Analyse avec le logiciel imagej d'un lot d'images en microscopie par ...

C:\Documents and Settings\montarou\Bureau\correction\chandez\PLU_RGB_Avg_v3_.java jeudi 22 juillet 2010 16:22
in2p3-00530281, version 1 - 28 Oct 2010
}
/*
r = new byte[width*height];
g = new byte[width*height];
b = new byte[width*height];
cp = (ColorProcessor)is.getProcessor(1);
cp.getRGB(r,g,b);
//rgbStack.deleteSlice(1); // delete 'imp' stack
rgbStack.addSlice(null,r);
rgbStack.addSlice(null,g);
rgbStack.addSlice(null,b);
pw.println("Separating channels and creating RGB mask for " + imp.getTitle());
pw.println("rgb stack size= "+rgbStack.getSize());
// Return an 'ImageStack' rgbStack with separating channels
return rgbStack;
Apply correction on scienceImg with PLUredmask, PLUgreenmask and PLUbluemask)
For each channel, multiply science image by PLU/mean
Creates a ImageStack("red"+"green"+"blue")
Converts this Stack to RGB
Image/Color/Stack to RGB
Cree une stack avec les canaux R, G, B corriges
Converts a 2 or 3-slice stack to an RGB image assuming that the slices are in R, G, B order.
Equivalence Menu:
"Image/Color/Channels Tool.../More>>/Convert to RGB"
Equivalence Commande:
IJ.doCommand("Stack to RGB"); // The stack must be 8-bit or 16-bit grayscale.
*/
void applyCorrection(ImagePlus scienceImg) {
if (rgbPLUmask[0] == null || rgbPLUmask[1]== null|| rgbPLUmask[2]== null) {
IJ.showMessage("Chargement de l'image", "Impossible");
return;
}
// Splits the specified RGB image or stack into three 8-bit grayscale images or stacks.
RGBStackSplitter s = new RGBStackSplitter();
s.split(scienceImg.getStack(), true);
// Get filename without Extension
int index = scienceImg.getTitle().lastIndexOf('.');
String title = scienceImg.getTitle().substring(0, index);
ImagePlus scienceRedChannel = new ImagePlus(title + " (Red)", s.red);
ImagePlus scienceGreenChannel = new ImagePlus(title + " (Green)", s.green);
ImagePlus scienceBlueChannel = new ImagePlus(title + " (Blue)", s.blue);
if (debug==1) {
String fileName = null;
//scienceRedChannel.show();
fileName = outputDir+"splitter "+scienceRedChannel.getTitle()+".tif";
new FileSaver(scienceRedChannel).saveAsTiff(fileName);
//scienceGreenChannel.show();
fileName = outputDir+"splitter "+scienceGreenChannel.getTitle()+".tif";
new FileSaver(scienceGreenChannel).saveAsTiff(fileName);
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