Electrophoresis

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<strong>Electrophoresis</strong> · Gel Loading Buffers Roti ® -Load DNA 1x (with glycerol) ready-to-use, DNase-free 1x gel loading buffer for DNA gel electrophoresis, excellent for resolubilisation of DNA pellets. Store at -20 °C Transport temp.: room temp. Each batch is tested for its funcionality in electrophoresis and proved to be DNase-free. The 1x loading buffer contains: Tris, sodium-acetat, EDTA, bromophenol blue, xylene cyanol and glycerol (see fig. 1). 0100.1 5 x 1 ml 24,75 € Roti ® -Load DNA short-run (with glycerol) DNase-free 6x gel loading buffer for high-throughput DNA-electrophoresis and short separation distance. Store at -20 °C Transport temp.: room temp. A special gel loading buffer without xylene cyanol and with slightly reduced bromophenol blue, optimised for high-throughput gel electrophoresis (see fig. 2). Additionally, the buffer may be diluted to 0.6fold if the bromophenol blue might cloud some DNA-bands. Each batch is tested for its functionality in electrophoresis. The 6x loading buffer contains: Tris, sodium-acetat, EDTA, bromophenol blue and glycerol. 0095.1 5 x 1,8 ml 32,25 € Roti ® -Load DNA short-run 1x (with glycerol) ready-to-use, DNase-free 1x gel loading buffer for high-throughput DNA-electrophoresis and short separation distance. Store at -20 °C Transport temp.: room temp. A special gel loading buffer without xylene cyanol and with slightly reduced bromophenol blue, optimised for high-throughput DNA-gel electrophoresis (see fig. 2). Additionally, the buffer may be diluted to 0.6fold if the bromophenol blue might cloud some DNA-bands. Each batch is tested for its functionality in electrophoresis. The 1x loading buffer contains: Tris, sodium-acetat, EDTA, bromophenol blue and glycerol. 0099.1 5 x 1 ml 23,45 € Roti ® -Load DNA small (with glycerol) DNase-free 6x gel loading buffer for DNA gel electrophoresis of small fragments. Store at -20 °C Transport temp.: room temp. A special gel loading buffer without bromophenol blue, optimised for gel electrophoresis of small fragments. Prevents DNA bands of small fragments from being concealed by bromophenol blue, even in high concentrated agarose gels (see fig. 3). Each batch is tested for its functionality in electrophoresis. The 6x loading buffer contains: Tris, sodium-acetat, EDTA, xylene cyanol and glycerol. HP03.1 5 x 1,8 ml 35,55 € Roti ® -Load DNA orange 1 (with glycerol) DNase-free 6x gel loading buffer for DNA gel electrophoresis, optimised for very short separation distances. Store at -20 °C Transport temp.: room temp. A special gel loading buffer with orange G as single dye, optimised for very short gel runs. Orange G migrates in parallel with very small DNA fragments, indicating approx. the maximal separation distance in very short runs (see fig. 6). Also very well suited in order to avoid concealing of important DNA-bands by bromophenol blue or xylene cyanol. Each batch is tested for its functionality in electrophoresis. The 6x loading buffer contains: Tris, sodium-acetat, EDTA, orange G and glycerol. HP04.1 5 x 1,8 ml 35,10 € Roti ® -Load DNA-orange 2 (with glycerol) DNase-free 6x gel loading buffer for DNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. Gel loading buffer with orange G and xylene cyanol (see fig. 5). Convenient for separation of a broad range of DNA-fragments in the gel. Also well suited in order to avoid concealing of important DNA-bands by bromophenol blue. Each batch is tested for its functionality in electrophoresis. The 6x loading buffer contains: Tris, sodium-acetat, EDTA, xylene cyanol, orange G and glycerol. HP05.1 5 x 1,8 ml 35,10 € Roti ® -Load DNA-tricolor (with glycerol) DNase-free 6x gel loading buffer for DNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. Gel loading buffer with orange G, bromophenol blue and xylene cyanol (see fig. 4). Convenient for separation of