Analyse avec le logiciel imagej d'un lot d'images en microscopie par ...

C:\Documents and Settings\montarou\Bureau\correction\chandez\PLU_RGB_Avg_v3_.java jeudi 22 juillet 2010 16:22
blueStack.addSlice("blueConvertToByte", correctedBlueChannel.getProcessor());
// do stack conversions
RGBStackMerge stackMerge = new RGBStackMerge();
int d = redStack.getSize();
boolean keep = true;
ImageStack stackRGB = stackMerge.mergeStacks(width, height, d, redStack, greenStack,
blueStack, keep);
ImagePlus correctRGBVersion = new ImagePlus("RGB result of "+scienceImg.getShortTitle
(), stackRGB);
new FileSaver(correctRGBVersion).saveAsTiff(outputDir+"RGB result of "+scienceImg.
getShortTitle()+" (scale="+(doScaling?"true":"false")+").tif");
correctRGBVersion.show();
in2p3-00530281, version 1 - 28 Oct 2010
}
/*
IJ.run("Histogram");
Do multiplication/division/addition/sousytaction of two images
Call on each RVB channel
Creates a "Corrected_" image
*/
public static ImagePlus doCorrection(ImagePlus imp1, ImagePlus imp2) {
ImageProcessor ip1 = imp1.getProcessor(); // target processor ip1
ImageProcessor ip2 = imp2.getProcessor(); // source processor ip2
// 8-bit image contains pixels which values range from 0 to 255
// If you have a pixel with a value of 200 and you multiply it by 3 it will become 255 not 600.
// So, 32 bits conversion!
// Take original image transform it into 32-bit (Image > Type > 32-bit)
// Mathematic operation should not have under/overflow problems
ip1 = ip1.convertToFloat();
ip2 = ip2.convertToFloat();
ip1 = ip1.duplicate(); // Returns a duplicate of this image.
try {
if (ip1 instanceof ColorProcessor)
IJ.showMessage("calculate", "Error : ip must be converted to float");
switch (operator) {
case 0: operator = Blitter.ADD; break;
case 1: operator = Blitter.SUBTRACT; break;
case 2: operator = Blitter.MULTIPLY; break;
case 3: operator = Blitter.DIVIDE; break;
}
// Performs correction using specified method
ip1.copyBits(ip2, 0, 0, operator);
}
catch (IllegalArgumentException e) {
IJ.error("My error " + imp1.getTitle() + ": " + e.getMessage());
return null;
}
ImagePlus result = new ImagePlus("Corrected_32-bit_"+imp1.getTitle(), ip1);
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