ISBN: 978-83-60043-10-3 - eurobic9

Eurobic9, 2-6 September, 2008, Wrocław, Poland
P114. Complexes of Copper(II) with Nicotinamide Adenine Dinucleotide
(NAD + ) in Binary and Ternary Systems Including Spermine (Spm)
L. Lomozik, A. Gasowska, K. Basinski
Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznan, Poland
e-mail: basinski@amu.edu.pl
Nicotinamide adenine dinucleotide (NAD + ) is a coenzyme found in all living cells which consists of two
nucleotides linked through their phosphate groups. In metabolism, NAD + is involved in redox reactions of
cellular respiration, carrying electrons from one reaction to another [1, 2]. The bioligand makes an interesting
object of research because of its biochemical reactivity with the enzymes from the group of dehydrogenases [3].
Unfortunately not much literature on the coordination of NAD + through metals present in living organism has
been published as yet [4].
Interaction of nicotinamide adenine dinucleotide with copper(II) ions in binary and ternary systems including
spermine has been observed. Computer analysis of potentiometric titration data in the Cu(II)-NAD + system has
shown the formation of CuH2(NAD + ), CuH(NAD + ), Cu(NAD + )(OH) and Cu(NAD + )(OH)2 complexes and in the
ternary system including spermine (1, 12-diamino-4, 9-diazadodecane) – the formation of Cu(NAD + )H4(Spm)
and Cu(NAD + )H5(Spm) complexes. To determine the coordination centres, Vis spectra of the complexes were
made. For example, the λmax values obtained were 808.0, 793.7 and 665.0 nm for CuH2(NAD + ), CuH(NAD + ) and
Cu(NAD + )(OH)2, respectively, which corresponds to the {O} type of metallation in CuH2(NAD + ) and
CuH(NAD + ) and the {N, O} type of metallation in Cu(NAD + )(OH)2 complex [5]. Analysis of the 13 C and 31 P
NMR spectra has confirmed these conclusions.
Full recognition of the character of copper-NAD + and copper-NAD + -spermine interactions in model systems is
expected to give information on their probable participation in biological processes, in which nicotinamide
adenine dinucleotide takes part. The results of these observations should yield better insight into intracellular
mechanisms and emphasise the role of metals in living organisms.
References:
[1] S. Sivaranam et al., Biochem., 42, 4406-4413 (2003)
[2] J. M. Berg, J. L. Tymoczko, L. Stryer, Biochemistry, 465-514 (2005)
[3] G. R. Stockwell, J. M. Thornton, J. Mol. Biol., 356, 928-944 (2006)
[4] L. A. Herrero et al., J. Biol. Inorg. Chem., 7, 313-317 (2002)
[5] A. Gasowska, J. Inorg. Biochem., 99, 1698-1707 (2005)
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