Electrophoresis

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<strong>Electrophoresis</strong> · PAGE Reagents Rotiphorese ® ready-made solutions for manual sequencing • For preparing 4-20 % sequencing gels • No tedious dissolving of urea • Prevents acrylamide dusts • Simple handling re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see e yy readyto-use Rotiphorese ® DNA sequencing system Store at +8 °C g n Danger H340-H350-H372-H361f-H302-H315-H319-H317 The complete system at a low price contains: • 1 l Rotiphorese ® sequencing gel concentrate (3043.1) • 1 l Rotiphorese ® sequencing gel diluent (3047.1) • 250 ml Rotiphorese ® sequencing gel buffer concentrate (3050.1) Preparation of gel • Mix the three components (Sequencing concentrate + Sequencing diluent + Buffer concentrate) • Add APS and TEMED • Cast the gel A431.1 1 kit 87,65 € Rotiphorese ® Sequencing gel concentrate Ready-to-use solution (19:1) for the preparation of gels containing 0-20 % monomer, for electrophoresis. Also part of the DNA sequencing system. Store at +8 °C n g Danger H340-H350-H372-H361f-H302-H315-H319-H317 Ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1. For the preparation of polyacrylamide sequencing gels containing 0-20 % monomer; 23.75 % acrylamide,1.25 % methylene bisacrylamide and 50 % urea (8.3 M). 3043.2 100 ml glass 11,20 € 3043.1 1 l glass 49,95 € Original pack 6 x 1 l 47,45/1 l Rotiphorese ® Sequencing gel diluent 50 % (g/v; 8.3 M) solution of urea in water for diluting Rotiphorese ® Sequencing gel concentrate. Store at 2-15 °C 3047.1 1 l glass 31,15 € Original pack 6 x 1 l 29,59/1 l Rotiphorese ® Sequencing gel buffer concentrate 50 % (g/v; 8.3 M) solution of urea in 10x TBE (1 M Tris-borate, 20 mM EDTA buffer, pH 8.3), for electrophoresis. Store at +8 °C 3050.1 250 ml glass 20,35 € Reagents for Polyacrylic Amide Gels Acrylamide, 2 x cryst. ≥98 %, 2x cryst., extra pure For gel electrophoresis Propene amide, acrylic acid amide C 3 H 5 NO M 71,08 g/mol Store at +4 °C i n Danger H301-H340-H350-H372-H361f-H312-H332-H315-H319-H317 Standard acrylamide, inexpensive and versatile. For all standard applications. 7871.1 500 g plastic 37,50 € Original pack 6 x 500 g 35,63/500 g 7871.2 1 kg plastic 47,95 € Original pack 6 x 1 kg 45,55/1 kg Acrylamide, 4 x cryst. ≥99.9 %, p.a., 4x cryst. For gel electrophoresis. Propene amide, acrylic acid amide C 3 H 5 NO M 71,08 g/mol Store at +4 °C i n Danger H301-H340-H350-H372-H361f-H312-H332-H315-H319-H317 Top-quality acrylamide, particularly suitable for sequencing gels and flourescence labelled applications. 7906.1 100 g plastic 26,55 € 7906.2 1 kg plastic 188,15 € Original pack 6 x 1 kg 178,74/1 kg N,N'-Methylene bisacrylamide ≥98 %, for gel electrophoresis Bis, Bisacrylamide C 7H 10N 2O 2 M 154,17 g/mol Store at +4 °C g Warning H302 7867.1 50 g plastic 24,40 € 7867.2 100 g plastic 37,95 € Urea ≥99.5 %, p.a., ultra quality For molecular biology and biochemistry. Carbamide CH 4 N 2 O M 60,06 g/mol Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 2317.3 500 g plastic 22,60 € Original pack 6 x 500 g 21,47/500 g 2317.1 1 kg plastic 38,95 € Original pack 6 x 1 kg 37,00/1 kg 2317.2 5 kg plastic 148,35 € BIG BIG PACK PACK PACK Safety data sheets can be found on the Internet next to the product description. 