Vitalijs Radenkovs, Dace KlavaPHYSICAL - CHEMICAL CHARACTERIZATION<strong>OF</strong> INDUSTRIAL WHEAT BRAN FROM <strong>LATVIA</strong>TA, °14.012.010.08.06.04.02.00.012.059.3510.406.40LSR SSR SSD LSDFigure 4. Titratable acidity of wheat bran samples.particles were similar (1.6. – 2.0 mm), but colourL* 53.25±2.71 (SSR), L* 58.42±1.51 (LSR) wasdifferent.3. Titratable acidity of wheat bran is significantlyinfluenced by technological process of milling,since the milling process highly influences theproportions of the different cell types in the ‘bran’,therefore SC ‘Dobeles dzirnavnieks’ samplescontained the highest titratable acidity 10.40±0.28– 12.05±0.21, whereas SC ‘Rigas dzirnavnieks’sample had 6.40±0.71 to 9.35±0.49.AcknowledgementsThis research has been prepared within the StateResearch Programme ‘Sustainable use of localresources (earth, food, and transport) – new productsand technologies (NatRes)’ (2010.-2013.) Project no.3. ‘Sustainable use of local agricultural resources fordevelopment of high nutritive value food products(Food)’.The authors thank SC ‘Rigas dzirnavnieks’ and SC‘Dobeles dzirnavnieks’ for wheat bran samples.References1. Afaf K.E., Lærke H.N., Knudsen K.E.B., Lampi A.L., Piironen V., Adlercreutz H., Katina K., Poutanen K.,Mand P.A. (2009) Physical, microscopic and chemical characterization of industrial rye and wheat bransfrom the Nordic countries. Food and Nutrition Research, p. 11.2. Camacho J.L.M., Vara L.G., Hamabata A., Escobedo R.M., Salinas V.C. (2004) A pH-stating mechanismin isolated wheat (Triticum aestivum) aleurone layers involves malic acid transport. Journal of PlantPhysiology, 161, pp. 1289–1298.3. Evers A.D., Reed M. (1988) Some novel observations by scanning electron microscopy on the seed coatand nucellus of the mature wheat grain. Cereal Chemistry, 65, pp. 81–85.4. Gottfried S., Hans S. (2000) Handbuch Sauerteig (Handbook of sourdough) In: Gottfried S., Hans S. (eds)pH-Wert und Säuregrad, Klaus Kühn Fotosatz, 2000 Hamburg 70, p. 468.5. Harris P.J., Chavan R.R., Ferguson L.R. (2005) Production and characterisation of two wheat-bran fractionsan aleurone-rich and a pericarp-rich fraction. Molecular Nutrition and Food Research, 49, pp. 536–545.6. Hemery M., Rouau X., Dragan C., Bilici M., Beleca R., Dascalescu L. (2009) Electrostatic propertiesof wheat bran and its constitutive layers: Influence of particle size, composition, and moisture content.Journal of Food Engineering, 99, pp. 114–124.7. Hemery M., Mabille F., Milena R., Rouau M.X. (2010) Influence of water content and negative temperatureson the mechanical properties of wheat bran and its constitutive layers. Journal of Food Engineering, 98,pp. 360–369.8. Khan K., Shewry P.R. (2009) Wheat kernel structure In: Khan K., Shewry P.R. (eds) Wheat chemistry andtehnology, AACC International, St. Paul, Minnesota, pp. 81–145.9. Nermin B., Adem E., Emine N.H., Selman T., Nilgun E., Şenol I. (2006) Effect of wheat germ/bran additionon the chemical, nutritional and sensory quality of tarhana, a fermented wheat flour-yoghurt product.Journal of Food Engineering, 77, p. 683.10. Конева С.И., Могучева Э.П. (2011) Исследование влияния пшеничных отрубей на качество хлебаповышенной пищевой ценности (Research of wheat bran’s influence on quality of bread with highnutritional value). Ползуновский вестник, № 3/2, 2, с. 141–144 (in Russian).Research for Rural Development 2012159
FOOD SCIENCESTHE INFLUENCE <strong>OF</strong> DIFFERENT SELENIUM CONCENTRATIONS ON THE BARLEYGRAIN ´CLASS´ SPROUTING ACTIVITY AND CONTENT <strong>OF</strong> TOTAL PHENOLSKristīna Antoņenko, Viesturs KreicbergsLatvia University of Agriculturee-mail: antokrist@inbox.lvAbstractBarley (Hordeum vulgare L.) grain is the main raw material in brewing industry, because beer taste and productionprocess depend on barley grain qualitative indices. One of the barley grain’s qualitative indices is grain sproutingactivity. There have been numerous researches that showed the effects of selenium (Se) on hull-less barley sproutingactivity and positive influence on biologically active substances and high vitamins concentration in grain (Dūma et al.,2002), but hull-less barley