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com as técnicas de FISH, CMA, DAPI - IAC

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(Figura 15C). Estes resultados <strong>de</strong> bandamento NOR na espécie A. psittacina são<br />

apresentados pela primeira vez no presente trabalho.<br />

1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

4.4.6 Medid<strong>as</strong> cromossômic<strong>as</strong><br />

A<br />

C<br />

1 2 3 4 5 6 7 8<br />

1 2 3 4 5 6 7 8<br />

<strong>CMA</strong>3/DA<br />

<strong>DAPI</strong>/AMD<br />

<strong>FISH</strong> - pTa71 - 45S<br />

<strong>FISH</strong> - pScT7 - 5S<br />

Figura 15 - Alstroemeria psittacina – I<strong>de</strong>ogram<strong>as</strong> representativos. (A) Bandamento<br />

<strong>com</strong> <strong>CMA</strong>3/DA; (B) Bandamento <strong>com</strong> <strong>DAPI</strong>/AMD; (C) Sinais <strong>de</strong> hibridação in situ<br />

para sequênci<strong>as</strong> <strong>de</strong> rDNA 45S <strong>com</strong> a sonda pTa71; (D) Sinais <strong>de</strong> sequênci<strong>as</strong> <strong>de</strong><br />

rDNA 5S <strong>com</strong> a sonda pScT7; (E) Representação do conjunto <strong>de</strong> resultados d<strong>as</strong><br />

técnic<strong>as</strong> <strong>CMA</strong>3/DA, <strong>DAPI</strong>/AMD e hibridação in situ para rDNA 45S e 5S.<br />

As medid<strong>as</strong> obtid<strong>as</strong> para os <strong>com</strong>primentos totais dos cromossomos da espécie A.<br />

psittacina variaram <strong>de</strong> 8,98 ± 0,23 µm a 28,45 ± 0,60 µm e todos os pares apresentaram<br />

um coeficiente <strong>de</strong> variação menor que 20% (Tabela 7). O <strong>com</strong>primento total da<br />

E<br />

B<br />

D<br />

53

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