Electrophoresis
Electrophoresis
Electrophoresis
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Elektrophorese<br />
<strong>Electrophoresis</strong> · Blotting Membranes<br />
Dot Blotter and Slot Blotter<br />
For rapid transfer of DNA, RNA and immunological antibody<br />
screening methods.<br />
• Robust acrylic construction<br />
• Precision lapped mating surfaces prevent sample leakage<br />
• Steady transfer<br />
• Very simple assembly<br />
• Alpha-numeric sample identification<br />
A permanent filter template is provided with each manifold to simplify the<br />
cutting of filters to exact size.<br />
A vacuum of approximately 600 mm Hg (0.8 Bar) is required during sample<br />
application. We recommend LABOPORT ® -Membrane vacuum pump<br />
(Art. No. Y371.1) and the following hoses:<br />
Rotilabo ® -Vacuum-Silicone hose, inner ∅ 8 mm (Art. No. 9746.1) or<br />
Rotilabo ® -Vacuum-Rubber hose, inner ∅ 8 mm (Art. No. 0680.1).<br />
Dot-blotter (A)<br />
Max. sample capacity: 96 per transfer<br />
Wells per row: 12<br />
Wells per column: 8<br />
Volume per well: approx. 180 µm (for shape of wells, see figure A.2)<br />
Type Art. No. Pack Qty. €<br />
Dot-Blotter T790.1 1 628,90<br />
Slot-blotter (B)<br />
Max. sample capacity: 48 per transfer<br />
Wells per row: 16<br />
Wells per column: 3<br />
Volume per well: approx. 360 µm (for shape of wells, see figure B.2)<br />
Type Art. No. Pack Qty. €<br />
Slot-Blotter T791.1 1 671,90<br />
Instructions for use<br />
can be found on the Internet next to the product description.<br />
896<br />
Global Step - Email : Office@global-step.net<br />
Telefon : (+4) 0742 095 539<br />
Tel/Fax : (+4) 031 104 85 87<br />
Mobil : (+4) 0720 095 539, 0723 442 593<br />
Transfer Filter Membranes<br />
Transfer Membranes made of PVDF<br />
For optimal results with all protein transfer techniques<br />
• Well suited for all protein blots<br />
• Best signal-to-noise ratio<br />
•Very robust<br />
• 0.2 µm or 0.45 µm pore size<br />
• Suitable for long-term exposition<br />
• Suitable for all detection systems<br />
• May be used for peptide sequencing<br />
Roti ® -Fluoro PVDF<br />
Premium PVDF membrane from ROTH, suitable for direct<br />
fluorescence detection<br />
Roti ® -Fluoro PVDF is a white, microporous membrane made from naturally<br />
hydrophobic polyvinylidene fluoride. The membrane is of superior protein<br />
binding capacity, making it perfectly suited for all standard and special<br />
applications in the field of protein analysis.<br />
Roti ® -Fluoro PVDF is of 0.2 µm pore size and binds proteins tenaciously via<br />
hydrophobic interactions. It enables very sensitive protein detection by<br />
keeping the background very low, resulting in a good signal-to-noise ratio<br />
with all standard detection systems. The mechanically rigid membrane is of<br />
high tensile strength and tear-proof, and does not crack or curl during<br />
handling. Therefore, signals remain clear and distinct, and are undisturbed<br />
by false-positive signals caused by membranes crinkles.<br />
Roti ® -Fluoro PVDF has been specially optimized for direct detection<br />
via fluorescence.<br />
Briefly immerse Roti ® -Fluoro PVDF membrane in 100 % methanol prior to<br />
transfer until no more white areas are visible. This transfer membrane is<br />
then compatible with every aqueous blot system.<br />
Applications:<br />
Western- and Far-Western-Blot, analysis of small peptides,<br />
Immunoblot, Dot-blot, binding assays, N-terminal sequencing<br />
Detection systems:<br />
Fluorescence, chemoluminescence, radioactivity, colour<br />
Pore size Binding Dimensions L x W Art. No. Pack Qty. €<br />
(μm)<br />
(cm)<br />
0.2 hydrophobic 20 x 13 2831.1 2 sheets 32,05<br />
0.2 hydrophobic 330 x 26 2803.1 1 roll 291,35