livro

Agrárias INCT – Institutos Nacionais de Ciência e Tecnologia
INCT – Institutos Nacionais de Ciência e Tecnologia
Agrárias
Associated Institutions
UFLA; UFV; EPAMIG; IAPAR; Embrapa – Recursos Genéticos e Biotecnologia;
Embrapa – Café; INCAPER; IAC
Main researchers
Gladyston R. Carvalho, Laércio Zambolim, Carlos A. Colombo, Alan Carvalho
Andrade, Édila Vilela de R. Von Pinho, Flávio Meira Borém, Renato
Paiva, Carlos Henrique S. de Carvalho, Luciano Vilela Paiva, Sara Maria
Chalfoun, Paulo Rebelles Reis, Luiz Roberto G. Guilherme, Moacir de S.
Dias Júnior, Antônio E. Furtini Neto, Luiz Gonzaga de C. Júnior.
Main research topics
The INCT of Coffee has 15 lines of research, most of them focused on
biotechnology. Research Lines 1 - Breeding for resistance to abiotic
stress and beverage quality; 2 - Characterization of genes in the interaction
with rust; 3 - Physical and genetic mapping of QTL regions used in
assisted selection and cloning, 4 - QTL for drought tolerance and quality
of beverage; 5 - Genetic evaluation of seed quality; 6 - Phenological,
biochemical, metabolomic and proteomic analyses related to the quality
of the beverage; 7 - Cryopreservation for coffee explants; 8 - Production
of plantlets by somatic embryogenesis; 9 - Genetic transformation; 10
- Induction of resistance against diseases / beverage quality; 11 - Integrated
management of major pests; 12 - Dynamics and fate of pesticides
and heavy metals in soil, 13 - Properties of soils under systems of weed
control; 14 - Differentiated management of phosphorus for the crop; 15 -
Technological platform for coffee commercialization.
4. CRUZ, F. et al. Evaluation of coffee reference genes for relative expression
studies by quantitative real-time RT-PCR. Mol. Breed 23:607-616, 2009.
5. MISSIO, R.F. et al. Development and validation of SSR markers for
Coffea arabica L. Crop Breed Appl Biotechnol 9:361-371, 2009.
6. MATOS, F.S. et al. Phenotypic plasticity in response to light in the coffee
tree. Environmental and Experimental Botany 67:421-427, 2009.
6. TEODORO, A.V. Agroforestry management affects coffee pests contingent
on season and developmental stage. Agricultural and Forestry
Entomology 11:295-300, 2009.
Contacts
INCT-Café / Mário Lúcio Vilela de Resende
Universidade Federal de Lavras (UFLA), Campus UFLA
CP 3037, CEP: 37200-000
Tel: (35) 3829-1793 / 3829-1015
E-mail: inctcafe@ufla.br / mlucio@dfp.ufla.br
http://www.cnpq.br/programas/inct/_apresentacao/inct_cafe.html
Flávio M. Dantas, Benito Soto-Blanco, Cacilda Borges do Valle, Carla Lopes
Mendonça, Carlos H. Tokarnia, Caroline Argenta Pescador., Claudio Estevão
Farias da Cruz, David Driemeier, Edson Moleta Colodel, Everton Ferreira
Lima, Felício Garino Junior, Fernando Castilhos Karam, Fernando Zanotta da
Cruz, Gabriela Riet-Correa, Jael Soares Batista, José Augusto Bastos Afonso
da Silva, José Henrique Pedrosa-Macedo, José Renato Borges, Julianne
Milléo, Luciano Nakasato, Márcia Almeida Melo, Marcio Botelho Castro,
Marcos Barbosa Ferreira, Marcos de Almeida Souza, Maria Clorinda Soares
Fioravanti, Mauro Pereira Soares, Mitsue Haraguchi, Karine Bonucielli
Brum, Paulo Bandarra, Ricardo A. A. Lemos, Silvana Maria Medeiros de
Sousa Silva, Valíria Cerqueira Duarte, Walmir Silva Garcez
Main research topics
• Integrated control methods for Senecio spp including control by insects
and control by sheep associated with agricultural and grazing management
practices.
