Electrophoresis

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Electrophoresis · Gel Buffer Solutions Electrophoresis Gel Buffer Solutions Time sparing ready-to-use buffers saving valuable time and significantly reducing your effort to prepare electrophoresis buffers. Each batch is manufactured with reagents of the highest purity and is subsequently filtered. For gel electrophoresis and molecular biology. Rotiphorese ® 10x SDS-PAGE 10x concentrated SDS-PAGE running buffer, for electrophoresis. Solution consisting of 0.25 M Tris, 1.92 M glycine and 1 % (g/v) SDS in destilled, deionised water for protein analysis. Acc. to Laemmli (1970), Nature 227:680. 3060.1 1 l glass 29,50 € Original pack 6 x 1 l 28,03/1 l 3060.2 5 l plastic 78,05 € Original pack 2 x 5 l 74,15/5 l Rotiphorese ® 50x TAE Buffer Buffer concentrate for DNA electrophoresis. Store at +20 °C g Warning H315-H319 50-times concentrated running buffer, containing 2 M Tris, 1 M acetic acid and 50 mM EDTA in distilled, deionised water; pH 8.5. Mix before use! CL86.1 1 l glass 48,95 € Original pack 6 x 1 l 46,50/1 l CL86.2 5 l glass 149,45 € Original pack 2 x 5 l 141,98/5 l Rotiphorese ® 10x TAE Buffer new size re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see e yy readyto-use Buffer concentrate for DNA electrophoresis. Store at +20 °C 10-times concentrated running buffer, containing 0.4 M Tris, 0.2 M acetic acid and 10 mM EDTA in distilled, deionised water; pH 8.3. Mix before use! T845.1 1 l glass 22,05 € Original pack 6 x 1 l 20,95/1 l T845.2 5 l plastic 58,95 € Original pack 2 x 5 l 56,00/5 l Rotiphorese ® 10x TAE Buffer light with reduced EDTA concentration Buffer concentrate for DNA electrophoresis, ideal for DNA elution gels or in-gel-applications. Store at +20 °C Rotiphorese ® 10x TAE-buffer light is particularly ideal for agarose gels in DNA-elution. Although the reduced EDTA-content lowers the contamination of the eluted DNA with EDTA and therefore prevents any interference of enzymatic activity, e.g. from ligases, isomerases or polymerases, the buffer properties still remain totally intact. Particularly suitable for in-gel-applications when used with Rotigarose ® Low Melt (Art. No. 6351). 10-times concentrated serial buffer, containing 0.4 M Tris-acetate and 1 mM EDTA in distilled, deionised water; pH 8.3. Mix before use! 0122.1 1 l glass 22,05 € Original pack 6 x 1 l 20,95/1 l Rotiphorese ® 10x TBE Buffer Buffer concentrate, ideal for the preparation of sequencing gels and as running buffer in electrophoresis. Store at +20 °C n Danger H360 10-times concentrated buffer, containing 1.0 M Tris-Borate pH 8.3 and 20 mM EDTA in distilled, deionised water; pH 8.3. Suitable for manufacturing sequencing gels with Rotiphorese ® Gel 40 and as a running buffer. Mix before use! 3061.1 1 l glass 22,60 € Original pack 6 x 1 l 21,47/1 l 3061.2 5 l plastic 74,20 € Original pack 2 x 5 l 70,49/5 l Rotiphorese ® NF 10 x TBE Buffer Ready-to-use 10x TBE-buffer for preparing sequencing gels, also tested and optimised as running buffer in electrophoresis for automatic sequencers, fluorescence-free. Store at +20 °C 10-times concentrated buffer formulated specially for automated sequencing. 10x stock solution contains 0.89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8.3. Suitable for manufacturing sequencing gels with Rotiphorese ® -NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer. Mix before use! A118.1 2,5 l glass 84,95 € Original pack 4 x 2,5 l 80,70/2,5 l Concerning the Rotiphorese ® buffers 10x TAE, 50x TAE and 10x TBE During prolonged storage salt crystals may precipitate, which are easily resolubilised by gentle heating and shaking. The quality of the buffer is not affected by this procedure. Do not take out buffer solution when any precipitate is visible. Please note our Technical Information Brochure on the Internet (Life Science Main Page): Gels made of Polyacrylamide General technical informations Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 859 Elektrophorese