851 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Agarose and Gelling Rotiphorese ® NF-Urea tested for automatic sequencers, fluorescence free CH 4 N 2 O M 60,06 g/mol Store at +4 °C A120.1 1 kg plastic 48,60 € Original pack 6 x 1 kg 46,17/1 kg APS ≥98 %, p.a., ACS Oxidizing agent for Cu, for separation of Mn and Cr. For electrophoresis. Polymerization catalyst. Ammonium persulphate, Ammonium peroxydisulphate n o g Danger H334-H272-H302-H335-H315-H319-H317 Polymerisation catalyst for acrylamide/ bisacrylamide polymerisation. Use 10 % APS solution - fill 1 g of APS with water to an end volume of 10 ml. Prepare freshly or store at -20 °C. Freeze and thaw infrequently. 9592.3 50 g plastic 7,55 € 9592.2 100 g plastic 10,25 € 9592.1 1 kg plastic 27,75 € Original pack 6 x 1 kg 26,36/1 kg 9592.4 2,5 kg plastic 52,15 € TEMED 99 %, p.a, for electrophoresis N,N,N',N'-Tetramethylethylenediamine, 1,2-Bis(dimethylamino)-ethane l h g Danger H225-H314-H302-H332 Catalyst for acrylamide polymerisation, filled under nitrogen. Working concentration, e.g. when preparing sequencing gels: 40 µl TEMED for 100 ml gel casting solution. 2367.3 25 ml glass 13,45 € 2367.1 100 ml glass 27,90 € 2367.2 250 ml glass 55,90 € Roti ® -Stock 20 % SDS ready-to-use, 0.2 µm filtered For molecular biology and electrophoresis. SDS 20 % ready-to-use solution g Warning H315-H319 20 % stock solution sodium dodecyl sulphate. Store the solution away from light in a dry place. SDS-stock solution and other solutions containing SDS should not be cooled below 24 °C otherwise the SDS may precipitate. The crystals can be easily resolubilised by heating them between 30 to 50 °C. With original seal. Composition: 20 % SDS in deionised water, 0.2 µm filtered 1057.1 1 l glass 107,40 € Original pack 6 x 1 l 102,03/1 l 852 re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use S Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Roti ® garoses and Gelling Reagents Roti ® garose is a neutral polysaccharide which is obtained in many purification steps from the cell wall of certain algae and red algae. Roti ® garose forms very clear gels with all standard running buffers and will result in a sharp and clear separation of your biomolecules. Extremely pure agarose with very low interference binding to staining reagents which produces a low background and high contrast appearence after staining. • For clear and sharp bands • Gels with high transparency • Low background • Suitable for all standard running buffers and high-speed buffer systems • Compatible with all nucleic acid staining systems • Non-toxic in cell immobilisation assays • Of course DNase and RNase free Roth has the right agarose for each application: Roti ® garose Art. No. Application Standard 3810 Routine gels, student´s courses, general analyses (1-20 kb). NEEO ultra quality 2267 All standard applications, qualitative and quantitative gels, screening and blotting. Agarose Tablets HP67 Highly reproducible gels, or simple applications in students courses. Suitable for all standard-gels (0.5-0.25 %). GTQ 6352 Genetic engineering quality, for DNA-elution of fragments >1000 bp without melting the agarose* For the total analytical range (200 bp up to 40 kb), Broad Range T846 Pulse-Field electrophoresis, blotting, shift assays. Ideal when only a few agaroses are to be used in the laboratory. Pulsed-Field 3771 Separation of large fragments (from 20 kb up), Pulsed Field gel electrophoresis (PFGE). HR PLUS HP30 Analysis of fragments between 100 and 3000 bp. High Resolution K297 Analysis of fragments between 50 and 1000 bp. Low Melt 6351 With low melting and gelling temperatures (MT 65.5 °C, GT 24-28 °C). For gel elution from melted agarose. Genetic engineering quality with low melting and gelling LM / PCR HP31 temperatures (MT
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Electrophoresis

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