Electrophoresis

Electrophoresis · Agarose and Gelling
0.5 % agarose in 1 x TAE
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Agarose Broad Range
DNAse-free
RNAse-free
Roti ® garose for gel electrophoresis, total size range
• High-molcular linear polymer of particularly high DNA mobility
in electrophoresis
• The ideal choice for Pulse Field gel electrophoresis
• Excellent for resolving DNAfragments between 200 bp and 40 kb
in conventional gel electrophoresis
• Low EEO allows speedy and efficient resolution of very large
DNA-molecules
• Extremely high gel strength guarantees good handling even at
low concentrations of 0.3 % agarose
• Also suitable for separation of particles like ribosomes,
virions or chromosomes
Guarantee analysis:
Electroendosmosis (EEO). ..................................................................................≤0,12
Ash content......................................................................................................≤0,25 %
Sulphate (SO4 ). ...............................................................................................≤0,12 %
Turbidity (1.5 % solution). ................................................................................ ≤4 NTU
Loss on drying. .....................................................................................................≤7 %
Gel strength (1 % gel)...........................................................................≥1800 g/cm2 Gel strength (1.5 % gel)........................................................................≥3200 g/cm2 Gelling temperature. .................................................................................34,5-37,5 °C
Melting temperature..................................................................................86,5-89,5 °C
DNA-separation (≥1000 bp)............................................................................complies
DNases, RNases. .................................................................................. none detected
T846.1 10 g plastic 19,40 €
T846.2 100 g plastic 103,60 €
T846.3 500 g plastic 440,75 €
Original pack 6 x 500 g 418,71/500 g
• Ethidium bromide solutions -
as high concentrated stock
solution or ready-to-use in
convenient dropper bottles
see page 874
0.3 resp. 0.5 % agarose in 1 x TAE
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Agarose Pulsed-Field
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
Roti ® garose for Pulsed Field electrophoresis,
for genetic engineering
Special agarose for separation of large fragments.
• Optimised for separation of fragments of 20 to 50 kb or for
Pulsed Field Gel Electrophoresis (PFGE)
• High gel-strength agarose for tearproof gels that are easy to handle
• High DNA mobility for fast and efficient gel electrophoresis
• Low background fluorescence after ethidium bromide staining
• In general, suitable for separation of fragments larger than 1000 bp
• Tested for absence of DNAses, RNAses and DNA binding
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DNAse-free
RNAse-free
Figure (left to right):
0.3 % Agarose Pulsed-Field, extended 1kbp Ladder (1 µg) and
High Molecular Weight Marker (0.3 µg, heated for 10 mins. at 65 °C);
then same markers on 0.5 % Agarose Pulsed-Field.
Guarantee analysis:
Electroendosmosis (EEO)................................................................................≤0,06
Sulphate (SO4). ...............................................................................................≤0,12 %
Loss on drying. ...................................................................................................≤10 %
Gel strength (1 % gel)...........................................................................≥1700 g/cm2 Gelling temperature. .......................................................................................36 ±2 °C
Melting temperature........................................................................................90-95 °C
DNases, RNases. .................................................................................. none detected
3771.1 10 g plastic 23,85 €
3771.2 25 g plastic 43,25 €
3771.3 100 g plastic 135,10 €
3771.4 250 g plastic 280,90 €
Agarose as Gelling Reagent
Chemically, agarose is a galactan which is capable of forming extremely solid gels,
even at a low concentration. The pore size of the gels is determined by the
concentration of the agarose used. Because there are no ionic groups in the gel,
hydrophilic materials without any interaction with the gel matrix will also be separated
according to their size. The high hysteresis of the agarose, that is the thermal stability
after solidification, ensures that the gels remain reliably stable even during
heat-producing running conditions.
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Elektrophorese