Electrophoresis

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<strong>Electrophoresis</strong> · Markers (bp) - 5000 - 4000 - 3000 - 2500 - 2000 - 1500 - 1000 - 900 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 150 - 100 (bp) - 8000 - 6000 - 5000 - 4000 - 3000 - 2500 - 2000 - 1750 - 1500 - 1250 - 1000 - 750 - 500 - 250 DNA-ladder + gel loading buffer Size range: 100-5000 bp Number of fragments: 17 Number of lanes per 100 µg: approx. 160 (mini gels) Store at -20 °C Transport temp.: room temp. Fragment sizes 100 to 1000 bp are equimolar. Fig.: 1.8 % agarose (NEEO, Art. No. 2267) The DNA-ladder is supplied in lyophilised form. Sample buffer for dissolving the lyophilised DNA-fragments is included. DNA-ladder + gel loading buffer Size range: 250-8000 bp Number of fragments: 16 Number of lanes per 100 µg: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Universal marker for size analysis of plasmids and DNA-insertions. Two fragments of 1500 and 2500 bp have been reinforced for easy orientation in the gel. The DNA amount per band has been optimised in such a way that a small running distance and low amounts of marker are sufficient for optimum separation. Fig.: 1.8 % agarose (NEEO, Art. No. 2267) The ladder is supplied in lyophilised form. Sample buffer for dissolving the lyophilised DNA-fragments is included. (bp) - 10000 - 8000 - 6000 - 5000 - 4000 - 3000 - 2500 - 2000 - 1500 - 1000 ➄ ➅ ➆ ➇ 100 bp-DNA-Ladder extended ➄ T835.1 50 μg + gel loading buffer 60,40 € T835.2 4 x 50 μg + gel loading buffer 223,05 € 250 bp-DNA-Ladder ➅ T918.1 50 μg + gel loading buffer 63,40 € Instructions for use can be found on the Internet next to the product description. - 500 1 kbp DNA-Ladder ➆ (bp) - 10000 - 3000 - 2000 - 1500 - 1000 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 150 - 100 DNA-ladder + gel loading buffer Size range: 50-10000 bp Number of fragments: 11 Number of lanes per 100 µg: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Universal marker for electrophoretic separation of fragments between 500 bp and 10 kb, optimally suited for size determination of inserts, plasmids, and restricted DNA. The mass of the larger fragments was reduced, allowing to obtain clear and distinct bands with short running distances even for the large marker fragments. The terminal sticky EcoRI-restriction sites allow simple labelling of the marker fragments via end-labelling techniques. The 1 kbp DNA-ladder is highly compatible with the 100 bp DNA-ladder (equalised: T833.1). Fig.: 1.2 % agarose (NEEO, Art. No. 2267). Additional band at 125 bp. The DNA-ladder is supplied in lyophilised form. Sample buffer for dissolving the lyophilised DNA-fragments is included. Y014.1 50 μg + gel loading buffer 28,90 € Y014.2 4 x 50 μg + gel loading buffer 100,05 € DNA-Ladder combi ➇ Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 DNA marker + gel loading buffer Size range: 100-10000 bp Number of fragments 20 Number of lanes per 40 µg + 100 µg: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Versatile DNA-marker combination for gels and DNA-fragments of different size. DNA-ladder combi combines the advantages of the 100 bp ladder, equalized, with the versatile 1 kbp DNA-ladder. Subsequently we now have a marker set that completely covers both the medium size range up to 10 kb (see figure of Y014) as well as enabling clear size analysis in the small fragment range. The two markers come in separate tubes to ensure high flexibility in the laboratory. They can, therefore, be used separately or combined. The bands below 500 bp were enhanced aligning their intensity in the gel to the large fragments. The 500 bp band was specially enhanced for easy orientation in the gel. The figure shows a gel mixture of 0.9 % Agarose + 0.5 % Synergel ® with (left to right): 0,4 µg 100 bp Ladder, equalized + 0.3 µg 1 kbp Ladder; 0.5 µg 1 kbp Ladder, 0.4 µg 100 bp Ladder, equalized. The DNA-ladder is supplied in lyophilised form. Sample buffer for dissolving the lyophilised DNA-fragments is included. CL22.1 20 μg + 50 μg + gel loading buffer 86,00 € 867 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Markers DNA Markers for Short Runs Marker PCR-Marker DNAscore DNA-Marker short run 1 DNA-Marker short run extended DNA-Marker ccc-Plasmid Art. No. T917 (fig. 1) 0146 (fig. 2) CL05 (fig. 3) 0149 (fig. 4) Size range (bp) 500 - 1500 500 - 2500 100 - 6000 3000 - 12000 Number of fragments 7 5 9 3 Reinforced