livro

Agrárias INCT – Institutos Nacionais de Ciência e Tecnologia
INCT – Institutos Nacionais de Ciência e Tecnologia
Agrárias
Marisa Vieira de Queiroz, Raul Narciso Carvalho Guedes, Renato de Oliveira
Resende, Rogelio Lopes Brandão, Sergio Herminio Brommonschenkel,
Simone da Graça Ribeiro, Wellington Garcia Campos
Main research topics
We are studying the initial signaling mechanisms during plant/microbe
interactions primarily using geminivirus infection both in the model
system Arabidopsis and in crop plants. Our research also focuses on
elucidating the molecular basis for interaction of other detrimental
organisms, such as fungi and insects, with their plant hosts. A deeper
understanding of these interactions during plant infection or infestation
will enable researchers to breed for or engineer superior crops.
2. Lopes et al. 2010. Journal of Insect Physiology (DOI: 10.1016/j.
jinsphys.2010.02.020)
3. Silva et al. 2010. Comparative Biochemistry and Physiology B 155: 12-19.
4. Aragão FJ, Faria JC. 2009. Nature Biotechnology 2009 27(12):1086-8.
5. Santos et al. 2009. PLoS ONE 4, e5781. doi:10.1371/journal.
pone.0005781
6. Alves Junior et al. 2009. Virology 387, 257-266. doi:10.1016/j.
virol.2009.01.046
states and others countries, is to develop scientific and technological background
for identification, synthesis and use of semiochemicals (insects and
plants) in Brazilian agriculture. At same time the Institute intends to contribute
to the regional balance of this research area in Brazil, with emphasis
of development of human resources and training of young researchers.
National Institute of Science and Technology of
Tropical Fruits – INCT-FT
Management committee
Narendra Narain (Coordinator)
Sueli Rodrigues (Vice-coordinator)
Edy Sousa de Brito
Fabiano André Narciso Fernandes
Gustavo Adolfo Saavedra Pinto
Jane de Jesus da Silveira Moreira
Contacts
Summary of achievements and perspectives
The results obtained by the INCT of Semiochemicals in Agriculture during
the first year of activities should result in solutions for problems which afflict
Brazilian agribusiness. An example which could be cited as an example is
BIOAGRO/Universidade Federal de Viçosa
the case of ‘greening’ in citrus, where research developed with its vector, the
36570.000, Viçosa, MG, Brazil
psyllid, Diaphorina citri, show that the volatiles studied in Brazil can solve
e-mail: bbfontes@ufv.br, bethfontes@funarbe.org.br, bethfontes@
this problem which threatens to destroy the Brazilian citrus industry. This Associated Institutions
Summary of achievements and perspectives
pq.cnpq.br
research is also about to result in a patent in collaboration with Fundecitrus UFS; UFC; EMBRAPA-CNPAT
With respect to the major topic of geminivirus-host interactions we have Fone: +55-31-38992949; +55-31-38997320
and the Valencian Institute of Agrarian Research from Spain. Also, the results
achieved relevant progresses in understanding the mechanisms of virus Fax: +55-31-38992864
obtained for the sugarcane borer, Diatraea saccharalis, which have the aim Main researchers
intracellular movement, diversity of begomovirus in the Brazilian territory http://www.inctipp.net
of identifying and synthesizing a sexual pheromone for this pest for use is Elma Regina Silva de Andrade, Jane de Jesus da Silveira Moreira, Marcelo
and the molecular bases by which geminivirus subvert the defense stra-
biological control for its control, demonstrates the advance of the research. Augusto Gutierrez Carnelossi, Maria Aparecida Azevedo Pereira da Silva,
tegies of plant hosts. Regarding geminivirus-acquired resistance our team
However, among the objectives of the INCT of Agricultural Semiochemicals, Mércia de Sousa Galvão, Narendra Narain, Fabiano André Narciso Fer-
was the first one to demonstrate geminivirus immunity and obtain the first
the formation of a network and highly-trained human resources, can also nandes, José Maria Correia da Costa, Maria Isabel Gallão, Maria Raquel
transgenic geminivirus-resistant plant in the field. We are now in position
contribute to the maintenance of quality and advances in Brazilian science. Alcântara de Miranda, Sueli Rodrigues, Carlos Farley Herbster Moura,
to transfer the resistance molecular strategy to other relevant crops.
Deborah dos Santos Garruti, Ebenézer de Oliveira Silva, Edy Sousa de
By using genomic information, we have also identified more than fifty genes
Main publications
Brito, Gustavo Adolfo Saavedra Pinto, Henriette Monteiro Cordeiro de
that encode proteins (effectors) secreted by rust fungi during compatible
Nava, D.E.; Gomez-Torres, M.L.; Rodrigues, M.D.; Bento, J.M.S.; Haddad, Azeredo, Ricardo Elesbão Alves
interactions with eucalyptus, coffee and soybean. Agrobacterium-mediated
M.L.; Parra, J.R.P. 2010. The effects of host, geographic origin, and gender
transient assays are now being conducted to identify proteins that are
on the thermal requirements of Diaphorina citri (Hemiptera: Psyllidae). Main research topics
secreted into the plant cell and recognized by coffee, eucalyptus and soybean
Environmental Entomology, 39(2):678-684.
• Biotechnological Processes (enzyme maceration, probiotics, prebiotics
resistance proteins. This knowledge will allow us to identify natural resistance
Bergmann, J.; Gonzales, A.; Zarbin, P.H.G. 2009. Insect Pheromone Research obtained by enzymatic synthesis)
genes that are able to provide broad spectrum and durable rust resistance,
in South America. Journal of the Brazilian Chemical Society, 20: 1206-1219. • Drying Technology (ultrasound as pre-treatment for drying of fruits)
and to test if we can obtain broad spectrum rust resistance by expressing in
Lima, Eraldo R.; McNeil, Jeremy N. 2009. Female sex pheromones in • Post-harvest Physiology (Analysis and Minimal Processing)
planta RNAi constructs targeting conserved effectors.
the host races and hybrids of the fall armyworm, Spodoptera frugiperda • Development of Fruit-based Edible Films and Coatings
With respect to plant-insect interaction studies, complementary assays
(Lepidoptera: Noctuidae). Chemoecology, 19: 29-36.
• Food Analysis (phenolic compounds, antioxidants, vitamins and others)
carried out in our laboratories confirm the prelavence of the lypoxyenase
Parra-Pedrazzoli, A.L.; Leal, W.S.; Vilela, E.F.; Mendonça, M.C.; Bento, • Sensory Analysis (affective tests, descriptive tests and others)
pathway in the production of protease inhibitors in soybean. Similar
J.M.S. 2009. Synthetic sex pheromone of citrus leafminer in Brazlian • Flavor and Aroma Analysis
response also seems to take place in eucalyptus, but its importance is
citrus groves. Pesquisa Agropecuária Brasileira 44: 676-680.
only secondary. In coffee the situation is more complex because of the
Summary of achievements and perspectives
involvement of volatiles in the insect interaction with the plant. The vel-
Contacts
The first year of INCT-FT activities resulted in a data bank of GC/MS volatile
vetbean caterpillar (Anticarsia gemmatalis) is well-adapted to soybean
ESALQ/USP – Depto de Entomologia e Acarologia
standards; a minimal processing technology applying antioxidants for
and to its proteinase inhibitors, besides synthetic inhibitors. These are
mainly trypsin-like serine-proteinase inhibitors and we had the opportunity
to verify that this caterpillar also express cysteine-preoteinases,
Above: Phakopsora pachyrhizi (soybean rust); below: Anticarsia
gemmatalis (velvetbean caterpillar)
Caixa Postal 09
13418-900 Piracicaba SP
Tel. 19-3429-4199; Fax. 19-3429-4120
mango preservation; the development and optimization of jam and jellies
production from caja-umbu; umbu, mango and siriguela fruits; the development
and optimization of a cashew apple fermented beverage production
which seems to significantly minimize the effect of trypsin-like proteinase
E-mail inctsa@esalq.usp.br
process; process for obtaining cashew apple juice powder by combining
inhibitors. In addition, the microorganism fauna associated with the
Homepage http://www.esalq.usp.br/inctsa
osmotic dehydration and lyophilization; development of edible coating
caterpillar midgut also exhibits proteolytic activity with compensatory
films prepared from mango and acelora pulps, with and without cellulose
potential to trypsin-like inhibitors. We are currently refining the characterization
of serine- and cysteine-proteinases of the velvetbean caterpillar
aiming the design of a proteinase inhibitor suitable to this species. The
National Institute of Science and Technology of
Semiochemicals in Agriculture
nanocrystals; ultrasound assisted dehydration of fruits (pineapple; malay
apple; papaya; sapoti and strawberry). On the biotechnology process research,
probiotic and prebiotic pineapple and melon juices were obtained. The
characterization of physiological and behavioral responses to proteinase
researches under investigation characterize the positive effect of the “foam
inhibitors is also underway for stinkbugs in regular and lipoxygenase- Main researchers
mat” process in dehydration of siriguela; in the development of concentra-
-suppresed soybean seeds. We are also carrying out the same characte- José Roberto Postali Parra (Coordinator), José Maurício S. Bento, Paulo
ted aroma extracts of passion fruit and pineapple and in the determination
rization for the diamondback moth (Plutella xylostella) and the eucalyptus
defoliating caterpillar Thyrinteina arnobia. The characterization of
Henrique G. Zarbin, Antônio Euzébio Goulart Santana, Eraldo Lima
of functional compounds of tropical fruits by LC-DAD-ESI-MS.
proteinases, and also amylases, in seed beetles is also underway to Main research topics
Main publications
assess the possibility of using mimetic proteinase (or amylase) inhibitors The main challenge of INCT of Semiochemicals in Agriculture is to reduce the
- Dehydration of Malay apple (Syzygium malaccense L.) using ultrasound
against these species.
use of agrochemicals, preserving the environment for sustainable agriculture.
as pre-treatment. Food and Bioprocess Technology, v. online, p. 1-6, 2010.
The research topic requires multidisciplinary studies on chemistry of insect
- Drying of Exotic Tropical Fruits: A Comprehensive Review. Food and
Main publications
and plant volatiles, artificial rearing, biology and behavior of insects, as well
Bioprocess Technology, v. online, p. 1-23, 2010;
1. Magalhães et al. 2010. Journal of Economic Entomology (DOI:
as integration with other methods of control for agricultural pests.
- Passion fruit. In: Feng Chen, H. W. Hui, L. M. L. Nollet. (Org.). Hand-
150
10.1603/EC09362)
The aim of the research group, formed by scientists from different Brazilian
Pheromone trap for citrus pest
book of fruit flavors. New York: John Wiley & Sons, 2010
151