Electrophoresis

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Electrophoresis · Agarose and Gelling 0.5 % agarose in 1 x TAE kb 14,7 - 9,8 - 4,9 - kb - 10 - 6 - 5 - 4 - 3 - 2 - 1 Agarose Broad Range DNAse-free RNAse-free Roti ® garose for gel electrophoresis, total size range • High-molcular linear polymer of particularly high DNA mobility in electrophoresis • The ideal choice for Pulse Field gel electrophoresis • Excellent for resolving DNAfragments between 200 bp and 40 kb in conventional gel electrophoresis • Low EEO allows speedy and efficient resolution of very large DNA-molecules • Extremely high gel strength guarantees good handling even at low concentrations of 0.3 % agarose • Also suitable for separation of particles like ribosomes, virions or chromosomes Guarantee analysis: Electroendosmosis (EEO). ..................................................................................≤0,12 Ash content......................................................................................................≤0,25 % Sulphate (SO4 ). ...............................................................................................≤0,12 % Turbidity (1.5 % solution). ................................................................................ ≤4 NTU Loss on drying. .....................................................................................................≤7 % Gel strength (1 % gel)...........................................................................≥1800 g/cm2 Gel strength (1.5 % gel)........................................................................≥3200 g/cm2 Gelling temperature. .................................................................................34,5-37,5 °C Melting temperature..................................................................................86,5-89,5 °C DNA-separation (≥1000 bp)............................................................................complies DNases, RNases. .................................................................................. none detected T846.1 10 g plastic 19,40 € T846.2 100 g plastic 103,60 € T846.3 500 g plastic 440,75 € Original pack 6 x 500 g 418,71/500 g • Ethidium bromide solutions - as high concentrated stock solution or ready-to-use in convenient dropper bottles see page 874 0.3 resp. 0.5 % agarose in 1 x TAE kb kb - 48 48 - - 19 - 12 19 - - 8 12 - 8 - - 4 4 - 1 - Agarose Pulsed-Field Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Roti ® garose for Pulsed Field electrophoresis, for genetic engineering Special agarose for separation of large fragments. • Optimised for separation of fragments of 20 to 50 kb or for Pulsed Field Gel Electrophoresis (PFGE) • High gel-strength agarose for tearproof gels that are easy to handle • High DNA mobility for fast and efficient gel electrophoresis • Low background fluorescence after ethidium bromide staining • In general, suitable for separation of fragments larger than 1000 bp • Tested for absence of DNAses, RNAses and DNA binding - 1 DNAse-free RNAse-free Figure (left to right): 0.3 % Agarose Pulsed-Field, extended 1kbp Ladder (1 µg) and High Molecular Weight Marker (0.3 µg, heated for 10 mins. at 65 °C); then same markers on 0.5 % Agarose Pulsed-Field. Guarantee analysis: Electroendosmosis (EEO)................................................................................≤0,06 Sulphate (SO4). ...............................................................................................≤0,12 % Loss on drying. ...................................................................................................≤10 % Gel strength (1 % gel)...........................................................................≥1700 g/cm2 Gelling temperature. .......................................................................................36 ±2 °C Melting temperature........................................................................................90-95 °C DNases, RNases. .................................................................................. none detected 3771.1 10 g plastic 23,85 € 3771.2 25 g plastic 43,25 € 3771.3 100 g plastic 135,10 € 3771.4 250 g plastic 280,90 € Agarose as Gelling Reagent Chemically, agarose is a galactan which is capable of forming extremely solid gels, even at a low concentration. The pore size of the gels is determined by the concentration of the agarose used. Because there are no ionic groups in the gel, hydrophilic materials without any interaction with the gel matrix will also be separated according to their size. The high hysteresis of the agarose, that is the thermal stability after solidification, ensures that the gels remain reliably stable even during heat-producing running conditions. 855 Elektrophorese
Elektrophorese Electrophoresis · Agarose and Gelling Agarose HR-PLUS Roti ® garose optimal fragment separation of 100 to 3000 bp Standard agarose for optimal fragment separation in the main analytical range. • Reliable agarose for routine analyses in the range of 100 to 3000 bp • Superior separation efficiency and high gel strength, usable in concentrations of up to 3 % agarose • Very well suited for analysis of PCR amplificates, restriction digests and for blotting • Low gel background • Compatible with all standard nucleic acid-staining methods • Separation range in 1 x TAE: 1 % proof agarose gel: 1000-3000 bp 1.5 % proof agarose gel: 300-2000 bp 2.5 % proof agarose gel: 100-1000 bp Guarantee analysis: Electroendosmosis (EEO). ..................................................................................≤0,12 Water content. ......................................................................................................≤7 % Ash. ......................................................................................................................≤4 % Sulphate (SO4). ...............................................................................................≤0,11 % Gel strength (1.5 % gel). ...........................................................................≥1500 g/cm2 Gelling temperature (1.5 % gel). ........................................................................~36 °C Melting temperature (1.5 % gel)........................................................................~ 88 °C Turbidity (1.5 % solution). ................................................................................ ≤4 NTU DNases, RNases. .................................................................................. none detected DNA binding................................................................................................. very low HP30.1 10 g plastic 39,55 € HP30.2 100 g plastic 245,10 € HP30.3 500 g plastic 1029,85 € Original pack 6 x 500 g 978,36/500 g Please note our Technical Information Brochure on the Internet (Life Science Main Page): Dye Migration in Agaroses Running distances of xylene cyanol and bromophenol blue 856 bp 2176 - 1030 - 517 - 298 - 154 - 1.5 % agarose in 1 x TAE bp - 2500 - 1500 - 1000 - 500 - 100 DNAse-free RNAse-free 3 % agarose in 1 x TAE bp 500 - 400 - 300 - 200 - 120 - Agarose High Resolution Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 bp - 1000 - 700 - 600 - 500 - 400 - 300 - 200 - 100 DNAse-free RNAse-free Roti ® garose high resolution
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Electrophoresis

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