4th EucheMs chemistry congress

Poster Session 1
s899
chem. Listy 106, s587–s1425 (2012)
Poster session 1 - life sciences
P - 0 0 7 8
quAntitAtive AnALySiS for the dnA StABiLity
towArd the deveLoPMent of new
funCtionAL MAteriALS
n. SuGiMoto 1 , h. yAKu 2 , t. MurAShiMA 1 ,
d. MiyoShi 1 , S. nAKAno 1 , t. endoh 3 ,
h. tAteiShi-KAriMAtA 3
1 Konan University, Frontier Institute for Biomolecular
Engineering Research and Faculty of Frontiers of Innovative
Research in Science and Technology, Kobe, Japan
2 Konan University and Panasonic Corporation, Frontier
Institute for Biomolecular Engineering Research and Faculty
of Frontiers of Innovative Research in Science and Technology
and 3Advanced Technology Research Laboratories, Kobe,
Japan
3 Konan University, Frontier Institute for Biomolecular
Engineering Research, Kobe, Japan
DNAs have been attracting attention as the advanced
materials such as biosensors, biodevices, and biocircuits due to
specific base-pairing interactions of DNAs. However, for DNA
materials to become practically applicable, finding a medium in
which they are stable for long time periods at room temperature
is an important bottleneck in this field. Room-temperature ionic
liquids (ILs) have generated tremendous interest as non-volatile
media that provide favourable environments for a wide
range of chemical and biochemical reactions. Choline
dihydrogenphosphate (choline dhp) with 20% dissolved water is
hydrated IL, which has been demonstrated to be a good solvent
for proteins and DNAs for ensuring long-term stability. It is
considered that the hydrared IL should be useful as a medium
developing DNA materials because the hydrated IL can keep the
activity of biomolecules for long time. However, its detail effect
on the DNAs remains unclear.
Here, we have investigated quantitatively thermodynamic
stabilities of DNA duplexes, triplexes and G-quadruplexes by
ultraviolet melting in the solution containing choline dhp. Choline
dhp induced a drastic change in the DNA stabilities depending on
their structures compared with an aqueous solution. In the
presentation, we will discuss the stability changes of DNA
structures from the perspective of thermodynamic parameters for
the DNA structure formations. Moreover, we will show our recent
works regarding quantitative analyses for the transcription,
translation and telomerase elongation reactions toward the
development of new functional materials.
Keywords: DNA; Thermodynamics; Ionic liquids;
4 th EucheMs chemistry congress
P - 0 0 7 9
SyntheSiS, AnALySiS And
AntioxidAntProPertieS of SChiff BASe
indoLe-AMino ACid derivAtiveS
S. Suzen 1 , S. S. CihAner 1 , t. CoBAn 2
1 Ankara University Faculty of Pharmacy, Pharmaceutical
Chemistry, Ankara, Turkey
2 Ankara University Faculty of Pharmacy, Pharmaceutical
Toxicology, Ankara, Turkey
Increased levels of reactive oxygen species attributed to
oxidative stress have been found to be responsible for the
development of some vital diseases such as cardiovascular,
neurodegenerative and autoimmune diseases. Recently, it was
observed that melatonin is a highly important antioxidant, and
melatonin analogues are under investigation to find out improved
antioxidant activity. In this study, 14 indole amino acid and
N-protected amino acid derivatives were synthesized and
elucidated spectrometrically. To investigate the antioxidant
activity of the synthesized compounds and to compare with
melatonin, butylhydroxytoluene and vitamin E, lipid peroxidation
inhibition and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging
activities were tested. The results indicated that the synthesized
new indole amino acid derivatives have similar activities to
melatonin in 2,2-diphenyl-1-picrylhydrazyl radical-scavenging
activity assay but more potent activities in lipid peroxidation
inhibition assay.
Keywords: indole; amino acid; synthesis; analysis;
antioxidant;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc