Electrophoresis

Electrophoresis · PAGE Precast Gels
Roti ® -PAGE Precast Gels
ready-to-use, for rapid and
easy protein gel electrophoresis
• Sharp resolution
• Very easy application
• Compatible with most minigels chambers
• Shelf life: 12 weeks from date of manufacture
• Well tested Rotiphorese ® quality
• Suitable for denaturing and native electrophoresis
• Consistent lot-to-lot and get-to-gel performance
• In-process controlled manufacturing and functional testing of every lot
• Well suited and optimised for all standard down-stream applications, like, e.g. gel staining (Coomassie,
fluorescence, silver), gel drying (vacuum, gel drying frames) and Western-Blotting (Semi-Dry, Tank)
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Gel parameters:
Cassette size (LxB): 9 x 10 and 10 x 10 cm
Gel size (LxB): 7.1 x 8.3 bzw. 8.1 x 8.3 cm
Gel thickness: 1 mm
Gel matrix continuous (10 % or 12 % polyacrylamide) or
in gradient (4 - 20 %)
Stacking gel 4 % polyacrylamide
Buffer system Tris/Glycine without SDS
Package contains Tris/Glycin buffer with 0.02 % sodium azide
Precast mini gels for rapid and easy gel electrophoresis
in laboratory and students courses.
Roti ® -PAGE mini gels come precast and are
very easy and quick to use.
Roti ® -PAGE with 12 % gel matrix or gradient gel matrix (4-20 %)
Coomassie Staining. Denaturing gel run in Tris/Glycin buffer with 1 % SDS.
1 hour, 25 mA (150 V, ramping to 300 V).
Lane 1-2: 8 µl Roti ® -Mark STANDARD (Art. No. T851);
Lane 3-4: 5 µl Roti ® -Mark PRESTAINED (Art. No. T852);
Lane 5-6: 5 µl Roti ® -Mark BICOLOR (Art. No. 2806);
Lane 7-8: 7,5 µl Roti ® -Mark 10-150 (Art. No. T850);
Lane 9-10: 7,5 µl Roti ® -Mark WESTERN (Art. No. 0948).
Our proven and tested Rotiphorese ® quality and the standardised tris-glycine buffer system are compatible with all running and
loading buffers according to Lämmli and all downstream applications (Western Blot, MS-analysis, sequencing etc.).
All Roti ® -PAGE Minigels include 4 % polyacrylamide stacking gels and yield a sharp band separation in the recommended protein range.
Because of their variable format of 9 x 10 cm or 10 x 10 cm, the gel plates fit all standard mini-electrophoresis chambers and can be
separated easily after the run without damaging the precious gel. The gel matrix contains no SDS (for native gels), a running buffer
containing SDS is recommended for denaturing gels (e.g. Rotiphorese ® 10x SDS-PAGE, Art. No. 3060).
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
Store at +4 °C
Transport temp.: cooled
Gel plates may be separated very easily
by using the comb without tearing the
precious gel.
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Elektrophorese