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com as técnicas de FISH, CMA, DAPI - IAC

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1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

4.2.6 Medid<strong>as</strong> cromossômic<strong>as</strong><br />

As medid<strong>as</strong> dos cromossomos da espécie A. inodora, realizad<strong>as</strong> em célul<strong>as</strong><br />

submetid<strong>as</strong> às diferentes técnic<strong>as</strong> <strong>de</strong> coloração, variou <strong>de</strong> 8,89 ± 1,12 µm a 29,85 ± 4,76<br />

µm (Tabela 4). Todos os pares cromossômicos apresentaram coeficiente <strong>de</strong> variação<br />

menor que 20%. O <strong>com</strong>primento total da cromatina (CTC) para esta espécie foi <strong>de</strong><br />

220,97 ± 28,64 µm e o índice <strong>de</strong> <strong>as</strong>simetria TF% <strong>de</strong> 22,48% (Tabela 8).<br />

A<br />

C<br />

1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

<strong>CMA</strong>3/DA<br />

<strong>DAPI</strong>/AMD<br />

<strong>FISH</strong> - pTa71 - 45S<br />

<strong>FISH</strong> - pScT7 - 5S<br />

Figura 9 - Alstroemeria inodora – I<strong>de</strong>ogram<strong>as</strong> representativos. (A) Bandamento <strong>com</strong><br />

<strong>CMA</strong>3/DA; (B) Bandamento <strong>com</strong> <strong>DAPI</strong>/AMD; (C) Sinais <strong>de</strong> hibridação in situ para<br />

sequênci<strong>as</strong> <strong>de</strong> rDNA 45S <strong>com</strong> a sonda pTa71; (D) Sinais <strong>de</strong> sequênci<strong>as</strong> <strong>de</strong> rDNA 5S<br />

<strong>com</strong> a sonda pScT7; (E) Representação do conjunto <strong>de</strong> resultados d<strong>as</strong> técnic<strong>as</strong><br />

<strong>CMA</strong>3/DA, <strong>DAPI</strong>/AMD e hibridação in situ para rDNA 45S e 5S.<br />

E<br />

B<br />

D<br />

43

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