• Control of Brachiaria spp poisoning by the use of resistant animals and
Brachiaria species or varieties with low saponin concentrations.
• Use of conditioned food induced aversion methods to control plant
poisonings.
• Use of fluoroacetate degrading microorganisms in the rumen of susceptible
livestock to reduce the toxicity of fluoracetate-containing plants.
• Production of a vaccine to prevent the intoxication by ricinin (Ricinus
communis).
• Toxic plants affecting reproduction in sheep and goats.
• Toxic plants for livestock in the Northeastern, Northern and Central-
-Western regions.
equidae in northern Brazil. J Vet Diagn Invest 21:000–000 (2009)
de Souza Lima M.C.J. & Soto-Blanco B. 2010. Poisoning in goats by
Aspidosperma pyrifolium Mart.: Biological and cytotoxic effects. Toxicon
55(2-3):320-324.
Melo GWS et al. 2010. Poisoning of goats by the pods of Luetzelburgia
auriculata. Toxicon 55:1115–1118
Pacífico da Silva I. & Soto-Blanco B. 2010. Conditioning taste aversion
to Mascagnia rigida (Malpighiaceae) in sheep. Research in Veterinary
Science 88(2): 239-241.
Contacts
Universidade Federal de Campina Grande/Hospital Veterinário do Centro
de Saúde e Tecnologia Rural, Campus de Patos/PB
e-mail: franklin.riet@pq.cnpq.br; riet@cstr.ufcg.edu.br
Summary of achievements and perspectives
Three products are under research and development, which will certainly
Summary of achievements and perspectives
add value to the coffee agribusiness. The first refer to the production
of plantlets by somatic embryogenesis, which is considered the most
suitable alternative to the multiplication of superior hybrid plants on a
Experiments with Brachiaria spp poisoning demonstrated that there are
resistant animals to the intoxication, and that resistance is probably
genetic. There are variations in the saponin content between of Brachiaria
Luetzelburgia auriculata. The poisoning by this plant, which occurs in the
state of Piauí, is one of the many new plant poisonings reported by the
INCT in Northeastern, Central Western and Northern Brazil
large scale (Figure 1). Seedlings from plants with characteristics of great
species. Resistant animals and selection of Brachiaria species or varieties
interest such as resistance to leaf miner and rust, good quality beverage
with low saponin content will be used to prevent the intoxication. Three
and high productivity are under multiplication and will be commercially
trials using lithium chloride to avert goats against swainsonine contained
available very soon. The second technology consists on the production
of different formulations based on extracts from coffee leaves and coffee
berry husks, capable of inducing resistance in plants. These formulations
stimulate the defense system against pathogens in various crops. After
Control of powdery mildew (Oidium sp.) in eucalyptus. Plants sprayed
with formulation based on extracts from coffee leaves (1) and water
sprayed control (2)
plants suggest that aversion is efficient to prevent the ingestion of I. carnea
and Turbina cordata in goats under field conditions. Also the use of lithium
chloride was effective to create aversion to Mascagnia rigida. Two trials are
ongoing for the control of Senecio spp. by grazing sheep, and preliminary
National Institute of Science and Technology
Genetic and Health Information of Brazilian
Animal Production
several experiments, in Brazil and abroad, the effectiveness of these
results show that this is a good way of control. Studies on the biology of the
formulations has been proven against fungal and bacterial diseases in
