Electrophoresis

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<strong>Electrophoresis</strong> · Agarose and Gelling bp 12000 - 7000 - 4000 - 3000 - 2000 - 1650 - 1000 - 500 - 100 - agarose in 0.5 x TBE 0.7 % 1.0 % 1.3 % Agarose Low Melt Roti ® garose particularly for gel elution and in-gel-application • Low melting point and low gelling temperature • Particular high transparency and very low self fluorescence. For particular clear gels. • The ideal solution when nucleic acid fragments or proteins are to be recovered after electrophoretic resolution • Suitable for enzymatic reactions in liquid agarose (in-gel-applications) • Excellent for immobilising microorganisms and virus-plaque-assays • Suitable for cell culture applications (animal and plant) • Tested for stability after autoclaving Guarantee analysis: Electroendosmosis (EEO). ..................................................................................≤0,12 Water content. ......................................................................................................≤7 % Ash. ...................................................................................................................≤0,4 % Sulphate (SO4). ...............................................................................................≤0,12 % Gel strength (1.5 % gel). .............................................................................≥500 g/cm2 Gelling temperature (1.5 % gel)................................................................. 24-28 °C Melting temperature (1.5 % gel). ............................................................... ≤65,5 °C Turbidity (1.5 % solution). ................................................................................ ≤4 NTU DNases, RNases. .................................................................................. none detected 6351.1 5 g plastic 30,95 € 6351.5 25 g plastic 129,00 € 6351.2 100 g plastic 448,30 € 6351.4 500 g plastic 1907,05 € Original pack 6 x 500 g 1811,70/500 g Agarose MEEO ultra-quality new size bp - 2000 - 1500 - 1000 - 750 - 500 - 250 DNAse-free RNAse-free Roti ® garose for electrophoresis Medium electroendosmosis (EEO = 0.16-0.19) • Special agarose for immuno, serum and antibody electrophoresis • Suitable for separation of nucleic acids between 500 bp and 10 kb • Gels are well-to-handle and have a strong matrix, even when using thin gels of 1-2 mm 2268.1 10 g plastic 16,80 € 2268.2 100 g plastic 91,85 € 2268.4 500 g plastic 405,30 € Original pack 6 x 500 g 385,04/500 g 2268.3 1 kg plastic 661,15 € Original pack 6 x 1 kg 628,09/1 kg BIG BIG PACK PACK PACK 3 % agarose in 1 x TBE bp bp 1353 - 872 - 603 - 310 - 194 - 118 - 72 - Agarose LM/PCR Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 - 622 - 404 - 240 - 160 - 110 - 67 DNAse-free RNAse-free Roti ® garose particularly for gel elution of fragments under 1500 bp in low melting agarose High resolution low melting agarose in genetic engineering quality. For optimal fragment separation, DNA recovery and cloning of PCR amplificates. • Fine resolution of fragments from 50 to 1500 bp • Very well suited for analysis and recovery of PCR amplificates and fragments derived from restriction digests • Also well suitable for in-gel-applications • Low gel background, compatible with all standard nucleic acid-staining methods • Low viscosity agarose, recommended for use in concentrations of 3 to 6 % agarose. Note: gels of 2 % agarose and lower are fragile Guarantee analysis: Electroendosmosis (EEO). ..................................................................................≤0,13 Water content. ......................................................................................................≤7 % Ash. .................................................................................................................≤0,45 % Sulphate (SO4). ...............................................................................................≤0,12 % Gel strength (4 % gel). ................................................................................≥800 g/cm2 Gelling temperature (4 % gel)........................................................................≤35 °C Melting temperature (4 % gel). ......................................................................≤65 °C Turbidity (4 % solution). ................................................................................... ≤6 NTU DNases, RNases. .................................................................................. none detected DNA binding....................................................................................... none detected In-gel-processing. ...................................................................................... complies Agarase digest. .......................................................................................... complies HP31.1 5 g plastic 33,20 € HP31.2 100 g plastic 464,40 € HP31.3 250 g plastic 985,80 € Guarantee analysis: Electroendosmosis (EEO). ............................................................................0,16-0,19 Water content. ......................................................................................................≤7 % Ash. ...................................................................................................................≤0,5 % Sulphate (SO 4 ). ...............................................................................................≤0,14 % Gel strength (1 % gel). ..............................................................................≥1000 g/cm 2 Gel strength (1.5 % gel). ...........................................................................≥2200 g/cm 2 Gelling temperature (1.5 % gel). .................................................................. 36 ±1,5 °C Melting temperature (1.5 % gel)................................................................... 88 ±1,5 °C Turbidity (1.5 % solution). ................................................................................ ≤4 NTU 857 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Agarose and Gelling 2180 - 1030 - Agarose Super LM Roti ® garose for gel elution from melted agarose, capillary electrophoresis and in-gel-processing Low melting agarose with particularly low gelling and melting temperatures. For DNA recovery of fragments over 1000 bp and for special applications. • Fine resolution of fragments over 1000 bp • Very well suited for analysis and recovery of longer PCR amplificates or other longer fragments like vectors, rare cutter digestions etc. • Recommended for in-gel-applications, virus plaque-assays and tissue/cell culture applications • Low gel background, compatible with all standard nucleic acid-staining methods • Lower gel strength than Agarose Low Melt. Recommended for use in concentrations of over 1 % agarose Guarantee analysis: Electroendosmosis (EEO). ..................................................................................≤0,12 Water content. ......................................................................................................≤7 % Ash. .................................................................................................................≤0,35 % Sulphate (SO4). ...............................................................................................≤0,14 % Gel strength (1.5 % gel). .............................................................................≥300 g/cm2 Gelling temperature (1.5 % gel).....................................................................≤20 °C Melting temperature (1.5 % gel). ...................................................................≤62 °C Turbidity (1.5 % solution). ................................................................................ ≤4 NTU DNases, RNases. .................................................................................. none detected HP45.1 5 g plastic 33,20 € HP45.2 100 g plastic 464,40 € HP45.3 250 g plastic 985,80 € Agarose HEEO ultra-quality Roti ® garose for electrophoresis High electroendosmosis (EEO = 0.23-0.26) • Special agarose for protein separation and countercurrent electrophoresis • Gels are well-to-handle and have a strong matrix, even when using thin gels of 1-2 mm • Very clear and transparent matrix, even for thick gels Guarantee analysis: Electroendosmosis (EEO). ............................................................................0,23-0,26 Water content. ......................................................................................................≤7 % Ash. ...................................................................................................................≤1,0 % Sulphate (SO 4). .................................................................................................≤0,2 % Gel strength (1 % gel). ................................................................................≥750 g/cm 2 Gel strength (1.5 % gel). ...........................................................................≥1200 g/cm 2 Gelling temperature (1.5 % gel). ..................................................................36 ±1,5 °C Melting temperature (1.5 % gel)...................................................................88 ±1,5 °C Turbidity (1.5 % solution). ................................................................................ ≤4 NTU 2269.1 10 g plastic 17,65 € 2269.2 100 g plastic 97,35 € 2269.3 1 kg plastic 708,45 € Original pack 6 x 1 kg 673,03/1 kg 858 1,8 % Agarose in 1 x TAE bp bp 590 - 430 - 270 - 184 - 124 - - 1500 - 1000 - 500 - 300 - 100 BIG BIG PACK PACK PACK DNAse-free RNAse-free bp 3700 - 1300 - 622 - 527 - 404 - 309 - 240 - 160 - 123 - 1.5 % Synergel TM + 0.7 % agarose Synergel ≥99 % For gel electrophoresis. Agarose additive Store at +20 °C TM • Enhances separation of nucleic acid fragments • For extremely clear gels Synergel TM is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system. The addition of Synergel TM to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0.7 % agarose and 1.0 % Synergel TM , e.g., will produce improved results when compared to a 2 % agarose-only gel. Synergel TM also provides greater optical clarity, which allows higher quality photodocumentation of stained gels. Figure 1 compares separation qualities of A: 1.5 % SynergelTM / 0.7 % agarose and B: 4 % high-resolution agarose (NuSieve ® ). Please see also figures for pUC19 markers T149 and X901. Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel. RNA may also be separated in this system by using a 2.2 M formaldehyde-containing buffer. Nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols. Type analysis: Colour............................................................................................................. off-white Odour. .......................................................................................................slightly salty pH (1 % solution). .......................................................................................................9 DNase, RNase....................................................................................... none detected 0184.2 10 g plastic 24,35 € 0184.1 100 g plastic 170,95 € How to Calculate Required Synergel TM Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 4 % agarose A B 1. What percentage agarose gel is currently being used? 2. The gel will inevitably contain 0.7 % agarose (the minimum agarose concentration required to ensure gel stability). Hence, subtract 0.7 % from the agarose total. 3. Divide the difference by 2. The result is the percentage of Synergel TM to be added to the 0.7 % agarose. DNAse-free RNAse-free Example: Convert a 1 % agarose to a 1 % SynergelTM /agarose gel a. Substract 0.7 % from the 1 % agarose: 1 % - 0.7 % = 0.3 % b. Divide the difference by 2: 0.3 % : 2 = 0.15 % c. 0.15 % (150 mg / 100 ml) is the amount of SynergelTM added to 0.7 % agarose (0.7 g / 100 ml) to produce the functional equivalent of a 1 % agarose gel.
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Electrophoresis

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