Electrophoresis

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<strong>Electrophoresis</strong> · PAGE Precast Gels Roti ® -PAGE Precast Gels ready-to-use, for rapid and easy protein gel electrophoresis • Sharp resolution • Very easy application • Compatible with most minigels chambers • Shelf life: 12 weeks from date of manufacture • Well tested Rotiphorese ® quality • Suitable for denaturing and native electrophoresis • Consistent lot-to-lot and get-to-gel performance • In-process controlled manufacturing and functional testing of every lot • Well suited and optimised for all standard down-stream applications, like, e.g. gel staining (Coomassie, fluorescence, silver), gel drying (vacuum, gel drying frames) and Western-Blotting (Semi-Dry, Tank) re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use Gel parameters: Cassette size (LxB): 9 x 10 and 10 x 10 cm Gel size (LxB): 7.1 x 8.3 bzw. 8.1 x 8.3 cm Gel thickness: 1 mm Gel matrix continuous (10 % or 12 % polyacrylamide) or in gradient (4 - 20 %) Stacking gel 4 % polyacrylamide Buffer system Tris/Glycine without SDS Package contains Tris/Glycin buffer with 0.02 % sodium azide Precast mini gels for rapid and easy gel electrophoresis in laboratory and students courses. Roti ® -PAGE mini gels come precast and are very easy and quick to use. Roti ® -PAGE with 12 % gel matrix or gradient gel matrix (4-20 %) Coomassie Staining. Denaturing gel run in Tris/Glycin buffer with 1 % SDS. 1 hour, 25 mA (150 V, ramping to 300 V). Lane 1-2: 8 µl Roti ® -Mark STANDARD (Art. No. T851); Lane 3-4: 5 µl Roti ® -Mark PRESTAINED (Art. No. T852); Lane 5-6: 5 µl Roti ® -Mark BICOLOR (Art. No. 2806); Lane 7-8: 7,5 µl Roti ® -Mark 10-150 (Art. No. T850); Lane 9-10: 7,5 µl Roti ® -Mark WESTERN (Art. No. 0948). Our proven and tested Rotiphorese ® quality and the standardised tris-glycine buffer system are compatible with all running and loading buffers according to Lämmli and all downstream applications (Western Blot, MS-analysis, sequencing etc.). All Roti ® -PAGE Minigels include 4 % polyacrylamide stacking gels and yield a sharp band separation in the recommended protein range. Because of their variable format of 9 x 10 cm or 10 x 10 cm, the gel plates fit all standard mini-electrophoresis chambers and can be separated easily after the run without damaging the precious gel. The gel matrix contains no SDS (for native gels), a running buffer containing SDS is recommended for denaturing gels (e.g. Rotiphorese ® 10x SDS-PAGE, Art. No. 3060). Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Store at +4 °C Transport temp.: cooled Gel plates may be separated very easily by using the comb without tearing the precious gel. 847 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · PAGE Precast Gels Compatibility Both gel sizes of Roti ® -PAGE Precast Gels (9 x 10 cm and 10 x 10 cm) are well compatible with both series of Roth's vertical electrophoresis units, classical green line (Art. No. Y001-Y004) and injection-moulded line Rotiphorese ® -PROclamp (Art. No. 3501-3503). Unit 9 x 10 cm 10 x 10 cm BioRad ® Mini PROTEAN ® I, II & III and Ready Gel ® Cell yes* not comp. CBS Scientific MGV System 10 x 8 cm unit yes not comp. CBS Scientific MGV System 11.3 x 10 cm unit not comp. yes Cleaver Scientific omniPAGE mini yes yes Fisher EC 120-2 yes yes Fisher FBVE units not comp. yes* Gibco BRL Mini-V8 yes not comp. Hoefer Mighty Small SE 250*, 260 & 280 yes yes Hoefer Mini VE not comp. yes Invitrogen Novex ® Xcell Units (incl. MiniCell) yes yes Life Technologies - BioMetra ® Mini V yes not comp. LONZA PAGEr ® Minigel Chamber yes yes Novex ® Xcell Units (incl. MiniCell) yes yes peQLab Perfect Blue TM Twin S yes yes Scieplas TV50 and TV100 yes yes Serva BlueVertical TM Mini Slab Gel System BV 102 yes yes Sigma Aldrich Mini Techware 10 x 8 cm unit yes not comp. Sigma Aldrich Mini Techware 11.3 x 10 cm unit not comp. yes Thermo Scientific EC 120 mini vertical gel system yes yes Thermo Scientific Owl Penguin TM Modell P8DS-1 not comp. yes * with slight modification of the device Summary PAGE Precast Gels Roti ® -PAGE 10 % continuous matrix for proteins of 25 to 200 kD Roti ® -PAGE 12 % continuous matrix for proteins of 20 to 100 kD Roti ® -PAGE Gradient (4-20 %) Matrix in Gradient 4-20 % for proteins of 5 to 200 kD Roti ® -PAGE Gradient (4-20 %) Matrix in Gradient 4-20 % for proteins of 5 to 200 kD Important information on refrigerated transports 848 All products identified as being particularly temperature-sensitive are shipped in special ice boxes with freezer packs or in dry ice. Additional costs resulting from such, will be invoiced (further informations on request). Please note: In order to guarantee optimum product quality, the dispatch of refrigerated transport products will be carried out on Mon and Tue only outside Germany! Slight delays in delivery are therefore possible. For units of other suppliers the adjacent list applies. In case other units are used or special adaptors are required, please ask our technical service under info@carlroth.com Store at +4 °C Transport temp.: cooled Wells Gel plates Art. No. Pack Qty. € 10 9x10 cm 2838.1 2838.2 2 10 31,90 135,10 10 10x10 cm 2839.1 2839.2 2 10 31,90 135,10 10 9x10 cm 2840.1 2840.2 2 10 31,90 135,10 10 10x10 cm 2841.1 2841.2 2 10 31,90 135,10 10 9x10 cm 2842.1 2842.2 2 10 31,90 135,10 10 10x10 cm 2843.1 2843.2 2 10 31,90 135,10 12 9x10 cm 2844.1 2844.2 2 10 31,90 135,10 12 10x10 cm 2845.1 2845.2 2 10 31,90 135,10 Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593
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Electrophoresis

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