Electrophoresis

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<strong>Electrophoresis</strong> · Blotting Units Blotting Units Tank blotting • Modules fit the vertical electrophoresis system Rotiphorese ® PROclamp (see page 885) • For transferring proteins and nucleic acids • Ideal for large and temperature-sensitive proteins • The short distance between the electrodes guarantees an evenly high current strength across the blotting area and reduces the amount of heat generated Rotiphorese ® PROclamp MINI Tank Blotting System Blotting area: 10 x 10 cm (per cassette) • Electro tank blotting system for blotting of up to 4 gels in parallel • Can be used with all gels of 10 x 10 cm in maximum • Hinged cassettes – for most easy handling and rapid blot set up • Open design, rigid cassettes for maximum current transfer and even blotting contact • Colour coded cassettes and asymmetrical lid ensure correct blot set up and blotting direction • Compatible with the Rotiphorese ® PROclamp MINI unit for vertical electrophoresis Delivery incl. 1 Rotiphorese ® PROclamp MINI buffer tank, safety lid with cables and 1 PROclamp blotting module for max. 4 gels, incl. 4 cassettes and 4 pairs of fibre blotting mats. Art. No. Pack Qty. € 3513.1 1 unit 686,95 Rotiphorese ® PROclamp MINI Tank Blotting Module Blotting insert compatible with the Rotiphorese ® PROclamp MINI system for vertical electrophoresis (3501.1 - 3503.1). Blotting module for max. 4 gels, incl. 4 cassettes and 4 pairs of fibre blotting mats. Art. No. Pack Qty. € 3517.1 1 unit 419,25 Accessories: PROclamp compression cassette (no mats) 3518.1 1 unit 46,70 PROclamp blotting mats 3519.1 4 pairs 34,40 Semi Dry Blotters For transfer of proteins and nucleic acids. • Durable, corrosion-free electrodes • Fast, steady transfer • Minimum heat development • Low buffer use Electrodes made of platinized titanium (anode) and rust-free high-grade stainless steel (cathode). As the electrodes are made of high-grade steel and platinised titanium, they will last indefinitely if used correctly. There is no build-up of deposits on the transfer surface, the buffers cannot penetrate into the electrodes. The extremely constant electric field over the entire transfer surface guarantees optimal results with all transfer membranes. MINI Semi-dry-blotter, transfer surface 10 x 10 cm Art. No. Pack Qty. € T787.1 1 683,70 MAXI Semi-dry-blotter, transfer surface 20 x 20 cm Art. No. Pack Qty. € T788.1 1 1439,45 Capillary Blotter For Southern and Northern transfer. Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 New, optimised model! • Quick and easy assembly • DNA-transfer in two hours or less • RNA-transfer in three hours or less • Efficient transfer of high or low molecular fragments • Special lid construction for constant and steady pressure The construction enables constant and steady capillary suction without having to use conventional, overhanging filter papers. The construction absorbs a particularly large amount of liquid and enables a speedy and even transfer. Several gels can be blotted simultaneously. MAXI Capillary blotter, transfer surface 26 x 28 cm Art. No. Pack Qty. € N856.1 1 384,85 895 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Blotting Membranes Dot Blotter and Slot Blotter For rapid transfer of DNA, RNA and immunological antibody screening methods. • Robust acrylic construction • Precision lapped mating surfaces prevent sample leakage • Steady transfer • Very simple assembly • Alpha-numeric sample identification A permanent filter template is provided with each manifold to simplify the cutting of filters to exact size. A vacuum of approximately 600 mm Hg (0.8 Bar) is required during sample application. We recommend LABOPORT ® -Membrane vacuum pump (Art. No. Y371.1) and the following hoses: Rotilabo ® -Vacuum-Silicone hose, inner ∅ 8 mm (Art. No. 9746.1) or Rotilabo ® -Vacuum-Rubber hose, inner ∅ 8 mm (Art. No. 0680.1). Dot-blotter (A) Max. sample capacity: 96 per transfer Wells per row: 12 Wells per column: 8 Volume per well: approx. 180 µm (for shape of wells, see figure A.2) Type Art. No. Pack Qty. € Dot-Blotter T790.1 1 628,90 Slot-blotter (B) Max. sample capacity: 48 per transfer Wells per row: 16 Wells per column: 3 Volume per well: approx. 360 µm (for shape of wells, see figure B.2) Type Art. No. Pack Qty. € Slot-Blotter T791.1 1 671,90 Instructions for use can be found on the Internet next to the product description. 896 Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Transfer Filter Membranes Transfer Membranes made of PVDF For optimal results with all protein transfer techniques • Well suited for all protein blots • Best signal-to-noise ratio •Very robust • 0.2 µm or 0.45 µm pore size • Suitable for long-term exposition • Suitable for all detection systems • May be used for peptide sequencing Roti ® -Fluoro PVDF Premium PVDF membrane from ROTH, suitable for direct fluorescence detection Roti ® -Fluoro PVDF is a white, microporous membrane made from naturally hydrophobic polyvinylidene fluoride. The membrane is of superior protein binding capacity, making it perfectly suited for all standard and special applications in the field of protein analysis. Roti ® -Fluoro PVDF is of 0.2 µm pore size and binds proteins tenaciously via hydrophobic interactions. It enables very sensitive protein detection by keeping the background very low, resulting in a good signal-to-noise ratio with all standard detection systems. The mechanically rigid membrane is of high tensile strength and tear-proof, and does not crack or curl during handling. Therefore, signals remain clear and distinct, and are undisturbed by false-positive signals caused by membranes crinkles. Roti ® -Fluoro PVDF has been specially optimized for direct detection via fluorescence. Briefly immerse Roti ® -Fluoro PVDF membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every aqueous blot system. Applications: Western- and Far-Western-Blot, analysis of small peptides, Immunoblot, Dot-blot, binding assays, N-terminal sequencing Detection systems: Fluorescence, chemoluminescence, radioactivity, colour Pore size Binding Dimensions L x W Art. No. Pack Qty. € (μm) (cm) 0.2 hydrophobic 20 x 13 2831.1 2 sheets 32,05 0.2 hydrophobic 330 x 26 2803.1 1 roll 291,35
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Electrophoresis

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