various crops, as detailed in Figures 2, 3 and 4. The third technology National Institute of Science and Technology for
insect Phaedon confinis revealed that it causes serious damage to plants
of Senecio brasiliensis. Normal diet, oviposition, survival and development
Management committee
Rômulo Cerqueira Leite (Coordinator)
under development at the INCT-Coffee, refers to formulations produced
from the fungus Cladosporium cladosporioides, which improves the
the Control of Plant Poisonings
of P. confinis is restricted to S. brasiliensis suggesting its potential for the
biologic control off this plant. Two dehalogenase containing bacteria (Ente-
Concepta Margaret McManus - Pimentel (Vice-coordinator)
Janaína de Paula Coelho
quality of coffee beverage (Figure 5). Basic research is also carried at the
roccus sp and Bacillus sp) that hydrolyze fluoracetate had been isolated. Marc Henry
INCT- Coffee, aiming at characterizing and selecting, phenotypically and Management committee
Experiments to inoculate these or other genetic modified bacteria in the Denise Aparecida Andrade de Oliveira
molecularly, populations of coffee trees with desirable agronomic charac- Franklin Riet-Correa (Coordinator)
rumen of ruminants to control poisoning by fluoracetate-containing plants
teristics, with emphasis on resistance to abiotic and biotic stress and on Rosane Maria Trindade de Madeiros (Vice-coordinator)
will be performed. Experiments in rats demonstrated the teratogenicity Associated Institutions
the quality of the beverage, and at improving the integrated management Ana Lúcia Schild
of Aspidosperma pyrifolium and Mimosa ophtalmocentra. Experiments in UFMG; UnB; UFPR; UFV; USP; UFPA; UEPG; UEL; UNESP; UFLA; UFRP;
of key coffee pests and diseases.
Gabriela Riet-Correa
goats demonstrated that Mimosa tenuiflora, a well known cause of malfor- UFG; UFF; PUC-MG; EPAMIG; LANAGRO-MG; MAPA
Marcio B. Castro
mations also causes embryonic deaths. Ten new plant poisonings had been
Main publications
reported from Central-Western, Northeastern and Northern Brazil. Main researchers
1. OLIVEIRA, R.R. et al. In silico and quantitative analyses of MADS-Box Associated Institutions
Romário Cerqueira Leite, João Paulo Haddad, Denise Aparecida
genes in Coffea arabica. Plant Mol Biol Rep 28:460-472, 2010.
UFCG, UFPel, UFERSA, UFRPE, UFRRJ, UFMT, UFMS, UFG, UFRGS, UFPA,
Main publications
Andrade de Oliveira, Helder Louvandini, Hélio Vilela Barbosa Júnior, Ana
2. MEDEIROS, F.C.L. et al. Defense gene expression induced by a coffee- UnB, Laboratório de Pesquisas Veterinárias Desidério Finamor, EMBRAPA,
Almeida MB et al. 2009. Conditioned aversion in sheep induced by Bac- Cláudia Pinto Cottorelllo, Cristiano Barros de Melo, Nelson Rodrigues
-leaf extract formulation in tomato. Physiological and Molecular Plant UEAM, UFPR, UEPG, UFPI, Instituto Biológico de São Paulo
charis coridifolia. App. An. Behav. Scien, 117: 197-200, 2009.
da Silva Martins, Concepta Margareth McManus Pimentel, Martinho
Pathology 74:175-183, 2009.
Anjos BL et al. Poisoning of sheep by seeds of Crotalaria retusa: Acquired de Almeida e Silva, Vera Fernanda Martins Hossepian de Lima, Daniel
3. NOBILE, P.M. et al. Transcriptional profile of genes involved in the biosyn- Main researchers
resistance by continuous administration of low doses. Toxicon 55: 28-32. Crepaldi, Marc Henry, Andrea Micke Moreno, Otávio Mitio Ohashi,
thesis of phytate and ferritin in Coffea. J Agric Food Chem 58:3479-87, 2010. Franklin Riet-Correa, Rosane M. T. Medeiros, Ana Lucia Schild, Antônio
Cerqueira VD et al.. 2009. Colic caused by Panicum maximum toxicosis in Josiane Tavares de Abreu
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