Electrophoresis
Electrophoresis
Electrophoresis
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Elektrophorese<br />
<strong>Electrophoresis</strong> · Blotting Reagents<br />
Roti ® -Green<br />
ready-to-use<br />
Sensitive fluorescent staining for protein gels and Blot membranes.<br />
Store at +4 °C<br />
• Suitable for staining of all gels and blots<br />
• Recommended for 2D-gels and prior to MS-analysis<br />
• High sensitivity (3 ng/mm 2 )<br />
• Completely reversible, non-fixing<br />
• Superior price/performance ratio<br />
Roti ® -Green staining is based on the formation of metal chelate-detergent<br />
complexes with the proteins and is completely reversible. The high sensitivity<br />
of the staining and the linear correlation of signal to protein amount over<br />
a broad range makes Roti ® -Green a universally suitable staining reagent.<br />
Applications:<br />
Roti ® -Green can be used for the analysis of peptide size and protein<br />
content, as well as for gel staining prior to protein elution or MS-analyses<br />
in 2D-gel electrophoresis/proteomics. Roti ® -Green stains even membrane<br />
proteins independently of their amino acid composition. Additionally,<br />
Roti ® -Green can also be used for reversible staining of proteins transferred<br />
onto a Blotting-membrane. Sensitivity is approx. 3 ng/mm 2 and is, therefore,<br />
according to the internationally required standard. Signals can easily be<br />
documented using a long wave ethidium bromide transilluminator.<br />
Figure:<br />
B: Example of a Roti ® -Green stained protein blot,<br />
Lanes 1-9: Protein marker diluted in a twofold fashion. 1: 3.6 µg, 2: 1.8 µg,<br />
3: 0.9 µg, 4: 0.45 µg, 5: 0.225 µg, 6: 112 ng, 7: 56 ng, 8: 28 ng.<br />
Excitation maximum: 365 nm<br />
Emission maximum: 520 nm<br />
Documentation: standard SYBR Green photo filter system<br />
Sufficient for approx. 30 mini gels.<br />
For destaining of gels we recommend the use of UV-transparent gel staining<br />
trays (Art. No. CP59 and CP60).<br />
1000.1 1 l plastic 170,95 €<br />
Original pack 6 x 1 l 162,40/1 l<br />
Please note our Technical Information Brochure on the Internet<br />
(Life Science Main Page):<br />
Transfer Buffers<br />
Tips and protocols<br />
902<br />
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Roti ® -Stock 20x SSC<br />
ready-to-use, steam sterilised<br />
For molecular biology.<br />
SSC 20x ready-to-use solution<br />
20fold stock solution sodium/sodiumcitrate. Undiluted or after dilution<br />
with sterile water to 10x, 6x, 1x or 0.1x SSC one receives a versatile<br />
buffer for blotting, hybridising or washing for Southern or Dot/Slot-Blots<br />
of DNA as well as for in situ hybridisation.<br />
Undiluted buffer is also suitable for neutral transfer of RNA onto unloaded<br />
membranes (e.g. Roti ® -Nylon 0.2, Art. No. AE50.1).<br />
With original seal.<br />
Composition:<br />
3 M NaCl, 300 mM sodium citrate in deionised water, pH-value 7.0, steam sterilised<br />
1054.1 1 l glass 42,90 €<br />
Original pack 6 x 1 l 40,76/1 l<br />
Roti ® -Stock 20 % SDS<br />
Global Step - Email : Office@global-step.net<br />
Telefon : (+4) 0742 095 539<br />
Tel/Fax : (+4) 031 104 85 87<br />
Mobil : (+4) 0720 095 539, 0723 442 593<br />
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ready-to-use, 0.2 µm filtered<br />
For molecular biology and electrophoresis.<br />
SDS 20 % ready-to-use solution<br />
g Warning H315-H319<br />
20 % stock solution sodium dodecyl sulphate. Store the solution<br />
away from light in a dry place. SDS-stock solution and other solutions<br />
containing SDS should not be cooled below 24 °C otherwise the<br />
SDS may precipitate. The crystals can be easily resolubilised by<br />
heating them between 30 to 50 °C.<br />
With original seal.<br />
Composition:<br />
20 % SDS in deionised water, 0.2 µm filtered<br />
1057.1 1 l glass 107,40 €<br />
Original pack 6 x 1 l 102,03/1 l<br />
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