Electrophoresis

Electrophoresis · Gel Staining
Ethidium bromide solution 1 %
1 % solution in water (10 mg/ml)
Homidium bromide, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium
bromide
i n Danger H330-H341
Stock solution for fluorescent detection of nucleic acids.
Detection limit: 1.5 ng/mm 2 .
Please store in the dark!
2218.1 10 ml glass 8,55 €
2218.4 15 ml dropper bottle 24,70 €
2218.2 30 ml glass 18,25 €
2218.3 100 ml glass 44,70 €
Ethidium bromide
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new
size
≥98 %
For DNA-staining.
Homidium bromide, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium
bromide
C 21 H 20 BrN 3
M 394,33 g/mol
Store at +20 °C
i n Danger H330-H341-H302
Fluorescent dye with specific DNA binding capacity. Intercalates between
base pair stacks of dsDNA, causing weaker staining of ssDNA or RNA.
For staining of DNA/RNA in gels and for counterstaining of nuclei in
histological and cytological analyses. Store in the dark!
Excitation maximum: 518 nm
Emission maximum: 605 nm
7870.1 1 g glass 18,30 €
7870.2 5 g glass 72,10 €
Ethidium bromide is a strong mutagenic and toxic substance. For your own safety,
always wear gloves and respiratory protection to prevent contamination when working
with this substance. After use, dispose of solutions correctly.
They may not be discarded into the environment.
The following procedure is recommended (acc. to INSERM, Dossier prevention,
Number 2, 2nd Ed., September 88):
1. Collect and dispose of as hazardous waste.
2. Break down EtBr with potassium permanganate and HCI
(P. Quillardet, M. Hofnung, Trends Genet. 4,89 [1988]).
3. Break down EtBr with hypophosphoric acid and sodium nitrite
(Lunn and Sansone; Analyt. Biochem. 162, 453-458 [1987]).
4. Absorption of EtBr with Amberlite ® XAD-16 (Lunn and Sansone) or with activated
carbon (Bensaude, O. Method, Trends Genet. 4, 89-90 [1988]) from the solution.
We recomment disposure by
Sekuroka ® Decon-Bags (Art. No. T856.1/.2/.3) or
Ethidium bromide extractors (Art. No. T857.1/2).
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
Disposure of ethidium bromide
Sekuroka ® Decon-bags
Absorber bags for removing ethidium bromide from liquids.
Sekuroka ® DeconBags are a simple method for removal of ethidium
bromide from solutions. The bags contain a special type of activated carbon
which absorbs ethidium bromide effectively and safely.
One bag can absorb 2.5 mg ethidium bromide.
Efficiency is >99 %. One pack contains 50 bags.
The application is extremely simple: Place the absorber bag in the ethidium
bromide solution, incubate for 24 hours under stirring and dispose of the
bag as hazardous waste in accordance with local regulations.
Also suitable for absorption of PicoGreen and SYBR Green!
Art. No. Pack Qty. €
T856.2 10 Bags 22,40
T856.1 50 Bags 87,75
T856.3 250 Bags 374,10
Ethidium Bromide Extractor
System for fast removal of ethidium bromide from gel staining
solutions.
Ethidium bromide is removed with an efficiency of >99 %.
Absorption capacity is up to 10 litres (at 0.5 mg/l ethidium bromide).
The extractor is suitable for bottle neck diameter of 33 to 45 mm.
The vessel is resistant to organic solvents.
Also suitable for absorption of PicoGreen and SYBR Green!
Total absorption capacity Art. No. Pack Qty. €
10 mg T857.1 Pack of 2 41,00
30 mg T857.2 Pack of 6 164,00
• Gel documentation system, Transilluminators,
UV-hand-held lamps and UV-hand-held
magnifiers see page 560
875
Elektrophorese