bands at (bp) 500, 1000, 1500 1500 1500 - Number of lanes in minigel 200 / 100 μg 400 / 100 μg 300 / 100 μg 1000 / 100 μg Delivery lyophilized lyophilized lyophilized lyophilized DNA marker + gel loading buffer Size range: 500-1500 bp Number of fragments: 7 Reinforced bands: 500 bp, 1000 bp, 1500 bp Band for DNA quantitation: 1000 bp Number of lanes per 100 µg: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. PCR-Marker DNAscore resolves extremely quickly and is therefore excellent for analysing PCR-fragments and for routine-diagnostics. The 500 bp, 1000 bp and 1500 bp bands have been reinforced in their intensity for speedy and simple orientation. The special 1000 bp band allows the marker to be used for quantifying in gel. Depending on the resolved segment, the marker changes its appearance (see figure): A) 1.0 cm: The marker shows three bands (500 bp, 1000 bp, 1500 bp). B) 1.5 cm: Four satellite bands flank the 1000-bp-band. C) 3 4 cm: All bands are resolved. Efficient size analysis of fragments with a difference of 50 bp is possible. D) 6-7 cm: The marker can also be used for quantfying after approx. 6 cm run: the 1000-bp-band corresponds to140 ng DNA (when loading 0.5 µg DNA-marker). DNA-marker + gel loading buffer Size range: 500-2500 bp Number of fragments: 5 Number of lanes per 100 µg: approx. 400 (mini gels) The perfect DNA-marker for very short gel running distances and high-through-put approaches. Store at -20 °C Transport temp.: room temp. Best results are already obtained at 18 mm running distance in a 1.5 % agarose gel (see figure). The fragment mass is optimised for very short runs. The 1500 bp fragment has been enhanced for easy analysis of the gel. Fig.: 1.5 % agarose (NEEO, Art. No.2267). Left - running distance 18 mm, Right - running distance 40 mm. The DNA-ladder is supplied in lyophilised form. A short-run-optimised sample buffer for dissolving the lyophilised DNA-fragments is included (Art. No. 0099.1). 868 A B C D (bp) - 1500 - 1200 - 1100 - 1000 - 900 - 800 - 500 (bp) 2500 - 2000 - 1500 - 1000 - 500 - (bp) - 2500 - 2000 - 1500 - 1000 - 500 (bp) 6000 - 1500 - (bp) - 6000 - 4000 - 2500 - 2000 - 1500 ➀ ➁ ➂ ➃ PCR-Marker DNAscore ➀ DNA-Marker short-run extended ➂ T917.1 50 μg + gel loading buffer 63,40 € DNA-Marker short-run 1 ➁ 0146.1 50 μg + gel loading buffer 55,45 € 100 - - 1000 - 500 - 200 - 100 DNA-Marker + gel loading buffer Size range: 100-6000 bp Number of fragments: 9 Number of lanes per 100 µg: approx. 300 (mini gels) Store at -20 °C Transport temp.: room temp. New DNA marker, optimised for all standard applications in the lab. Due to the lesser number of fragment bands, the short-run, extended DNA-Marker separates very efficiently even on very short running distances, making it a versatile tool also for high-throughput and short-term electrophoresis. Best results are already obtained at 15 mm running distance / 7 minutes runtime in a 1.5 % agarose gel with 0.3 µg DNA loading per lane (see figure). The fragment mass is optimised for very short runs and the enhanced 1500 bp fragment provides easy orientation for analysis. Fig.: Left - 1.5 % agarose (NEEO, Art. No. 2267), running distance 15 mm. Right - 1.2 % agarose, running distance 35 mm. The DNA-ladder is supplied in lyophilised form. A short-run-optimised sample buffer for dissolving the lyophilised DNA-fragments is included. DNA-marker + gel loading buffer Size range: 3000-12 000 bp Number of fragments: 3 Number of lanes per 100 µg: approx. 1000 (mini gels) A “covalently closed circle“ DNA-marker for rapid determination of plasmid size and estimation of plasmid concentration following DNA-preparation. Store at -20 °C Transport temp.: room temp. The excellent separation efficiency of the marker even on short running distances allows for the application in high-throughput approaches. Best results are already obtained at 20 mm running distance in a 1.2 % agarose gel (see figure). Fig.: 1.2 % agarose (NEEO, Art. No. 2267), running distance 20 mm. The DNA-ladder is supplied in lyophilised form. A short-run-optimised sample buffer for dissolving the lyophilised DNA-fragments is included. (bp) - 12000 - 6000 - 3000 CL05.1 50 μg + gel loading buffer 63,10 € DNA-Marker ccc-plasmid ➃ Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 0149.1 10 μg + gel loading buffer 48,70 €
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Electrophoresis

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