4th EucheMs chemistry congress

wednesday, 29-Aug 2012
s609
chem. Listy 106, s587–s1425 (2012)
Analytical chemistry Electrochemistry, Analysis, sample manipulation
Biosensor strategies
o - 2 6 1
BioSenSorS BASed on CArBon nAnotuBeS: the
roLe of CALiBrAtion on the reProduCiBiLity
of deviCeS
A. duArte 1 , C. JuStino 2 , J. P. AMArAL 3 ,
S. CArdoSo 3 , t. roChA-SAntoS 4
1 University of Aveiro, Department of Chemistry, Aveiro,
Portugal
2 University of Aveiro, Department of Chemistry and CESAM,
Aveiro, Portugal
3 INESC-MN, INESC-MN, Lisbon, Portugal
4 Instituto Piaget / University of Aveiro, ISEIT / Department of
Chemistry and CESAM, Viseu /Aveiro, Portugal
The analytical performance of biosensing technologies has
been enhanced by the incorporation of carbon nanotubes (CNT),
which display excellent electronic, chemical and structural
properties. Furthermore, the portability, functionality, and
reliability of CNT-based biosensors have been improved, thus
contributing to real-time, fast and accurate diagnosis. Recently,
biosensors based on networks of CNT with field effect transistors
(NTFET) have been developed to enhance the miniaturization of
devices and associated advantageous characteristics. However the
reproducibility of such biosensing devices has been affected when
analytical data of individual devices (with the same configuration
and characteristics) are compared. Such is due to the random
nature of CNT, i.e., variation on their diameter and chirality and
CNT density on networks. In this communication, some analytical
approaches such as the calibration of biosensing devices were
discussed as a way to solve the device-to-device variation; then,
the reproducibility of nanomaterials-based FET devices could be
improved for their practical application and commercialization.
Furthermore, an additional example based on the development of
NTFET devices for the detection of C-reactive protein is
demonstrated, where the variation on the analytical response was
checked, and a data analysis was performed to find analytical
similarities among individual devices.
Acknowledgements: FEDER under the Operational Program
for Competitiveness Factors – COMPETE, FCT (Fundacao
para a Ciencia e a Tecnologia, Portugal) within the framework
of research project CARDIOSENSOR (references FCOMP-01-
-0124-FEDER-010902 and PTDC/SAU-BEB/099042/2008).
This work was also funded through scholarships (references
SFRH/BD/60429/2009, and SFRH/BPD/65410/2009) under
QREN-POPH funds, co-financed by the European Social Fund
and Portuguese National Funds from MCTES.
Keywords: biosensors; nanotubes; immunochemistry;
Biosensor strategies
4 th EucheMs chemistry congress
o - 2 6 2
GLuCoSe oxidASe-funCtionALized
MeSoPorouS zirConiA thin fiLMS for
eLeCtroCheMiCAL GLuCoSe deteCtion
y. S. Ko 1 , S. y. Choi 2 , y. u. Kwon 1
1 Sungkyunkwan University, Chemistry, Suwon, Republic of
Korea
2 Sungkyunkwan University, SKKU Advanced Institute of
Nanotechnology, Suwon, Republic of Korea
We synthesized mesoporous zirconia thin films (MZTFs) by
using a mixed solution in which zirconium hydroxide
nanoparticles were self-assembled with a structure directing agent,
F127 (PEO PPO PEO , EO = ethylene oxide, PO = propylene
106 70 106
oxide). The mesostructures of these films were characterized by
low-angle X-ray diffraction, scanning electron microscopy,
transmission electron microscopy, and grazing incidence small
angle X-ray scattering. The 3D pore structures were identified by
two distinct ones with the Fmmm structure and the P6 /mmc 3
structure. The Fmmm structure has interconnected pores and the
P6 /mmc one less accessible pores. The MZTFs, formed on
3
fluorine-doped tin oxide electrodes, were functionalized with
glucose oxidase (GOx) and were studied for their potentials as
electrochemical sensor for glucose. The MZTF with the Fmmm
structure shows a large capacity for the adsorption of GOx. Thus,
the GOx-functionalized electrode of this MZTF showed high
sensitivity to glucose in a broad range of glucose concentration
of 1 - 7 mM. The electrochemical data of this electrode suggest a
surface-controlled mechanism, high degree of reproducibility, and
a long life time.
Keywords: Mesoporous film; Zirconia; Electrochemical
glucose detection;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc