4th EucheMs chemistry congress

Poster Session 1
s926
chem. Listy 106, s587–s1425 (2012)
Poster session 1 - Mining and the Environment
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ASturiAn MerCury MininG diStriCt(SPAin)And
the environMent: A review
J. Loredo 1 , A. ordonez 1 , r. ALvArez 1
1 University of Oviedo, Prospecting and Mining Exploration,
Oviedo, Spain
Mercury is of particular concern amongst global
environmental pollutants, with contaminated sites abundant
worldwide, many of which are associated with mining activities.
Northwest of Spain (Asturias) can be considered a Hg
metallogenic province with abundant deposits, whose paragenesis
includealso As-rich minerals. Several mines have operated in this
region since antiquity, with evidence of activity on individual sites
since the 1st and 2nd centuries. This leaded Asturias to become an
important Hg producer at the world scale during the decade
1962–1972, with average production of 15,000 flasks/year. Taking
into account the losses during mining and inefficient smelting, the
release of Hg and associated elements into the environment, (of
which As is of particular note), was significant.As occurred in
other Hg mining districts all around the world, the great decline
on Hg prices on the 1970’s gave rise to the closure of
Asturianmines. At that time, the environmental concern was not
the current one, and mines were abandoned long before the
introduction of any environmental regulations to control metal
release from associated spoil heaps and tailings ponds.
Aninheritanceof derelict underground and surface mine facilities
(shafts, galleries, and external buildings) and spoil heaps, still
remains in the sites of this district. Consequently, the environment
is affected globally, as high metal concentrations (mainly Hg and
As) have been found in soils, waters, sediments and air,and the
potential adverse health effects of the exposure to these elements,
have been assessed.
4 th EucheMs chemistry congress
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ChArACterizAtion of MiCroALGAe iSoLAted
froM ACid Mine drAinAGe (AMd) AS A
PotentiAL SourCe for BiodieSeL ProduCtion
y. PArK 1 , h. S. yun 1 , w. r. Lee 1 , J. Choi 1
1 KIST, Gangneung Institute, Gangneung, Republic of Korea
In an era of increasing oil prices and climate concerns,
biodiesel has gained attention as alternative energy sources.
Biodiesel derived from microalgae is a potential renewable and
carbon neutral alternative to petroleum fuels. One of the most
important decisions in obtaining oil from microalgae is the choice
of algal species. A total of 32 microalgae cultures were isolated
from an AMD (Acid Mine Drainage) in South Korea.
5 microalgae isolates were selected based on their morphology
and ease of cultivation under our test conditions. The name of
microalgae Nephroselmis sp. KGE 1, Nephroselmis sp. KGE 2,
Uronema sp. KGE 8, Scenedesmus obiquus KGE 9 and
Micractinium sp. KGE 10, based on 28S rRNA (D1D2 forward
primer 5'-AGCGGAGGAAAAGAAACTA-3',D1D2 revers
primer 5'- TACTAGAAGGTTCGAT TA GT C-3') sequence
analyses. Nephroselmis sp. KGE 2 maximum growth of biomass
concentration of 1.45±0.15 g dwt L-1 with a relatively lipid
content of 20±0.5% w/w. Also, the fatty acids compositions of the
studied species were mainly myristic, palmitic, palmitoleic, oleic,
linoleic, g-linolenic, and linolenic acids. High concentration of
oleic acid was also found in Nephroselmis sp. KGE 2, which led
us to conclude that fatty acids of microalgae incubated in growth
media were more effective for biodiesel production due to
relatively lower saturation degree compared to the other cultures
algal species. Our results suggest that Nephroselmis sp. KGE 2
can be a possible candidate species to produce oils for high quality
biodiesel.
Keywords: Microalgae; Biodiesel; Acid Mine Drainage; Oleic
acid; lipid;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc