://www.plantstress.com/methods/Root%20Methods%20PORTAL/Root%20length%20method%20PSU.htm.Other alternatives are RootLM (http://digital.cs.usu.edu/~xqi/RootLM/) or ImageJ (http://rsb.info.nih.gov/ij/, see §4.3).42
Analysis: First, calculate the sum of expected elliptical cross-sections of observed roots that would cross therhizotron screen (XSr, mm 2 ) using the following equations (from Bernier & Robitaille 2004):∑2 23.1416 * rXSr =where r comes from:2Root diameter class3 mmAssumed mean root diameter (mm) 0.5 1.5 2.5 3.5so Mean root radius r (mm) 0.25 0.75 1.25 1.75Count the number of sections of root of each size class (e.g. 55 live root sections
Page 1: Measuring Tropical ForestCarbon All
Page 5 and 6: ContentsTHE RAINFOR-GEM INTENSIVE C
Page 7 and 8: The RAINFOR-GEM Intensive Carbon Mo
Page 9 and 10: What are the RAINFOR-GEM protocols?
Page 11 and 12: Where can I get data from existing
Page 13 and 14: Two RAINFOR-GEM hectare plots in th
Page 15 and 16: 1.2 Setting out the plotSubplot cor
Page 17 and 18: An example surveying path (cf. Cond
Page 19 and 20: Required for jobs that can be sched
Page 21 and 22: Tree #487 in Fragment E plot where
Page 23 and 24: 1.4 Tagging small trees*** Note tha
Page 25 and 26: does, measure underneath the lean o
Page 27 and 28: Measuring a liana at three points i
Page 29 and 30: BOX 2: Five unusual trees.Example 1
Page 31 and 32: Example 3: Here is a large liana on
Page 33 and 34: 1.5 Tree heightTree height should b
Page 35 and 36: 1.6 Sketch map of the plotAt this p
Page 37 and 38: 2. BELOW GROUND MEASUREMENTSWe star
Page 39 and 40: Next, insert the cylindrical core i
Page 41 and 42: BOX 3: How to make an in-growth cor
Page 43 and 44: 2.2 RhizotronsRoot observation pits
Page 45 and 46: sure that it covers the hole but no
Page 47: An example rhizotron transparency f
Page 51 and 52: A metallic rhizotron as in use at t
Page 53 and 54: 2.3 Coarse rootsNeither in-growth c
Page 55 and 56: 3. CO 2 EFFLUX3.1 Total soil CO 2 e
Page 57 and 58: 3.2 Stem CO 2 effluxFirst, choose t
Page 59 and 60: e related to stem growth) to check
Page 61 and 62: Equipment: IRGA and SRC-1, PVC coll
Page 63 and 64: Control No litter Double litterNo e
Page 65 and 66: the S3 hole. Finally, discard the l
Page 67 and 68: 3.4 Coarse woody debris (CWD) CO 2
Page 69 and 70: 4.2 Coarse woody debris (CWD) surve
Page 71 and 72: all of the large, marked wood piece
Page 73 and 74: Equipment: 25 litter traps per ha (
Page 75 and 76: this select the straight line drawi
Page 77 and 78: http://www.eci.ox.ac.uk/research/ec
Page 79 and 80: • The tripod is level so that the
Page 81 and 82: 8. WEATHER AND CLIMATELocal climate
Page 83 and 84: Luiz Aragão repairing an understor
Page 85 and 86: 10. DATA ENTRY AND SERVER UPLOADA c
Page 87 and 88: APPENDIX I: RAINFOR-GEM Fieldwork c
Page 89 and 90: APPENDIX II: How to use an EGM-4 In
Page 91 and 92: • Remember to change the switch i
Page 93 and 94: CO 2 efflux calculationsWhile the E
Page 95 and 96: The volume V airspace (stippled) be
Page 97 and 98: CO 2 flux measurements with EGM: A
Page 99 and 100:
Downloading dataEGM memory can stor
Page 101 and 102:
APPENDIX III: Possible schedule of
Page 103 and 104:
Gerwing JJ, Schnitzer SA, Burnham R
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