Elektrophorese Electrophoresis · Gel Loading Buffers Gel Loading Buffers for Proteins Roti ® -Load 1 (reducing) and Roti ® -Load 2 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by both buffer systems. Roti ® -Load 1 and Roti ® -Load 2 are 4x concentrated ready-to-use solutions, which may be used directly for sample loading. Modified formulation acc. to Laemmli (Nature 227, 1970). Roti ® -Load 1 reducing, 4x conc. Protein gel loading buffer. Store at +15-25 °C i j Danger H311-H331-H302-H315-H411 K929.1 10 ml glass 15,00 € K929.2 4 x 10 ml glass 44,00 € Roti ® -Load 2 non reducing, 4x conc. Protein gel loading buffer. Store at +20 °C K930.1 10 ml glass 12,80 € Summary Gel Loading Buffers for Nucleic Acids Art. No. Product Conc. Dyes Pack Qty. Fig. X905.1 Roti ® -Load DNA (with ficoll) 6x BP, XC 5x1.8 ml ➀ X904.1 Roti ® -Load DNA (with glycerol) 6x BP, XC 5x1.8 ml ➀ 0100.1 Roti ® -Load DNA 1x (with glycerol) 1x BP, XC 5x1 ml ➀ T847.1 Roti ® -Load DNA (with saccharose) 6x BP 5x1 ml ➁ 0095.1 Roti ® -Load DNA-short-run (with glycerol) 6x BP 5x1.8 ml ➁ 0099.1 Roti ® -Load DNA-short-run 1x 1x BP 5x1 ml ➁ 860 (with glycerol) re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see e yy readyto-use HP03.1 Roti ® -Load DNA-small (with glycerol) 6x XC 5x1.8 ml ➂ HP06.1 Roti ® -Load DNA-tricolor (with glycerol) 6x BP, XC, OG 5x1.8 ml ➃ HP05.1 Roti ® -Load DNA-orange 2 (with glycerol) 6x BP, OG 5x1.8ml ➄ HP04.1 Roti ® -Load DNA-orange 1 (with glycerol) 6x OG 5x1.8 ml ➅ T848.1 Roti ® -Load RNA 1,3x BP 2x0.5 ml ➁ BP = Bromphenolblau XC = Xylencyanol OG = Orange G Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Gel Loading Buffers for Nucleic Acids Roti ® -Load DNA and Roti ® -Load RNA are special gel loading buffers for nucleic acid electrophoresis, used for increasing the density when loading the gel. Roti ® -Load DNA is available in three different variations (with saccharose, glycerol or ficoll). We use Roti ® -Load DNA with glycerol (0100.1) or the specially designed short-run-loading buffer with glycerol (0099.1) for our DNA-markers. All gel loading buffers are ready-to-use and can be applied directly. Roti ® -Load DNA (with saccharose) DNase-free 6x gel loading buffer for DNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. Each batch is tested for its functionality in electrophoresis and proved to be DNase-free. The 6x loading buffer contains saccharose to increase the density and bromophenol blue as a staining agent to mark the run (see fig. 1). T847.1 5 x 1 ml 45,15 € Roti ® -Load DNA (with ficoll) DNase-free 6x gel loading buffer for DNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. Each batch is tested for its functionality in electrophoresis and proved to be DNase-free. The 6x loading buffer contains: Tris, Na-acetate, EDTA, bromophenol blue, xylene cyanol blue and ficoll (see fig. 1). X905.1 5 x 1,8 ml 33,80 € Roti ® -Load DNA (with glycerol) DNase-free 6x gel loading buffer for DNA gel electrophoresis. Store at -20 °C Transport temp.: room temp. Each batch is tested for its functionality in electrophoresis and proved to be DNase-free. The 6x loading buffer contains: Tris, Na-acetate, EDTA, bromophenol blue, xylene cyanol blue and glycerol (see fig. 1). X904.1 5 x 1,8 ml 30,65 € Safety data sheets can be found on the Internet next to the product description. DNAse-free re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see e yy readyto-use
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Electrophoresis

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