ISBN: 978-83-60043-10-3 - eurobic9

Eurobic9, 2-6 September, 2008, Wrocław, Poland
P187. Copper(I) Iodide Complexes with Aromatic Diimines and Aliphatic
Aminophosphines: Characterization, Structures, Antibacterial Properties
and Study of Interaction with pUC18 Plasmid and Bovine Serum Albumin.
R. Starosta a , M. Florek b , J. Król b , W. Barszczewski c , M. Puchalska a , A. Kochel a
a
Faculty of Chemistry, University of Wroclaw, ul. F. Joliot-Curie 14, 50-383 Wroclaw, Poland
e-mail: sta@wchuwr.pl
b
Department of Veterinary Microbiology, Wroclaw University of Environmental and Life Sciences, ul. Norwida
31, 50-375 Wroclaw, Poland
c
Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life
Sciences, ul. Norwida 25, 50-375 Wroclaw, Poland
A large number of copper(I) complexes with tertiary phosphines and tertiary diphosphines have been studied for
their tumoricidal properties [1]. Phosphane copper(I) complexes with 1, 10-phenanthroline and its derivatives,
have been extensively studied mainly for their photophysical properties [2], but have not been investigated for
their potential biological activity to the best of our knowledge.
In this work we present novel copper(I) iodide complexes with aliphatic aminophosphines of general formula:
CuI(NN)P(CH2R)3, where NN = 2, 2’-bpy or 1, 10-phen and P(CH2R)3 = P(CH2-N(CH2CH2)2O)3, P(CH2-
N(CH2CH2)2N-CH3)3 or P(CH2-N(CH2CH2)2N-CH2CH3)3.
All CuI(NN)P(CH2R)3 complexes were prepared in direct
reactions of CuI with diimine and phosphine in 1:1:1 molar ratios
at room temperature. The obtained compounds were characterized
using spectroscopic and crystallographic methods.
Cu(I) has distorted tetrahedral coordination in all investigated
complexes. NMR data indicate that imine ligands are bound to
copper atom symmetrically. The influence of phosphines
coordination to Cu(I) atom on the phosphine part of the
CuI(1, 10-phen)P(CH2-N(CH2CH2)2O)3
1 H NMR
spectra suggest that the phosphorus atoms in phosphine ligands
coordinate more strongly in the complexes with 1, 10-phen than in
the complexes with 2, 2’-bpy.
Presented complexes were screened for their in vitro antibacterial
activity against Gram-negative Escherichia coli and Pseudomonas
aeruginosa and Gram-positive Staphylococcus aureus strains, and
for in vitro antifungal activity against Candida albicans. All the
compounds show significant antibacterial activity against
Staphylococcus aureus.
Investigations of interactions of CuI(1, 10-phen)PR3 and CuI(2, 2’-bpy)PR3 complexes with bovine serum
albumin showed that the diminution of BSA fluorescence signal is stronger for 1, 10-phenanthroline than for 2,
2’-bipyridine derivatives. A circular dichroism study of interactions of the presented complexes with BSA gave
similar results: the decrease of the negative bands characteristic for α-helical structure caused by interactions
with 1, 10-phenanthroline derivatives is bigger than with 2, 2’-bipyridine derivatives.
The agarose gel electrophoresis studies have been carried out and it was found that the investigated complexes
interact with pUC18 plasmid and break the DNA supercoiled to nicked and linear DNA forms.
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