Electrophoresis

More documents

Recommendations

Info

<strong>Electrophoresis</strong> · Gel Staining Staining of DNA Gels DNA colour reagents for agarose and acrylamide gels, for laboratories and practical applications. We offer you high-quality stains with high sensitivity (silver stains), standard stains (ethidium bromide) in userfriendly dropper bottles, and easy-to-use stains for school experiments. The following table provides an overview of the various stains and their respective sensitivity levels. Staining Sensitivity Description Roti ® -Black N
Elektrophorese <strong>Electrophoresis</strong> · Gel Staining Fluorescent Staining of Nucleic Acids Ethidium bromide-free staining New, non-toxic and non-carcinogenic staining dye for fluorescent staining of nucleic acids in agarose gels. Highly sensitive and applied just like ethidium bromide. Resulting brightly-green signals may be detected by standard ethidium bromide photo filters. Roti ® -Safe GelStain ready-to-use, for electrophoresis For fluorescent staining of nucleic acids in agarose gels. Store at +4 °C Roti ® -Safe GelStain is a new, versatile staining reagent for detection of all nucleic acids in agarose gels. Roti ® -Safe GelStain detects up to 1.5 ng/mm 2 nucleic acid, which is approx. 0.2 ng per band, and is, therefore, as sensitive as ethidium bromide. The dye may be used exactly in the same way as ethidium bromide - for staining in-gel, staining through running buffer, or post-run staining -, and may replace this toxic and potentially mutagen reagent completely. When bound to nucleic acids in agarose gels, Roti ® -Safe GelStain emits a brightly green fluorescence that may be documented by standard ethidium bromide foto filters. Roti ® -Safe GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection. Excitation maximum (bound to DNA): 290-320 nm Emission maximum (bound to DNA): 515 nm Suitable for staining of dsDNA, ssDNA and RNA. 1 ml is sufficient for staining of approx. 600 minigels (with 30 ml agarose each). 3865.1 1 ml plastic 64,85 € 3865.2 5 x 1 ml plastic 297,15 € Please note our Technical Information Brochures on the Internet (Life Science Main Page): Gel Staining of Agarose Gels Ethidiumbromide, methylene blue and Roti ® -Safe GelStain Roti ® -Safe GelStain Basics and Safety data 874 re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Ethidium bromide solutions In spite of newly developed fluorescent staining reagents ethidium bromide remains the standard staining technic for nucleic acid in electrophoresis. Ethidium bromide is a carcinogenic substance which shouldn't come in contact with skin. In order to minimise this danger we offer ready-to-use ethidium bromide solution in dropper bottles for easy handling and maximum safety. Excitation maxima (unbound): 210 nm, 285 nm Excitation maximum (bound to DNA): 366 nm Excitation possible via DNA at 254 nm Emission maximum: 605 nm. Ethidium bromide solution 0.025 % in dropper bottle aqueous solution (250 µg/ml) For staining of DNA or RNA in agarose gels during electrophoresis. i Danger H331 Application: Two drops will suffice for staining a 50 ml agarose gel. Add directly to melted agarose. Please store in the dark! HP47.1 15 ml plastic 24,70 € Ethidium bromide solution 0.5 % in dropper bottle aqueous solution (5 mg/ml) For counterstaining of nuclei in histology and cytology. For staining of DNA/RNA after electrophoresis. g Warning H332 Application: In order to prepare a staining solution add 5 drops of ethidium bromide solution to 500 ml water, buffer, or electrophoresis running buffer, respectively. Nuclei / nucleic acids are stained red. Please store in the dark! HP46.1 15 ml plastic 24,70 € Safety data sheets can be found on the Internet next to the product description. re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use
Page 1 and 2: SC Global Step SRL Electrophoresis
Page 3 and 4: Elektrophorese Electrophoresis · P
Page 7 and 8: Elektrophorese Electrophoresis · A
Page 15 and 16: Elektrophorese Electrophoresis · G
Page 17 and 18: Elektrophorese Electrophoresis · M
Page 25 and 26: Elektrophorese Electrophoresis · G
Page 27: Elektrophorese Electrophoresis · G
Page 31 and 32: Elektrophorese Electrophoresis · U
Page 51 and 52: Elektrophorese Electrophoresis · B

Electrophoresis

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?