a broad range of DNA-fragments in the gel. Each batch is tested for its functionality in electrophoresis. The 6x loading buffer contains: Tris, sodium-acetat, EDTA, xylene cyanol, bromophenol blue, orange G and glycerol. HP06.1 5 x 1,8 ml 35,10 € Roti ® -Load RNA RNase-free 1.3x gel loading buffer for RNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. g n Danger H312-H360-H351-H302-H315-H319-H317-H335+H332 Roti ® -Load RNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in Roti ® -Load RNA. Each batch is tested for its functionality in electrophoresis and proved to be RNase-free. Roti ® -Load RNA contains: formamide, MOPS, Na-acetate, EDTA, formaldehyde, bromophenol blue and ethidium bromide; pH 7.0. Store in the dark. T848.1 2 x 0,5 ml 45,15 € Bromphenol blue sodium salt for electrophoresis Staining agent for visualisation of running distance in gel electrophoresis. BPB, water soluble C 19 H 9 Br 4 O 5 SNa M 691,9 g/mol A512.1 10 g glass 44,70 € Xylene cyanole (C.I. 42135) for histology and electrophoresis Staining agent for visualisation of running distance in gel electrophoresis. C 25H 27N 2NaO 6S 2 M 538,6 g/mol g Warning H319 WGK: 2 A513.1 10 g glass 46,10 € Orange G (C.I.16230) for microscopy and electrophoresis Acid Orange 10 C 16 H 10 N 2 Na 2 O 7 S 2 M 452,37 g/mol Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 RNAse-free 0318.1 10 g glass 10,50 € 0318.2 25 g plastic 15,35 € 861 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Markers Protein-Markers Roti ® -Mark All Roti ® -Mark size markers are supplied as ready-to-use solution, which may be applied directly to the gel. Marker STANDARD 10-150 10-150 PLUS PRESTAINED BICOLOR WESTERN Art. No. T851 (fig. 1) T850 (fig. 2) X879 (fig. 3) T852 (fig. 4) 2806 (fig. 5) 0948 (fig. 6) Staining unstained unstained 1 prestained band (30 kD) prestained two-colour prestained unstained Detection in Western-Blot no detection detection by anti-His detection by anti-His no detection no detection detection by conjugate 0949 Size Range (kD) 14.1 - 212 10 - 150 10 - 150 17 - 245 17 - 245 10 - 120 Number of Fragments 7 8 9 7 9 8 Number of Lanes/ml in Mini Gels approx. 200 approx. 200 approx. 200 approx. 200 approx. 200 approx. 200 Protein marker, not prestained Size range: 14-212 kD Number of fragments: 7 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Roti ® -Mark STANDARD is an ideal protein-size marker for routine applications. Proteins are solubilised in nonreducing buffer acc. to Laemmli. Fig.: 12 % SDS-PAGE (Tris) 862 (kD) - 212 - 118 - 66 - 43 - 29 - 20 - 14 (kD) - 150 - 100 - 80 - 60 - 40 - 30 - 20 - 10 Roti ® -Mark STANDARD ➀ TIP TTTIIPP (kD) - 150 - 100 - 80 - 60 - 40 - 31 blue - 30 - 20 - 10 (kD) - 245 - 123 - 95 - 50 - 33 - 23 - 17 T851.3 200 μl 25,80 € T851.1 1 ml 71,50 € T851.2 4 x 1 ml 252,65 € (kD) - 245 - 123 - 95 - 55 - 50 - 33 - 25 - 23 - 17 Roti ® -Mark 10-150 ➁ (kD) - 120 - 80 - 60 - 50 - 40 - 30 - 20 - 10 (kD) 120 - 80 - 60 - 50 - 40 - ➀ ➁ ➂ ➃ ➄ ➅ ➆ re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 30 - 20 - 10 - re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use TIP TTTIIPP (kD) - 120 - 80 - 60 - 50 - 40 - 30 - 20 - 10 Protein marker, not prestained Size range: 10-150 kD Number of fragments: 8 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Roti ® -Mark 10-150 consists of a set of genetically engineered proteins with defined, regular molecular weight. The proteins are not glycosylated and therefore display homogeneous electrophoretic behaviour. An enhanced 40 kD band provides easy orientation in the gel. Roti ® -Mark 10-150 has been assembled from a set of His-tagged, recombinant proteins that may be detected during Western-Blot analysis using anti-His antibodies. Fig.: 4-20 % gradient SDS-PAGE (Tris) T850.2 100 μl 36,35 € T850.1 500 μl 88,15 €
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Electrophoresis

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