is not widely used. The aim of this study was to explore the influence of different seleniumconcentrations on the barley grains sprouting activity and content of total phenols. Barley with grain viability of 92%have been soaked in diverse solutions (Se concentration 0.5 mg L -1 – 10 mg L -1 ) for 12 hours, then the grain sproutedat the temperature of +18±2 °C for 5 days and dried in the oven for 48 hours at a temperature of +50 °C. Aftersprouting, the sprouting activity of the grain was determined, but total phenols amount was determined after graindesiccation. The obtained results showed that the increase in selenium concentration in a solution increased barleygrain sprouting activity from 16.3% (0.5 mg L -1 ) to 27.9% (10 mg L -1 ), but the amount of total phenols decreased.Key words: barley, sprouting activity, total phenols, selenium.IntroductionBarley (Hordeum vulgare L.) is consumed aroundthe world mostly in the malted form in brewing andbakery industry. In recent years barley has gainedpopularity due to the functional properties of itsbioactive compounds such as β-glucan, arabinoxylan,oligosaccharides, tocols and phenolic compounds(Sharma and Gujral, 2010).Malt is germinated, dried and milled cereal grains.Barley malting is the most widely known controlledgermination process, used to produce malt for brewingpurposes. The types of beer determine such indices asthe taste, aroma and flavor of malt (Kleinwächter et al.,2012; Katina et al., 2007). Therefore malt productionis very important in brewing industry. Malt receives itsown properties exactly during the sprouting process,therefore the quality of malt depends on the qualityof grain.The micronutrient selenium is needed for normalfunctioning of each living cell, because cells structureand life processes depend on micronutrient operation.Selenium is found in the intercellular fluid and itregulats composition and functionality of the cell(Рисман, 1998; Lawrence et al., 2001; Baltess, 1998).It is part of some enzymes and hormones, interactswith vitamins, participates in oxidizing processes andin metabolism of proteins, carbohydrates and fats.Selenium is part of enzyme glutathione peroxidase,the main part of antioxidative defence system in livingcells (Dūma, 2010). Selenium is an important nutrientfor the maintenance of normal cells and tissues in thehuman body. Increasing the amount of selenium infoods is one of the solutions how to solve its deficiencyin nutrition. The most important sources of seleniumin diets are meat, fish and cereals. Cereals enrichedwith selenium are a good possibility for developmentof new food products, especially fermented grainproducts, in this area (Lintshinger et al., 2000; Dūmaet al., 2002).Se content in food and beverages variesgeographically both within and between countries.The Se content in animal products reflects the Selevels in their consumed diet, whereas the Se contentof plants is directly affected by Se levels in the soilin which they are grown. The Recommended DietaryAllowance (RDA) for Se for both men and women is55 μg day -1 (0.7 μmol day -1 ). This recommendation isbased on the amount needed to maximize synthesisof the selenoprotein glutathione peroxidase (GPx),as assessed by the plateau in the activity of theplasma isoform of this enzyme (Navarro-Alarcon andCabrera-Vique, 2008).However, there is still lack of scientific studieson the effect of selenium added to the grain. The aimof this research was to investigate the influence ofdifferent selenium concentrations on the barley grainsprouting activity and content of total phenols.Materials and MethodsPlant materialThe research object was barley grain (variety´Class´) from Ltd. ´Tērvete´, harvested in 2011. Barleygrain were soaked and germinated at room temperature+17±2 °C, under natural day/night conditions usingsodium selenate Na 2SeO 4solutions. The period ofgermination was 120 h. The concentration of seleniumwas 0.5, 1, 3, 5, and 10 mg L -1 . The grain (100 pieces)was soaked for 12 h in the above mentioned solutions(500 mL), and let to sprout. During germination, thesprouts were rinsed four times every day with 100 ml160 Research for Rural Development 2012