Electrophoresis

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Electrophoresis · Gel Staining Rotiphorese ® Blue R re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use Brillant Blue R concentrate For Coomassie staining of protein gels. l n i Danger H225-H370-H315-H319-H301-H311-H331 Concentrate for easy preparation of Coomassie Blue R staining solutions. Rotiphorese ® -blue is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture. Figure: Gel stained by Rotiphorese ® -Blue R, 12 % PAA in Tris, Roti ® -Mark STANDARD (Art. No. T851). Concentrate contains Coomassie Blue R, methanol and pure acetic acid. Rotiphorese ® concentrate Blue R can be diluted with 400-600 ml deionised water to obtain the following compositions: Dilution with water 397.5 ml 507.5 ml 602.5 ml yields staining solution approx. 1 l approx. 1.1 l approx. 1.2 l methanol 50 % 45 % 41.5 % pure acetic acid 10 % 9 % 8.3 % water 39.75 % 45.78 % 50 % Blue R 0.25 % 0.22 % 0.2 % Dilution can be carried out in the supplied bottle. The solution should be filtered before use. 3074.1 600 ml glass 25,65 € Brilliant Blue R 250 (C.I. 42660) for preparing staining solution for the protein analysis in gel electrophoresis Coomassie Brillant Blue R 250 C 45 H 44 N 3 NaO 7 S 2 M 825,99 g/mol Only conditionally use for staining of membranes after protein transfer. 3862.1 10 g glass 18,10 € 3862.2 100 g glass 71,80 € • White light-light boxes see page 559 Safety data sheets can be found on the Internet next to the product description. Roti ® -Black P silver staining kit for proteins o h i n j Danger H272-H301-H311-H331-H314-H317-H351-H410 • Sensitive silver staining • Transparent gel • Reliable results Roti ® -Black is a kit for speedy, reproducible silver staining of proteins in free and foil-supported gels. High sensitivity in combination with very low background enables detection of proteins from 1 ng/mm 2 on. 1 kit is adequate for staining 10 gels. Staining can be performed in unsupported gels without methanol. Included is an easy-to-follow instruction manual which will guarantee reliable results. L533.1 for 10 gels of 10 ml vol. each 59,80 € L533.2 for 10 gels of 40 ml vol. each 75,90 € Silver staining of proteins and DNA-gels Roti ® -Black silver staining kits have been optimized to provide you with the most sensitive staining possible. Adding thiosulfate during the sensibilization and generation process reduces the background and increases contrast (acc. to Blum et al., (1987) Electrophoresis 8:93-9). Formaldehyde should preferably be fresh when applied and it reduces the silver ions (Ag + ) to so-called silver seed crystals. We recommend to: • Wash the gels carefully after each step otherwise there may be side reactions in the form of stains, spots, increased background or secondary bands. • Fix the gels carefully. Inadequate fixation impairs band formation and increases the background. Fresh and high-quality fixatives should be used and adapted to the volume and duration of gel strength. Please note our Technical Information Brochure on the Internet (Life Science Main Page): Coomassie Gel Staining Basics and mechanisms of Coomassie dyes Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 871 Elektrophorese
Elektrophorese Electrophoresis · Gel Staining Roti ® -Bleach decolorisation kit for silver staining Store at +20 °C Easy decolorising of overstained silver gels in foil-supported or free PAA gels. The new staining consistency is firmly preserved. Fig.: A) Overstained silver gel B) Same gel after subsequent optimisation with Roti ® -Bleach The kit is suitable for both DNA and proteins. One kit is sufficient for 10 gels à 10 ml or 5 gels à 40 ml. N766.1 kit carton 51,15 € Silver nitrate ≥99.9 %, for staining of proteins AgNO 3 M 169,88 g/mol o h j Danger H272-H314-H400-H410 Proteicolor is a special quality silver nitrate for reproducible staining of proteins and 1,2 Diol-groups, of glycoproteins and polysaccharides in polyacrylamide and agarose (7) gels. Staining with proteicolor can also be used for highly sensitive identification of RNA and DNA. 6207.1 50 g plastic 97,35 € Instructions for use can be found on the Internet next to the product description. 872 Roti ® -White Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 zinc staining kit for protein gels Store at +20 °C h g n j Danger H302-H314-H361-H400-H410 Roti ® -White is a kit for reverse staining of proteins in gels. Proteins which can be stained with Coomassie only with difficulty are frequently stainable with Roti ® -White. Background turns white and the areas in the gel, where proteins can be located, remain transparent. Membrane transfer as well as protein elution can be performed subsequently without reservation. Since diffusion of proteins is restricted in the gel after staining with Roti ® -White, analyses may be interrupted prior to the transfer elution. Detection limit is 15 ng/mm 2 . The kit is sufficient for 50 gels with up to 24 ml. Please note: Roti ® -White is not applicable to all ready-made gels. We recommend using Rotiphorese ® ready-to-use gel solutions. K063.1 1 kit 74,00 € Gel Staining Trays Compatible with all standard staining methods Wiederverwendbar Reusable The staining trays, available in two sizes, are a practical and cheap alternative to plastic household bowls. The trays are compatible with all standard staining methods and thanks to the tightly-sealing, recessed lids, can be stacked safely and without slipping on the shaker. The asymmetric array of the marking on the edges and drainage outlets on all corners guarantee easy handling and simple orientation of gel. ➀ The PP (polypropylene) trays are heat-resistant, microwave-proof and excellent for staining protein/peptide gels. Type Material Dimensions L x W x H (mm) Art. No. Pack Qty. € MINI PP 113 x 75 x 25 CP57.1 20 79,50 MIDI PP 143 x 100 x 25 CP58.1 20 106,20
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Electrophoresis

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