4th EucheMs chemistry congress

Poster Session 1
s911
chem. Listy 106, s587–s1425 (2012)
Poster session 1 - Environmental Chemistry
P - 0 1 0 1
the effeCtS of niCKeL on the MinerAL
nutrition of PoPLAr’S CLoneS And their
PhytoextrACtion PotentiAL
M. KeBert 1 , S. orLoviC 2 , B. KLASnJA 3 , d. StAJner 4 ,
B. PoPoviC 4
1 Institute of Lowland Forestry And Environment,
Environmental biochemistry, Novi Sad, Serbia
2 Institute of Lowland Forestry And Environment, General
forestry, Novi Sad, Serbia
3 Institute of Lowland Forestry And Environment, Wood
biotechnology, Novi Sad, Serbia
4 Faculty of Agriculture, Chemistry department, Novi Sad,
Serbia
Poplars present ideal wood model system for testing
phytoremediation of heavy metals contaminated soils as an
emerging and cost-effective technology due to fact that they act
as a biological filters and biomonitors. On the other hand,
contamination with Ni2+ ions detrimentally effect biochemical and
physiological processes as well as mineral nutrition in plants.
Especially, due to fact that Ni2+ as a trace metal has same charge
and similar atomic radii as some macronutrients such as Ca2+ and
Mg2+ , disturbances in their transport are expected.
In this study, experimental trial was settled in greenhouse,
in half-controlled conditions with three poplar clones cuttings
(B229, PE 19/66 and M1) which grown and were exposed to
different concentrations of Ni2+ (25, 50, 100 and 150 mg/kg). The
aim of this study was to asses differences in poplar’s clones
phytoextraction potential as well as changes in mineral nutrition
in poplar clones induced by nickel contaminated soil. Using
atomic absorption spectrophotometry (AAS), distribution of
absorbed Ni by organs (root, shoot and leaves) as well as contents
of Ca and Mg were determined in poplar clones. Also, N, C and
P contents were determined by elemental analysis (C,N) and
spectrophotometrically (P). Gained results showed that by
enhancement of applied Ni concentration in soil, accumulated Ni
increased in all examined plant organs in all clones, where clone
PE 19/66 showed highest phytoremediation potential among other
clones by accumulating 210,12 and 143,513 ppm in roots and
leaves, respectively at highest treatment. Obtained results should
be used in selection of nickel tolerant poplar clone with interesting
perspectives of application in phytoremediation technologies and
as a platform for forestation programs of Ni polluted soils.
Keywords: poplar; nickel; phytoextraction; mineral nutrition;
phytoremediation;
4 th EucheMs chemistry congress
P - 0 1 0 2
how to eStiMAte the PhytoextrACtion
PotentiAL of PLAntS?
A. KÖtSChAu 1 , G. BüCheL 1 , J. einAx 2 ,
w. von tüMPLinG 3 , d. Merten 1
1 Friedrich Schiller University Jena, Applied Geology, Jena,
Germany
2 Friedrich Schiller University Jena, Environmental Analysis,
Jena, Germany
3 Hemholtz Centre for Environemental Research, River Ecology,
Magdeburg, Germany
Phytoremediation describes the cleaning of polluted
environments by use of plants. A special way to remediate is to
extract heavy metals from soil through uptake by plants.
Advantages of phytoremediation are the low costs and a high
acceptance in the population. A promising phytoextraction plant
is the sunflower (Helianthus annuus).To test its potential under
real conditions we grew sunflowers on the testfield
“Gessenwiese”, which is situated in the former uranium mining
district of Ronneburg (Thuringia). This area is contaminated with
a broad range of heavy metals such as Cd, Cr, Co, Cu, Ni, Th, U
and Zn and also shows elevated values for rare earth elements
(REE). The harvested sunflowers were analyzed for the above
named elements in roots and above-ground plant parts. The soil
was analyzed for the total content, the bioavailable fraction and
the specifically adsorbed fraction (Zeien and Brümmer, 1989).
Often the phytoextraction potential of a plant is estimated based
on first: transfer factor (TF) - content ratio of roots vs. soil,
second: translocation factor (TL) - content ratio of above-ground
plant part vs. roots, and third: bioaccumulation factor (BAF)
– content ratio of above-ground plant parts vs. soil. Generally, for
soil the total elemental content is taken into account. To our
opinion this is not satisfying, since the content determined by total
digestions contain a high fraction which is not available to the
plants. Because of this drawback we additionally calculated TF
and BAF with the bioavailable and specifically adsorbed soil
fractions. This revealed much higher TF and BAF, compared to
total soil calculation, so that the sunflower can be claimed as a
good metal extractor. In addition it shows, that the plants
solubilize even more metals, than easy available for them, which
means a high energy effort for them.
Keywords: Phytoextraction; Transferfactor;
Bioaccumulationfactor; Sunflower; Heavy metals;
AUGUst 26–30, 2012, PrAGUE, cZEcH rEPUbLIc