4th EucheMs chemistry congress

Poster Session 2
s1123
chem. Listy 106, s257–s1425 (2012)
Poster session 2 - Analytical Chemistry
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deterMinAtion of iron (ii), iron (iii) And
totAL iron in SoMe SS-thALASSeMiA PAtientS
uSinG different AnALytiCAL teChniqueS
n. fAKhre 1 , d. ALi 1
1 Univ. of Salahaddin, Chemistry, Erbil, Iraq
Iron as an essential element for living organisms, is oxygen
transport and its role in oxidative-reductive reaction that utilize
its alternative ferrous-ferric states. The biological importance of
iron is widely attributed to its chemical properties, there are two
oxidative forms, ferrous (Fe2+) and ferric (Fe3+) iron. Failure in
this control result in anemia or iron overload. Thalassemia is a
pediatric inherited disease, it is a type of chronic, microcytic
anemia. It is gene-linked disease that can cause serious health
problems because it can lead to the destruction of red blood cells.
There are many well-known analytical methods for determination
of iron (II) and iron (III). A mong these methods: gravimetric,
titrimetric, potentiometric, conductometric, batch and flow
– injection spectrophotometric methods. In the present study, two
batch spectrophotometric, atomic absorption spectrometric and
biolabo kit methods have been used for determination of iron (II),
iron (III) and total iron. The present methods have the advantages
of high sensitivity, low cost reagent, low operation cost,
simplicity, speed and their applications for determination of iron
(II) and iron (III) in some serum samples of normal human and
β-thallasemia patients in Erbil city.
For the first time especially in Erbil city attempts were made
to use zero, first and second derivative spectra to identify the
serum samples of some β-thallasemia patients from the normal
human serum samples due to the appearance and resolution of
peaks in both cases.
Keywords: Determination; Iron; ß-Thalassemia Patients;
Analytical Techniques;
4 th EucheMs chemistry congress
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deveLoPMent of new CoLoriMetriC redox
indiCAtorS for nAdh viSuALizAtion
M. foMin 1 , n. SteinKe 1 , B. KÖniG 2 , C. horn 1
1 Roche Diagnostics GmbH, Diabetes Care, Mannheim,
Germany
2 Universität Regensburg, Institut für Organische Chemie,
Regensburg, Germany
Nicotinamide adenine dinucleotide/reduced nicotinamide
adenine dinucleotide (NAD+/NADH) plays an important role as
cofactor in numerous biocatalized processes, including energy
metabolism, mitochondrial responses, immunological functions,
aging and cell death. Particularly, the NAD+/NADH couple acts
as the cofactor taking part in more than 300 dehydrogenase
enzymatic reactions. Intracellular NADH also can serve as a
natural biomarker for cellular redox reactions, a myriad of
metabolic activities, and mitochondrial anomalies under different
pathophysiological conditions. Consequently, numerous strategies
have been proposed for NADH detection, including fluorescence
imaging, enzymatic assay, high-performance liquid
chromatography, electrospray ionization mass spectrometry,
capillary electrophoresis, and electrochemical assay. [1] Under
certain conditions, a colorimetric methodology may be preferred.
For example, colorimetric assays are not sensitive to
autofluorescence background, do not require complex instruments
and demonstrate high sensitivities. [2] However existing redox
indicators have multiple disadvantages: limits in UV and green
range of light, poor solubility in water, low reactivity, lability to
UV-light, water and oxygen.
Quinones have been known as representative organic
electron acceptors for NADH oxidation due to their strong
oxidizing ability as well as stability of the reduced species. It was
found that UV-vis spectrum of quinone derivatives after NADH
reduction exhibit absorptions in the longer-wavelength region. [3]
With these in mind, we have decided to design new derivatives
and analogues of quinones and test them for NADH visualization.
The NADH reduction of new quinone-type electron
acceptors was seen by naked eye and monitored by UV-vis
extinction spectra. Design, synthesis and UV-vis properties of
several new indicators for NADH detection will be discussed.
references:
1. Liu, S.; Du, Z.; Li, P.; Li, F. Biosensors and Bioelectronics
2012, (in press).
2. Zhu, A.; Romero, R.; Petty, H. R. Analytical Biochemistry
2011, 419, 266.
3. Heindl, D.; Herrmann, D.; Hönes, D.; Josel, D.;
Junius-Comer, D.; Merdes, D.; Schmidt, D.;
Selbertinger, D.; U. S. Patent 6,057,120: May 2, 2000.
Keywords: Redox chemistry; Biosensors; Dyes/Pigments;
Cofactors; Quinones;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc