4th EucheMs chemistry congress

tuesday, 28-Aug 2012
s601
chem. Listy 106, s587–s1425 (2012)
Analytical chemistry Electrochemistry, Analysis, sample manipulation
separation methods – ii
o - 1 3 1
new fiBerS for SPMe And LC/MSn MonitorinG
of SeLeCted druGS And their MetABoLiteS
B. BuSzewSKi 1
1 Nicolaus Copernicus University F. of Chemistry, Chair of
Environmental Chemistry & Bionanalytics, Torun, Poland
The quantitative and qualitative determination of drugs and
biologically active compound becomes more and more important
in pharmacokinetic analysis and therapeutic drug monitoring. The
most relevant matrices to be analyzed for this purpose are plasma
or blood, due to providing a good correlation between their
concentration and pharmacological effects. One of the major tools
in the pharmacokinetic studies is the combination of
high-performance liquid chromatography and mass spectrometry
(HPLC-MS). This simple and selective method has been used for
the determination of drugs belonging to different medicinal
classes in human plasmaPolypyrrole (PPy) and polythiophene
(PTh) SPME coatings and their derivatives with molecular
template were used and their ability to extract selected drugs from
different classes with different physico–chemical properties and
of widely varying polarities were evaluated. SPME fibers
are manufactured electrochemically by coupling the
homemade electropolymerization system to a new generation
potentiostat/galvanostat in three-electrode array.
Physico-chemical properties, mechanical and chemical stabilities
were characterized by scanning electron microscopy and infrared
spectrometry. The SPME coatings were evaluated by analyzing
clinically relevant antibiotic drugs – linezolid, daptomycin,
amoxicillin and moxifloxacin. Important factors in the
optimization of SPME were discussed. Finally, the
chromatographic measurements were performed by HPLC/MS.
The potential of polymeric SPME sorption coatings for selective
extraction of the drugs was demonstrated considering the results
obtained for the standard solutions and applied to human plasma
samples.
Developed method can be used for the quantitative analysis
of selected biologically active compounds, and provide a potential
application to study the metabolism and pharmacokinetics of other
drugs from different medical classes from the biological matrices.
The results demonstrate the potential of in vivo SPME as a useful
sample preparation tool for chromatographic based metabolomics
drug monitoring in the biomedical application from patients
receiving therapeutic dosages.
Acknowledgments: This work was supported by National
Science Center (Cracow, Poland), no. 2011/01/N/ST4/03178.
Keywords: fibers; spme; drug; metabolites;
separation methods – ii
4 th EucheMs chemistry congress
o - 1 3 2
ChroMAtoGrAPhiC deterMinAtion of
CArBohydrAteS in PLAntS – An overview of
SeverAL AtteMPtS
M. rAeSSLer 1
1 MPI Biogeochemie, SpecLab, Jena, Germany
Carbohydrates and sugar alcohols are important plant
components the composition of which may markedly vary with
season, light availability and status of plant growth. Apart from
plant growth status, carbohydrates also reflect changes of
photosynthetic activity and abiotic stress phenomena, such as
hyperosmosis. In addition to plant physiology, the exact
identification and reliable quantification of carbohydrates in plant
material is also of growing importance for the calculation of more
sophisticated carbon balances of numerous biogeochemical
processes.
Today, liquid chromatography is the most suited method for
the accurate analysis of carbohydrates and sugar alcohols.
Carbohydrates can be subdivided in mono- (e.g. glucose), di- (e.g.
sucrose), oligo- (e.g. raffinose) and polysaccharides (e.g. starch,
inulin, polyfructans). The several components can be detected by
either pulsed amperometry (PA), refrective index (RI), evaporate
light scattering (ELS), or mass spectrometry (MS).
This presentation is based on both the research of our group
[1-3] dealing with the development of analytical methods for the
determination of carbohydrates, sugar alcohols and
polysaccharides in several plant species, and a recent review
article by myself [4].
references:
1. M. Raessler, Proceedings of the 33rd International
Symposium on High Performance Liquid Phase
Separations and Related Techniques (HPLC 2008),
Kyoto, Japan, 101.
2. M. Raessler, B. Wissuwa, A. Breul, W. Unger, T. Grimm,
J. Agric. Food Chem. 56 (2008) 7649.
3. M. Raessler, B. Wissuwa, A. Breul, W. Unger, T. Grimm,
Anal. Methods 2 (2010) 532.
4. M. Raessler, Sample preparation & current applications
of liquid chromatography for the deter-mination of
non-structural carbohydrates (NSC) in plants.
TrAC-Trends in Analytical Chemistry 30 (2011) 1833.
Keywords: Carbohydrates;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc